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[Evaluation of the commercial ELISA test-systems of different formats to detect specific IgM and IgG in the measles patients sera].
Vopr Virusol. 2013 Sep-Oct; 58(5):43-8.VV

Abstract

Nine commercial kits of "captured" and "indirect" format ELISA assay for the detection of specific IgM and IgG in sera of patients with measles were compared to each other. 72 sera specimens from typical medium-severity cases from a measles outbreak (2010) were collected on the 5-6th day after the rash onset. IgM was detected with "capture" tests (Vecto-Measles IgM, Vector Best, Measles IgM capture EIA, Microimmune Ltd) close to 100% of cases, irrespectively to the age and the initial vaccination status of the patients. The IgM result was negative in 23.6% by average while investigating using "indirect" format tests (Enzygnost Anti-Measles Virusll/IgM, Siemens; Anti-Measles Viruses ELISA (IgM), Eurominimum, Virion-Serion IgM (GmbH). These cases were in adults, the majority of which had 1-2 vaccinations in the past. The analysis of the presented data shows high correlation connection between the tests used and high confidence level for OD IgM and IgG of the sera of the patients with the primary and secondary immune response.

Authors

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Pub Type(s)

English Abstract
Journal Article

Language

rus

PubMed ID

24640171

Citation

Mamaeva, T A., et al. "[Evaluation of the Commercial ELISA Test-systems of Different Formats to Detect Specific IgM and IgG in the Measles Patients Sera]." Voprosy Virusologii, vol. 58, no. 5, 2013, pp. 43-8.
Mamaeva TA, Naumova MA, Zheleznova NV, et al. [Evaluation of the commercial ELISA test-systems of different formats to detect specific IgM and IgG in the measles patients sera]. Vopr Virusol. 2013;58(5):43-8.
Mamaeva, T. A., Naumova, M. A., Zheleznova, N. V., Lipskaia, G. I. u., Mulders, M., & Featherstone, D. A. (2013). [Evaluation of the commercial ELISA test-systems of different formats to detect specific IgM and IgG in the measles patients sera]. Voprosy Virusologii, 58(5), 43-8.
Mamaeva TA, et al. [Evaluation of the Commercial ELISA Test-systems of Different Formats to Detect Specific IgM and IgG in the Measles Patients Sera]. Vopr Virusol. 2013 Sep-Oct;58(5):43-8. PubMed PMID: 24640171.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Evaluation of the commercial ELISA test-systems of different formats to detect specific IgM and IgG in the measles patients sera]. AU - Mamaeva,T A, AU - Naumova,M A, AU - Zheleznova,N V, AU - Lipskaia,G Iu, AU - Mulders,M, AU - Featherstone,D A, PY - 2014/3/20/entrez PY - 2014/3/20/pubmed PY - 2014/3/29/medline SP - 43 EP - 8 JF - Voprosy virusologii JO - Vopr Virusol VL - 58 IS - 5 N2 - Nine commercial kits of "captured" and "indirect" format ELISA assay for the detection of specific IgM and IgG in sera of patients with measles were compared to each other. 72 sera specimens from typical medium-severity cases from a measles outbreak (2010) were collected on the 5-6th day after the rash onset. IgM was detected with "capture" tests (Vecto-Measles IgM, Vector Best, Measles IgM capture EIA, Microimmune Ltd) close to 100% of cases, irrespectively to the age and the initial vaccination status of the patients. The IgM result was negative in 23.6% by average while investigating using "indirect" format tests (Enzygnost Anti-Measles Virusll/IgM, Siemens; Anti-Measles Viruses ELISA (IgM), Eurominimum, Virion-Serion IgM (GmbH). These cases were in adults, the majority of which had 1-2 vaccinations in the past. The analysis of the presented data shows high correlation connection between the tests used and high confidence level for OD IgM and IgG of the sera of the patients with the primary and secondary immune response. SN - 0507-4088 UR - https://www.unboundmedicine.com/medline/citation/24640171/[Evaluation_of_the_commercial_ELISA_test_systems_of_different_formats_to_detect_specific_IgM_and_IgG_in_the_measles_patients_sera]_ L2 - http://www.diseaseinfosearch.org/result/4535 DB - PRIME DP - Unbound Medicine ER -