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Simultaneous determination of puerarin, daidzin, daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of Ge-Gen Decoction by a liquid chromatography-electrospray ionization-tandem mass spectrometry.
J Pharm Biomed Anal. 2014 Jul; 95:76-84.JP

Abstract

A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5→297.2 for puerarin, m/z 417.1→255.2 for daidzin, m/z 255.2→152.4 for daidzein, m/z 498.1→179.3 for paeoniflorin, m/z 481.1→197.3 for albiflorin, m/z 436.2→257.3 for liquiritin, m/z 257.2→137.3 for liquiritigenin and m/z 415.0→384.2 for IS, respectively. All calibration curves exhibited good linearity (r>0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from -13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract.

Authors+Show Affiliations

Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China.Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.Jiangsu Province Academy of Traditional Chinese Medicine, Nanjing 210028, China.State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China.Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 211198, China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, China. Electronic address: boyangyu59@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24637051

Citation

Yan, Yan, et al. "Simultaneous Determination of Puerarin, Daidzin, Daidzein, Paeoniflorin, Albiflorin, Liquiritin and Liquiritigenin in Rat Plasma and Its Application to a Pharmacokinetic Study of Ge-Gen Decoction By a Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry." Journal of Pharmaceutical and Biomedical Analysis, vol. 95, 2014, pp. 76-84.
Yan Y, Chai CZ, Wang DW, et al. Simultaneous determination of puerarin, daidzin, daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of Ge-Gen Decoction by a liquid chromatography-electrospray ionization-tandem mass spectrometry. J Pharm Biomed Anal. 2014;95:76-84.
Yan, Y., Chai, C. Z., Wang, D. W., Wu, J., Xiao, H. H., Huo, L. X., Zhu, D. N., & Yu, B. Y. (2014). Simultaneous determination of puerarin, daidzin, daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of Ge-Gen Decoction by a liquid chromatography-electrospray ionization-tandem mass spectrometry. Journal of Pharmaceutical and Biomedical Analysis, 95, 76-84. https://doi.org/10.1016/j.jpba.2014.02.013
Yan Y, et al. Simultaneous Determination of Puerarin, Daidzin, Daidzein, Paeoniflorin, Albiflorin, Liquiritin and Liquiritigenin in Rat Plasma and Its Application to a Pharmacokinetic Study of Ge-Gen Decoction By a Liquid Chromatography-electrospray Ionization-tandem Mass Spectrometry. J Pharm Biomed Anal. 2014;95:76-84. PubMed PMID: 24637051.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of puerarin, daidzin, daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma and its application to a pharmacokinetic study of Ge-Gen Decoction by a liquid chromatography-electrospray ionization-tandem mass spectrometry. AU - Yan,Yan, AU - Chai,Cheng-Zhi, AU - Wang,Da-Wei, AU - Wu,Jie, AU - Xiao,Hong-He, AU - Huo,Li-Xia, AU - Zhu,Dan-Ni, AU - Yu,Bo-Yang, Y1 - 2014/03/02/ PY - 2013/12/03/received PY - 2014/02/17/revised PY - 2014/02/20/accepted PY - 2014/3/19/entrez PY - 2014/3/19/pubmed PY - 2015/1/31/medline KW - Ge-Gen Decoction KW - Isoflavones KW - LC–ESI-MS/MS KW - Monoterpene glucosides KW - Pharmacokinetics SP - 76 EP - 84 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 95 N2 - A liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method was developed and validated for simultaneous determination of seven constituents including puerarin, daidzin, daidzein, paeoniflorin, albiflorin, liquiritin and liquiritigenin in rat plasma using schisandrin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with acetonitrile. The chromatographic separation was carried out on a C18 column with a gradient mobile phase consisting of acetonitrile and water (containing 0.1% formic acid and 5mM ammonium acetate). All analytes and IS were quantitated through electrospray ionization in positive ion multiple reaction monitoring (MRM) mode. The mass transitions were as follows: m/z 417.5→297.2 for puerarin, m/z 417.1→255.2 for daidzin, m/z 255.2→152.4 for daidzein, m/z 498.1→179.3 for paeoniflorin, m/z 481.1→197.3 for albiflorin, m/z 436.2→257.3 for liquiritin, m/z 257.2→137.3 for liquiritigenin and m/z 415.0→384.2 for IS, respectively. All calibration curves exhibited good linearity (r>0.9979) over a wide concentration range for all components. The intra-day and inter-day precisions (RSD) at three different levels were both less than 14.3% and the accuracies (RE) ranged from -13.2% to 14.8%. The extraction recoveries of the seven compounds ranged from 72.9% to 117.4%. The validated method was successfully applied to pharmacokinetic study of the seven components in female rat plasma after oral administration of Ge-Gen Decoction aqueous extract. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/24637051/Simultaneous_determination_of_puerarin_daidzin_daidzein_paeoniflorin_albiflorin_liquiritin_and_liquiritigenin_in_rat_plasma_and_its_application_to_a_pharmacokinetic_study_of_Ge_Gen_Decoction_by_a_liquid_chromatography_electrospray_ionization_tandem_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -