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Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China.
J Basic Microbiol 2014; 54(11):1273-8JB

Abstract

Orf is an exanthemous viral disease seriously threatening the goat and sheep industry and widely epidemic in the goat and sheep populations in Xinjiang, China. In order to investigate the genetic variability of the orf virus (ORFV), three virus isolates (SHZ1, SHZ2, and SHZ3) were isolated by PCR and Vero cell culture using the clinical samples from the lips of the lambs suspected of ORFV infection. The isolates were further verified by electron microscopy and animal infection experiments. The protective antigen genes B2L, F1L, and virulence genes VIR, GIF, and VEGF in the isolates were cloned, sequenced and analyzed for genetic evolution. The results showed that B2L and F1L were relatively conservative with homology 86.7-97.9%, while VIR, GIF, particularly VEGF were considerably variable with homology 71.5-97.9% at amino acid sequence level, respectively. Phylogenetic tree analysis based on B2L and VIR showed that the isolates SHZ1 and SHZ2 were closely related with the Taiwan isolates. This is the first report to confirm that there have been genetic variations in the Xinjiang ORFV isolates. The findings provide molecular evidence about the genetic variability of the major antigenic and virulence genes in the virus isolates epidemic in Xinjiang.

Authors+Show Affiliations

College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24633847

Citation

Yang, Haibo, et al. "Detection of Genetic Variations in Orf Virus Isolates Epidemic in Xinjiang China." Journal of Basic Microbiology, vol. 54, no. 11, 2014, pp. 1273-8.
Yang H, Meng Q, Qiao J, et al. Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China. J Basic Microbiol. 2014;54(11):1273-8.
Yang, H., Meng, Q., Qiao, J., Peng, Y., Xie, K., Liu, Y., ... Chen, C. (2014). Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China. Journal of Basic Microbiology, 54(11), pp. 1273-8. doi:10.1002/jobm.201300911.
Yang H, et al. Detection of Genetic Variations in Orf Virus Isolates Epidemic in Xinjiang China. J Basic Microbiol. 2014;54(11):1273-8. PubMed PMID: 24633847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of genetic variations in Orf virus isolates epidemic in Xinjiang China. AU - Yang,Haibo, AU - Meng,Qingling, AU - Qiao,Jun, AU - Peng,Yelong, AU - Xie,Kun, AU - Liu,Yucheng, AU - Zhao,Hailong, AU - Cai,Xuepeng, AU - Chen,Chuangfu, Y1 - 2014/03/14/ PY - 2013/11/13/received PY - 2014/02/12/accepted PY - 2014/3/18/entrez PY - 2014/3/19/pubmed PY - 2015/7/15/medline KW - Contagious ecthyma KW - Domestic ruminants KW - Genetic variability KW - Orf virus KW - Parapoxvirus KW - Sheep and goats KW - Zoonosis SP - 1273 EP - 8 JF - Journal of basic microbiology JO - J. Basic Microbiol. VL - 54 IS - 11 N2 - Orf is an exanthemous viral disease seriously threatening the goat and sheep industry and widely epidemic in the goat and sheep populations in Xinjiang, China. In order to investigate the genetic variability of the orf virus (ORFV), three virus isolates (SHZ1, SHZ2, and SHZ3) were isolated by PCR and Vero cell culture using the clinical samples from the lips of the lambs suspected of ORFV infection. The isolates were further verified by electron microscopy and animal infection experiments. The protective antigen genes B2L, F1L, and virulence genes VIR, GIF, and VEGF in the isolates were cloned, sequenced and analyzed for genetic evolution. The results showed that B2L and F1L were relatively conservative with homology 86.7-97.9%, while VIR, GIF, particularly VEGF were considerably variable with homology 71.5-97.9% at amino acid sequence level, respectively. Phylogenetic tree analysis based on B2L and VIR showed that the isolates SHZ1 and SHZ2 were closely related with the Taiwan isolates. This is the first report to confirm that there have been genetic variations in the Xinjiang ORFV isolates. The findings provide molecular evidence about the genetic variability of the major antigenic and virulence genes in the virus isolates epidemic in Xinjiang. SN - 1521-4028 UR - https://www.unboundmedicine.com/medline/citation/24633847/Detection_of_genetic_variations_in_Orf_virus_isolates_epidemic_in_Xinjiang_China_ L2 - https://doi.org/10.1002/jobm.201300911 DB - PRIME DP - Unbound Medicine ER -