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An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract.
Chin J Nat Med. 2014 Feb; 12(2):154-60.CJ

Abstract

AIMS

To develop an HPLC-MS/MS method for the quantification of platycodin D (PD) in rat plasma, and to acquire the main pharmacokinetic parameters of PD after oral administration of pure PD or of Platycodi Radix extract (PRE) containing PD.

METHOD

Plasma samples were pretreated with solid-phase extraction using an Oasis® HLB SPE cartridge. Madecassoside was used as the internal standard (IS). Chromatographic separation was achieved on an ODS column (100 mm × 2.1 mm i.d., 3.5 μm) with a mobile phase consisting of acetonitrile/water (30 : 70, V/V) containing 0.1 mmol·L(-1) ammonium acetate at a flow rate of 0.25 mL·min(-1). The detection was performed on a triple quadruple tandem mass spectrometer using an electrospray ionization (ESI) source with a chromatographic run time of 3.0 min. The detection was operated by multiple reaction monitoring (MRM) of the transitions of m/z 1 223.6→469.2 for PD and of m/z 973.6→469.2 for madecassoside (IS), respectively.

RESULTS

The calibration curve was linear from 5 to 2 000 ng·mL(-1) (r(2) >0.99) with a lower limit of quantification (LLOQ) of 5 ng·mL(-1). The intra- and inter-day precision (relative standard deviation, RSD) values were below 15% and the accuracy (relative error, RE) was from -15% to +15% at three quality control (QC) levels. Plasma concentrations of PD were determined for 24 h after i.v. administration of PD, and oral administration of PD and PRE, respectively. The absolute oral bioavailability of PD in rats was found to be (0.48 ± 0.19)% when administered PD, and to be (1.81 ± 0.89)% when administered PRE.

CONCLUSION

The developed HPLC-MS/MS method was successfully applied to assess the pharmacokinetic parameters and oral bioavailability of PD in rats after administration of PD and Platycodi Radix extract.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhan Q
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
Zhang F
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
Gao SH
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
Cai F
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
Jiang B
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China.
Sun LN
Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China; Department of Pharmacognosy, School of Pharmacy, Second Military Medical University, Shanghai 200433, China.
Chen WS
Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China; Modern Research Center for Traditional Chinese Medicine, Second Military Medical University, Shanghai 200433, China. Electronic address: chenwanshengsmmu@aliyun.com.

MeSH

Administration, OralAnimalsBiological AvailabilityChromatography, High Pressure LiquidDrugs, Chinese HerbalMalePlant RootsPlatycodonRatsRats, Sprague-DawleySaponinsTandem Mass SpectrometryTriterpenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24636069

Citation

Zhan, Qin, et al. "An HPLC-MS/MS Method for the Quantitative Determination of Platycodin D in Rat Plasma and Its Application to the Pharmacokinetics of Platycodi Radix Extract." Chinese Journal of Natural Medicines, vol. 12, no. 2, 2014, pp. 154-60.
Zhan Q, Zhang F, Gao SH, et al. An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract. Chin J Nat Med. 2014;12(2):154-60.
Zhan, Q., Zhang, F., Gao, S. H., Cai, F., Jiang, B., Sun, L. N., & Chen, W. S. (2014). An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract. Chinese Journal of Natural Medicines, 12(2), 154-60. https://doi.org/10.1016/S1875-5364(14)60026-1
Zhan Q, et al. An HPLC-MS/MS Method for the Quantitative Determination of Platycodin D in Rat Plasma and Its Application to the Pharmacokinetics of Platycodi Radix Extract. Chin J Nat Med. 2014;12(2):154-60. PubMed PMID: 24636069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An HPLC-MS/MS method for the quantitative determination of platycodin D in rat plasma and its application to the pharmacokinetics of Platycodi Radix extract. AU - Zhan,Qin, AU - Zhang,Feng, AU - Gao,Shou-Hong, AU - Cai,Fei, AU - Jiang,Bo, AU - Sun,Lian-Na, AU - Chen,Wan-Sheng, PY - 2012/09/20/received PY - 2014/3/19/entrez PY - 2014/3/19/pubmed PY - 2014/12/15/medline KW - HPLC-MS/MS KW - Pharmacokinetics KW - Platycodi Radix KW - Platycodin D KW - Platycodon grandiflorus KW - Rat plasma SP - 154 EP - 60 JF - Chinese journal of natural medicines JO - Chin J Nat Med VL - 12 IS - 2 N2 - AIMS: To develop an HPLC-MS/MS method for the quantification of platycodin D (PD) in rat plasma, and to acquire the main pharmacokinetic parameters of PD after oral administration of pure PD or of Platycodi Radix extract (PRE) containing PD. METHOD: Plasma samples were pretreated with solid-phase extraction using an Oasis® HLB SPE cartridge. Madecassoside was used as the internal standard (IS). Chromatographic separation was achieved on an ODS column (100 mm × 2.1 mm i.d., 3.5 μm) with a mobile phase consisting of acetonitrile/water (30 : 70, V/V) containing 0.1 mmol·L(-1) ammonium acetate at a flow rate of 0.25 mL·min(-1). The detection was performed on a triple quadruple tandem mass spectrometer using an electrospray ionization (ESI) source with a chromatographic run time of 3.0 min. The detection was operated by multiple reaction monitoring (MRM) of the transitions of m/z 1 223.6→469.2 for PD and of m/z 973.6→469.2 for madecassoside (IS), respectively. RESULTS: The calibration curve was linear from 5 to 2 000 ng·mL(-1) (r(2) >0.99) with a lower limit of quantification (LLOQ) of 5 ng·mL(-1). The intra- and inter-day precision (relative standard deviation, RSD) values were below 15% and the accuracy (relative error, RE) was from -15% to +15% at three quality control (QC) levels. Plasma concentrations of PD were determined for 24 h after i.v. administration of PD, and oral administration of PD and PRE, respectively. The absolute oral bioavailability of PD in rats was found to be (0.48 ± 0.19)% when administered PD, and to be (1.81 ± 0.89)% when administered PRE. CONCLUSION: The developed HPLC-MS/MS method was successfully applied to assess the pharmacokinetic parameters and oral bioavailability of PD in rats after administration of PD and Platycodi Radix extract. SN - 1875-5364 UR - https://www.unboundmedicine.com/medline/citation/24636069/An_HPLC_MS/MS_method_for_the_quantitative_determination_of_platycodin_D_in_rat_plasma_and_its_application_to_the_pharmacokinetics_of_Platycodi_Radix_extract_ DB - PRIME DP - Unbound Medicine ER -
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