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International Society for the Advancement of Cytometry cell sorter biosafety standards.
Cytometry A. 2014 May; 85(5):434-53.C

Abstract

Flow cytometric cell sorting of biological specimens has become prevalent in basic and clinical research laboratories. These specimens may contain known or unknown infectious agents, necessitating precautions to protect instrument operators and the environment from biohazards arising from the use of sorters. To this end the International Society of Analytical Cytology (ISAC) was proactive in establishing biosafety guidelines in 1997 (Schmid et al., Cytometry 1997;28:99-117) and subsequently published revised biosafety standards for cell sorting of unfixed samples in 2007 (Schmid et al., Cytometry Part A J Int Soc Anal Cytol 2007;71A:414-437). Since their publication, these documents have become recognized worldwide as the standard of practice and safety precautions for laboratories performing cell sorting experiments. However, the field of cytometry has progressed since 2007, and the document requires an update. The new Standards provides guidance: (1) for laboratory design for cell sorter laboratories; (2) for the creation of laboratory or instrument specific Standard Operating Procedures (SOP); and (3) on procedures for the safe operation of cell sorters, including personal protective equipment (PPE) and validation of aerosol containment.

Links

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Authors+Show Affiliations

Holmes KL
Flow Cytometry Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
Fontes B
No affiliation info available
Hogarth P
No affiliation info available
Konz R
No affiliation info available
Monard S
No affiliation info available
Pletcher CH
No affiliation info available
Wadley RB
No affiliation info available
Schmid I
No affiliation info available
Perfetto SP
No affiliation info available

MeSH

Cell SeparationEquipment ContaminationFlow CytometryHazardous SubstancesHumansLaboratoriesOccupational HealthSafety ManagementSocieties, Scientific

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

24634405

Citation

Holmes, Kevin L., et al. "International Society for the Advancement of Cytometry Cell Sorter Biosafety Standards." Cytometry. Part a : the Journal of the International Society for Analytical Cytology, vol. 85, no. 5, 2014, pp. 434-53.
Holmes KL, Fontes B, Hogarth P, et al. International Society for the Advancement of Cytometry cell sorter biosafety standards. Cytometry A. 2014;85(5):434-53.
Holmes, K. L., Fontes, B., Hogarth, P., Konz, R., Monard, S., Pletcher, C. H., Wadley, R. B., Schmid, I., & Perfetto, S. P. (2014). International Society for the Advancement of Cytometry cell sorter biosafety standards. Cytometry. Part a : the Journal of the International Society for Analytical Cytology, 85(5), 434-53. https://doi.org/10.1002/cyto.a.22454
Holmes KL, et al. International Society for the Advancement of Cytometry Cell Sorter Biosafety Standards. Cytometry A. 2014;85(5):434-53. PubMed PMID: 24634405.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - International Society for the Advancement of Cytometry cell sorter biosafety standards. AU - Holmes,Kevin L, AU - Fontes,Benjamin, AU - Hogarth,Philip, AU - Konz,Richard, AU - Monard,Simon, AU - Pletcher,Charles H,Jr AU - Wadley,Robert B, AU - Schmid,Ingrid, AU - Perfetto,Stephen P, Y1 - 2014/03/13/ PY - 2013/09/24/received PY - 2014/01/17/revised PY - 2014/02/16/accepted PY - 2014/3/18/entrez PY - 2014/3/19/pubmed PY - 2014/12/15/medline KW - aerosol containment KW - biohazards KW - biosafety KW - cell sorting KW - flow cytometry KW - occupational health SP - 434 EP - 53 JF - Cytometry. Part A : the journal of the International Society for Analytical Cytology JO - Cytometry A VL - 85 IS - 5 N2 - Flow cytometric cell sorting of biological specimens has become prevalent in basic and clinical research laboratories. These specimens may contain known or unknown infectious agents, necessitating precautions to protect instrument operators and the environment from biohazards arising from the use of sorters. To this end the International Society of Analytical Cytology (ISAC) was proactive in establishing biosafety guidelines in 1997 (Schmid et al., Cytometry 1997;28:99-117) and subsequently published revised biosafety standards for cell sorting of unfixed samples in 2007 (Schmid et al., Cytometry Part A J Int Soc Anal Cytol 2007;71A:414-437). Since their publication, these documents have become recognized worldwide as the standard of practice and safety precautions for laboratories performing cell sorting experiments. However, the field of cytometry has progressed since 2007, and the document requires an update. The new Standards provides guidance: (1) for laboratory design for cell sorter laboratories; (2) for the creation of laboratory or instrument specific Standard Operating Procedures (SOP); and (3) on procedures for the safe operation of cell sorters, including personal protective equipment (PPE) and validation of aerosol containment. SN - 1552-4930 UR - https://www.unboundmedicine.com/medline/citation/24634405/International_Society_for_the_Advancement_of_Cytometry_cell_sorter_biosafety_standards_ DB - PRIME DP - Unbound Medicine ER -