Tags

Type your tag names separated by a space and hit enter

Field-evolved resistance by western corn rootworm to multiple Bacillus thuringiensis toxins in transgenic maize.
Proc Natl Acad Sci U S A. 2014 Apr 08; 111(14):5141-6.PN

Abstract

The widespread planting of crops genetically engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) places intense selective pressure on pest populations to evolve resistance. Western corn rootworm is a key pest of maize, and in continuous maize fields it is often managed through planting of Bt maize. During 2009 and 2010, fields were identified in Iowa in which western corn rootworm imposed severe injury to maize producing Bt toxin Cry3Bb1. Subsequent bioassays revealed Cry3Bb1 resistance in these populations. Here, we report that, during 2011, injury to Bt maize in the field expanded to include mCry3A maize in addition to Cry3Bb1 maize and that laboratory analysis of western corn rootworm from these fields found resistance to Cry3Bb1 and mCry3A and cross-resistance between these toxins. Resistance to Bt maize has persisted in Iowa, with both the number of Bt fields identified with severe root injury and the ability western corn rootworm populations to survive on Cry3Bb1 maize increasing between 2009 and 2011. Additionally, Bt maize targeting western corn rootworm does not produce a high dose of Bt toxin, and the magnitude of resistance associated with feeding injury was less than that seen in a high-dose Bt crop. These first cases of resistance by western corn rootworm highlight the vulnerability of Bt maize to further evolution of resistance from this pest and, more broadly, point to the potential of insects to develop resistance rapidly when Bt crops do not achieve a high dose of Bt toxin.

Links

PMC Free PDF

Authors+Show Affiliations

Gassmann AJ
Department of Entomology, Iowa State University, Ames, IA 50011.
Petzold-Maxwell JL
No affiliation info available
Clifton EH
No affiliation info available
Dunbar MW
No affiliation info available
Hoffmann AM
No affiliation info available
Ingber DA
No affiliation info available
Keweshan RS
No affiliation info available

MeSH

AnimalsBacillus thuringiensisBacterial ToxinsColeopteraPlants, Genetically ModifiedZea mays

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24639498

Citation

Gassmann, Aaron J., et al. "Field-evolved Resistance By Western Corn Rootworm to Multiple Bacillus Thuringiensis Toxins in Transgenic Maize." Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 14, 2014, pp. 5141-6.
Gassmann AJ, Petzold-Maxwell JL, Clifton EH, et al. Field-evolved resistance by western corn rootworm to multiple Bacillus thuringiensis toxins in transgenic maize. Proc Natl Acad Sci U S A. 2014;111(14):5141-6.
Gassmann, A. J., Petzold-Maxwell, J. L., Clifton, E. H., Dunbar, M. W., Hoffmann, A. M., Ingber, D. A., & Keweshan, R. S. (2014). Field-evolved resistance by western corn rootworm to multiple Bacillus thuringiensis toxins in transgenic maize. Proceedings of the National Academy of Sciences of the United States of America, 111(14), 5141-6. https://doi.org/10.1073/pnas.1317179111
Gassmann AJ, et al. Field-evolved Resistance By Western Corn Rootworm to Multiple Bacillus Thuringiensis Toxins in Transgenic Maize. Proc Natl Acad Sci U S A. 2014 Apr 8;111(14):5141-6. PubMed PMID: 24639498.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Field-evolved resistance by western corn rootworm to multiple Bacillus thuringiensis toxins in transgenic maize. AU - Gassmann,Aaron J, AU - Petzold-Maxwell,Jennifer L, AU - Clifton,Eric H, AU - Dunbar,Mike W, AU - Hoffmann,Amanda M, AU - Ingber,David A, AU - Keweshan,Ryan S, Y1 - 2014/03/17/ PY - 2014/3/19/entrez PY - 2014/3/19/pubmed PY - 2014/6/17/medline KW - evolution KW - resistance management KW - transgenic crops SP - 5141 EP - 6 JF - Proceedings of the National Academy of Sciences of the United States of America JO - Proc Natl Acad Sci U S A VL - 111 IS - 14 N2 - The widespread planting of crops genetically engineered to produce insecticidal toxins derived from the bacterium Bacillus thuringiensis (Bt) places intense selective pressure on pest populations to evolve resistance. Western corn rootworm is a key pest of maize, and in continuous maize fields it is often managed through planting of Bt maize. During 2009 and 2010, fields were identified in Iowa in which western corn rootworm imposed severe injury to maize producing Bt toxin Cry3Bb1. Subsequent bioassays revealed Cry3Bb1 resistance in these populations. Here, we report that, during 2011, injury to Bt maize in the field expanded to include mCry3A maize in addition to Cry3Bb1 maize and that laboratory analysis of western corn rootworm from these fields found resistance to Cry3Bb1 and mCry3A and cross-resistance between these toxins. Resistance to Bt maize has persisted in Iowa, with both the number of Bt fields identified with severe root injury and the ability western corn rootworm populations to survive on Cry3Bb1 maize increasing between 2009 and 2011. Additionally, Bt maize targeting western corn rootworm does not produce a high dose of Bt toxin, and the magnitude of resistance associated with feeding injury was less than that seen in a high-dose Bt crop. These first cases of resistance by western corn rootworm highlight the vulnerability of Bt maize to further evolution of resistance from this pest and, more broadly, point to the potential of insects to develop resistance rapidly when Bt crops do not achieve a high dose of Bt toxin. SN - 1091-6490 UR - https://www.unboundmedicine.com/medline/citation/24639498/Field_evolved_resistance_by_western_corn_rootworm_to_multiple_Bacillus_thuringiensis_toxins_in_transgenic_maize_ DB - PRIME DP - Unbound Medicine ER -