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Simultaneous determination of Guanfu base G and its active metabolites by UPLC-MS/MS in rat plasma and its application to a pharmacokinetic study.

Abstract

To establish a rapid and sensitive ultra performance liquid chromatography mass spectrometry (UPLC-MS/MS) method for the determination of concentration of guanfu base G (GFG) and its active metabolites in rat plasma. The GFG and its active metabolites and the internal standard (phenacetin) were separated on an Acquity UPLC(®) BEH C18 chromatography column (2.1mm×50mm I.D., 1.7μm) using gradient elution with a mobile phase of methanol and ultrapure water at a flow rate of 0.4mL/min. The detection was performed on a Xevo triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 472.26→m/z 310.03 for GFG and m/z 180.00→m/z 109.99 for phenacetin (IS) using a positive electrospray ionization interface. The lower limit of quantification (LLOQ) was 1ng/mL, the limit of detection (LOD) was 0.3ng/mL, and the linear calibration curve was obtained over the concentration range of 1-200ng/mL. The intra-day and inter-day assay variations were measured to be below 10.97%, and the accuracy values (relative error) ranged from 95.4% to 103.6%. After validation, this method was successfully applied to a pharmacokinetic study where rats were intravenous administration of 5mg/kg GFG. A simple, rapid, sensitive, and accurate method for the determination of the concentration of GFG and its metabolites in rat plasma was developed and validated.

Authors+Show Affiliations

Department of Pharmacy, Zhengzhou University, Zhengzhou 450001, China; Henan Provincial People's Hospital, Zhengzhou 450003, China.Department of Pharmacy, Zhengzhou University, Zhengzhou 450001, China.The Henan Provincial Food and Drug Inspection Office, Zhengzhou 450003, China.Department of Pharmacy, Zhengzhou University, Zhengzhou 450001, China.Department of Pharmacy, Zhengzhou University, Zhengzhou 450001, China. Electronic address: lixt@zzu.edu.cn.Department of Pharmacy, Zhengzhou University, Zhengzhou 450001, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24636893

Citation

Zhang, Lingchun, et al. "Simultaneous Determination of Guanfu Base G and Its Active Metabolites By UPLC-MS/MS in Rat Plasma and Its Application to a Pharmacokinetic Study." Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, vol. 957, 2014, pp. 1-6.
Zhang L, Wang A, Wang X, et al. Simultaneous determination of Guanfu base G and its active metabolites by UPLC-MS/MS in rat plasma and its application to a pharmacokinetic study. J Chromatogr B Analyt Technol Biomed Life Sci. 2014;957:1-6.
Zhang, L., Wang, A., Wang, X., Zhang, Y., Li, X., & Liu, P. (2014). Simultaneous determination of Guanfu base G and its active metabolites by UPLC-MS/MS in rat plasma and its application to a pharmacokinetic study. Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 957, 1-6. https://doi.org/10.1016/j.jchromb.2014.02.024
Zhang L, et al. Simultaneous Determination of Guanfu Base G and Its Active Metabolites By UPLC-MS/MS in Rat Plasma and Its Application to a Pharmacokinetic Study. J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Apr 15;957:1-6. PubMed PMID: 24636893.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of Guanfu base G and its active metabolites by UPLC-MS/MS in rat plasma and its application to a pharmacokinetic study. AU - Zhang,Lingchun, AU - Wang,Aiqin, AU - Wang,Xueqin, AU - Zhang,Ying, AU - Li,Xiaotian, AU - Liu,Ping, Y1 - 2014/02/22/ PY - 2013/12/31/received PY - 2014/02/11/revised PY - 2014/02/15/accepted PY - 2014/3/19/entrez PY - 2014/3/19/pubmed PY - 2014/11/11/medline KW - Active metabolites KW - Guanfu base G KW - Pharmacokinetic KW - Rat plasma KW - UPLC–MS/MS SP - 1 EP - 6 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. VL - 957 N2 - To establish a rapid and sensitive ultra performance liquid chromatography mass spectrometry (UPLC-MS/MS) method for the determination of concentration of guanfu base G (GFG) and its active metabolites in rat plasma. The GFG and its active metabolites and the internal standard (phenacetin) were separated on an Acquity UPLC(®) BEH C18 chromatography column (2.1mm×50mm I.D., 1.7μm) using gradient elution with a mobile phase of methanol and ultrapure water at a flow rate of 0.4mL/min. The detection was performed on a Xevo triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) mode to monitor the precursor-to-product ion transitions of m/z 472.26→m/z 310.03 for GFG and m/z 180.00→m/z 109.99 for phenacetin (IS) using a positive electrospray ionization interface. The lower limit of quantification (LLOQ) was 1ng/mL, the limit of detection (LOD) was 0.3ng/mL, and the linear calibration curve was obtained over the concentration range of 1-200ng/mL. The intra-day and inter-day assay variations were measured to be below 10.97%, and the accuracy values (relative error) ranged from 95.4% to 103.6%. After validation, this method was successfully applied to a pharmacokinetic study where rats were intravenous administration of 5mg/kg GFG. A simple, rapid, sensitive, and accurate method for the determination of the concentration of GFG and its metabolites in rat plasma was developed and validated. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/24636893/Simultaneous_determination_of_Guanfu_base_G_and_its_active_metabolites_by_UPLC_MS/MS_in_rat_plasma_and_its_application_to_a_pharmacokinetic_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(14)00106-8 DB - PRIME DP - Unbound Medicine ER -