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The epitope specificity and tissue reactivity of four murine monoclonal anti-CD22 antibodies.
Cell Immunol. 1989 Jan; 118(1):85-99.CI

Abstract

The CD22 antigen is expressed on the surface of normal human B cells and some neoplastic B cell lines and tumors. Previous cross-blocking studies using a panel of monoclonal anti-CD22 antibodies have defined four epitope groups, termed A-D. In the present studies, we have further dissected the epitopes recognized by four monoclonal anti-CD22 antibodies using immunoprecipitation and cross-blocking techniques, immunofluorescence analyses with a variety of cell lines, and immunoperoxidase analyses of 36 normal human tissues. Two of the antibodies, HD6 and RFB4, have been described previously, and two, UV22-1 and UV22-2, are described in this report. Our studies indicate that the four monoclonal antibodies show unexpected complexities in their reactivity with CD22+ and CD22- cells and their reactivity with solubilized CD22 molecules. The four antibodies, which recognize epitopes defined previously as CD22-A and CD22-B, further subdivide these epitope clusters into four determinants, A1, A2, B1, and B2. Furthermore, only two of the antibodies, RFB4 and UV22-2, are B cell-specific. In summary, our data indicate that RFB4 and UV22-2 would be the antibodies of choice for constructing immunotoxins to treat B cell tumors.

Authors+Show Affiliations

Department of Microbiology, University of Texas Southwestern Medical Center, Dallas 75235.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2463099

Citation

Li, J L., et al. "The Epitope Specificity and Tissue Reactivity of Four Murine Monoclonal anti-CD22 Antibodies." Cellular Immunology, vol. 118, no. 1, 1989, pp. 85-99.
Li JL, Shen GL, Ghetie MA, et al. The epitope specificity and tissue reactivity of four murine monoclonal anti-CD22 antibodies. Cell Immunol. 1989;118(1):85-99.
Li, J. L., Shen, G. L., Ghetie, M. A., May, R. D., Till, M., Ghetie, V., Uhr, J. W., Janossy, G., Thorpe, P. E., & Amlot, P. (1989). The epitope specificity and tissue reactivity of four murine monoclonal anti-CD22 antibodies. Cellular Immunology, 118(1), 85-99.
Li JL, et al. The Epitope Specificity and Tissue Reactivity of Four Murine Monoclonal anti-CD22 Antibodies. Cell Immunol. 1989;118(1):85-99. PubMed PMID: 2463099.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The epitope specificity and tissue reactivity of four murine monoclonal anti-CD22 antibodies. A1 - Li,J L, AU - Shen,G L, AU - Ghetie,M A, AU - May,R D, AU - Till,M, AU - Ghetie,V, AU - Uhr,J W, AU - Janossy,G, AU - Thorpe,P E, AU - Amlot,P, PY - 1989/1/1/pubmed PY - 1989/1/1/medline PY - 1989/1/1/entrez SP - 85 EP - 99 JF - Cellular immunology JO - Cell Immunol VL - 118 IS - 1 N2 - The CD22 antigen is expressed on the surface of normal human B cells and some neoplastic B cell lines and tumors. Previous cross-blocking studies using a panel of monoclonal anti-CD22 antibodies have defined four epitope groups, termed A-D. In the present studies, we have further dissected the epitopes recognized by four monoclonal anti-CD22 antibodies using immunoprecipitation and cross-blocking techniques, immunofluorescence analyses with a variety of cell lines, and immunoperoxidase analyses of 36 normal human tissues. Two of the antibodies, HD6 and RFB4, have been described previously, and two, UV22-1 and UV22-2, are described in this report. Our studies indicate that the four monoclonal antibodies show unexpected complexities in their reactivity with CD22+ and CD22- cells and their reactivity with solubilized CD22 molecules. The four antibodies, which recognize epitopes defined previously as CD22-A and CD22-B, further subdivide these epitope clusters into four determinants, A1, A2, B1, and B2. Furthermore, only two of the antibodies, RFB4 and UV22-2, are B cell-specific. In summary, our data indicate that RFB4 and UV22-2 would be the antibodies of choice for constructing immunotoxins to treat B cell tumors. SN - 0008-8749 UR - https://www.unboundmedicine.com/medline/citation/2463099/The_epitope_specificity_and_tissue_reactivity_of_four_murine_monoclonal_anti_CD22_antibodies_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0008-8749(89)90359-6 DB - PRIME DP - Unbound Medicine ER -