Tags

Type your tag names separated by a space and hit enter

Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein. II. Suppression of disease and in vitro immune responses is mediated by antigen-specific CD8+ T lymphocytes.
J Immunol. 1989 Feb 01; 142(3):748-52.JI

Abstract

We have previously demonstrated that the oral administration of guinea pig myelin basic protein (MBP) protects Lewis rats against the induction of experimental autoimmune encephalomyelitis (EAE) when subsequently immunized with guinea pig MBP in CFA. In addition, animals made orally tolerant to MBP also have diminished proliferative and antibody responses to MBP, but not to other Ag. Nonetheless, the mechanism of oral tolerance to MBP in the EAE model remains undefined. In the present study, we report that T cells isolated from the spleen and mesenteric lymph nodes of MBP orally tolerized animals can adoptively transfer protection against EAE. Furthermore, these T cells are of the CD8+ subclass. In addition, CD8+ T cells from MBP orally tolerized animals also suppress in vitro proliferative responses and antibody responses to MBP in an Ag-specific fashion. These results demonstrate that active cellular mechanisms are initiated after oral administration of an autoantigen that can down-regulate an experimental autoimmune disease and provide the basis for the isolation and characterization of the cells mediating both in vivo and in vitro suppression.

Authors+Show Affiliations

Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2464023

Citation

Lider, O, et al. "Suppression of Experimental Autoimmune Encephalomyelitis By Oral Administration of Myelin Basic Protein. II. Suppression of Disease and in Vitro Immune Responses Is Mediated By Antigen-specific CD8+ T Lymphocytes." Journal of Immunology (Baltimore, Md. : 1950), vol. 142, no. 3, 1989, pp. 748-52.
Lider O, Santos LM, Lee CS, et al. Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein. II. Suppression of disease and in vitro immune responses is mediated by antigen-specific CD8+ T lymphocytes. J Immunol. 1989;142(3):748-52.
Lider, O., Santos, L. M., Lee, C. S., Higgins, P. J., & Weiner, H. L. (1989). Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein. II. Suppression of disease and in vitro immune responses is mediated by antigen-specific CD8+ T lymphocytes. Journal of Immunology (Baltimore, Md. : 1950), 142(3), 748-52.
Lider O, et al. Suppression of Experimental Autoimmune Encephalomyelitis By Oral Administration of Myelin Basic Protein. II. Suppression of Disease and in Vitro Immune Responses Is Mediated By Antigen-specific CD8+ T Lymphocytes. J Immunol. 1989 Feb 1;142(3):748-52. PubMed PMID: 2464023.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Suppression of experimental autoimmune encephalomyelitis by oral administration of myelin basic protein. II. Suppression of disease and in vitro immune responses is mediated by antigen-specific CD8+ T lymphocytes. AU - Lider,O, AU - Santos,L M, AU - Lee,C S, AU - Higgins,P J, AU - Weiner,H L, PY - 1989/2/1/pubmed PY - 1989/2/1/medline PY - 1989/2/1/entrez SP - 748 EP - 52 JF - Journal of immunology (Baltimore, Md. : 1950) JO - J. Immunol. VL - 142 IS - 3 N2 - We have previously demonstrated that the oral administration of guinea pig myelin basic protein (MBP) protects Lewis rats against the induction of experimental autoimmune encephalomyelitis (EAE) when subsequently immunized with guinea pig MBP in CFA. In addition, animals made orally tolerant to MBP also have diminished proliferative and antibody responses to MBP, but not to other Ag. Nonetheless, the mechanism of oral tolerance to MBP in the EAE model remains undefined. In the present study, we report that T cells isolated from the spleen and mesenteric lymph nodes of MBP orally tolerized animals can adoptively transfer protection against EAE. Furthermore, these T cells are of the CD8+ subclass. In addition, CD8+ T cells from MBP orally tolerized animals also suppress in vitro proliferative responses and antibody responses to MBP in an Ag-specific fashion. These results demonstrate that active cellular mechanisms are initiated after oral administration of an autoantigen that can down-regulate an experimental autoimmune disease and provide the basis for the isolation and characterization of the cells mediating both in vivo and in vitro suppression. SN - 0022-1767 UR - https://www.unboundmedicine.com/medline/citation/2464023/Suppression_of_experimental_autoimmune_encephalomyelitis_by_oral_administration_of_myelin_basic_protein__II__Suppression_of_disease_and_in_vitro_immune_responses_is_mediated_by_antigen_specific_CD8+_T_lymphocytes_ L2 - http://www.jimmunol.org/cgi/pmidlookup?view=long&pmid=2464023 DB - PRIME DP - Unbound Medicine ER -