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[Peculiarity of reassortment of current wild type influenza viruses with master donor viruses for live influenza vaccine].
Vopr Virusol. 2013 Sep-Oct; 58(5):26-31.VV

Abstract

The live attenuated influenza vaccine (LAIV) currently licensed in Russia consists of the reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments from the circulating wild-type viruses and the six internal protein-encoding gene segments from cold-adapted master donor viruses (MDV) A/Leningrad/134/17/57 (H2N2) or B/USSR/60/69. Presently, only classical reassortment technique is approved for the generation of Russian LAIV strains. In this work, we describe the obstacles to the development of LAIV 6:2 vaccine strains depending on the phenotypic properties of the wild-type viruses used for reassortment. It was demonstrated that the highest percentage of 6:2 vaccine reassortants could be achieved when wild-type parental viruses were resistant to non-specific gamma-inhibitors. It was shown that it was impossible to generate 6:2 vaccine reassortants possessing six internal genes of the AILeningrad113417/57 (H2N2) master donor virus and avian HA and NA genes from H5N1-PR8 viruses using classical reassortment technique. It was suggested that strong constellation effects between the gene segments of the parental viruses could affect the virus gene reassortment. A strong interaction between the genome segments encoding neuraminidase of avian origin and PB2 gene of PR8 virus was observed. When the PB2 gene was inherited from cold-adapted master donor virus, the neuraminidase was also found to be of MDV origin.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

24640168

Citation

Kiseleva, I V., et al. "[Peculiarity of Reassortment of Current Wild Type Influenza Viruses With Master Donor Viruses for Live Influenza Vaccine]." Voprosy Virusologii, vol. 58, no. 5, 2013, pp. 26-31.
Kiseleva IV, Bazhenova EA, Larionova NV, et al. [Peculiarity of reassortment of current wild type influenza viruses with master donor viruses for live influenza vaccine]. Vopr Virusol. 2013;58(5):26-31.
Kiseleva, I. V., Bazhenova, E. A., Larionova, N. V., Fedorova, E. A., Dubrovina, I. A., Isakova-Sivak, I. N., & Rudenko, L. G. (2013). [Peculiarity of reassortment of current wild type influenza viruses with master donor viruses for live influenza vaccine]. Voprosy Virusologii, 58(5), 26-31.
Kiseleva IV, et al. [Peculiarity of Reassortment of Current Wild Type Influenza Viruses With Master Donor Viruses for Live Influenza Vaccine]. Vopr Virusol. 2013 Sep-Oct;58(5):26-31. PubMed PMID: 24640168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Peculiarity of reassortment of current wild type influenza viruses with master donor viruses for live influenza vaccine]. AU - Kiseleva,I V, AU - Bazhenova,E A, AU - Larionova,N V, AU - Fedorova,E A, AU - Dubrovina,I A, AU - Isakova-Sivak,I N, AU - Rudenko,L G, PY - 2014/3/20/entrez PY - 2014/3/20/pubmed PY - 2014/3/29/medline SP - 26 EP - 31 JF - Voprosy virusologii JO - Vopr Virusol VL - 58 IS - 5 N2 - The live attenuated influenza vaccine (LAIV) currently licensed in Russia consists of the reassortant viruses with hemagglutinin (HA) and neuraminidase (NA) gene segments from the circulating wild-type viruses and the six internal protein-encoding gene segments from cold-adapted master donor viruses (MDV) A/Leningrad/134/17/57 (H2N2) or B/USSR/60/69. Presently, only classical reassortment technique is approved for the generation of Russian LAIV strains. In this work, we describe the obstacles to the development of LAIV 6:2 vaccine strains depending on the phenotypic properties of the wild-type viruses used for reassortment. It was demonstrated that the highest percentage of 6:2 vaccine reassortants could be achieved when wild-type parental viruses were resistant to non-specific gamma-inhibitors. It was shown that it was impossible to generate 6:2 vaccine reassortants possessing six internal genes of the AILeningrad113417/57 (H2N2) master donor virus and avian HA and NA genes from H5N1-PR8 viruses using classical reassortment technique. It was suggested that strong constellation effects between the gene segments of the parental viruses could affect the virus gene reassortment. A strong interaction between the genome segments encoding neuraminidase of avian origin and PB2 gene of PR8 virus was observed. When the PB2 gene was inherited from cold-adapted master donor virus, the neuraminidase was also found to be of MDV origin. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/24640168/[Peculiarity_of_reassortment_of_current_wild_type_influenza_viruses_with_master_donor_viruses_for_live_influenza_vaccine]_ L2 - https://medlineplus.gov/birdflu.html DB - PRIME DP - Unbound Medicine ER -