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The effects of carvedilol on ischemia-reperfusion injury in the rat testis.
Int Braz J Urol. 2014 Jan-Feb; 40(1):109-17.IB

Abstract

OBJECTIVE

To analyze the oxidative damage and histopathological alterations caused by ischemia-reperfusion (I/R) injury and ameliorative effects of carvedilol (CVD) in the rat testis.

MATERIALS AND METHODS

Twenty-one male rats were randomized into 3 groups as follows: Group I (n = 7); control (sham) group, Group II (n = 7); I/R group, in which I/R injury was performed by torsing the left testis 720 ° clockwise for 2 hours and detorsing for 2 hours. Group III (n = 7); CVD treatment group; in addition to I/R process, one-dose of CVD was administered (2mg/kg, i.p) 30 min. before detorsion. Levels of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and levels of malondialdehyde (MDA) and protein carbonyl (PC) were determined in testicular tissues and serum of rats. Testicular tissues were also examined histopathologically and Johnsen scores were determined.

RESULTS

Activities of SOD and GSH-Px in serum and testicular tissues were increased by I/R, but administration of CVD decreased these levels (p < 0.001 and p = 0.001). Significantly increased MDA levels in serum and testicular tissues were decreased by CVD treatment (p < 0.001 and p = 0.001). Concerning PC levels in serum and testicular tissues, there was no statistically significant difference between the groups (p = 0.989 and p = 0.428). There was not a statistically significant difference in terms of mean Johnsen scores between the groups (p = 0.161).

CONCLUSIONS

Administration of CVD decreased oxidative damage biochemically in the rat testis caused by I/R injury, but histopathologically no change was observed betwe¬en all of the groups.

Authors+Show Affiliations

Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Pathology (FM), Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Biochemistry, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.Department of Urology, Faculty of Medicine, Gaziosmanpasa University, Tokat, Turkey.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24642157

Citation

Parlaktas, B S., et al. "The Effects of Carvedilol On Ischemia-reperfusion Injury in the Rat Testis." International Braz J Urol : Official Journal of the Brazilian Society of Urology, vol. 40, no. 1, 2014, pp. 109-17.
Parlaktas BS, Atilgan D, Gencten Y, et al. The effects of carvedilol on ischemia-reperfusion injury in the rat testis. Int Braz J Urol. 2014;40(1):109-17.
Parlaktas, B. S., Atilgan, D., Gencten, Y., Akbas, A., Markoc, F., Erdemir, F., Ozyurt, H., & Uluocak, N. (2014). The effects of carvedilol on ischemia-reperfusion injury in the rat testis. International Braz J Urol : Official Journal of the Brazilian Society of Urology, 40(1), 109-17. https://doi.org/10.1590/S1677-5538.IBJU.2014.01.16
Parlaktas BS, et al. The Effects of Carvedilol On Ischemia-reperfusion Injury in the Rat Testis. Int Braz J Urol. 2014 Jan-Feb;40(1):109-17. PubMed PMID: 24642157.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of carvedilol on ischemia-reperfusion injury in the rat testis. AU - Parlaktas,B S, AU - Atilgan,D, AU - Gencten,Y, AU - Akbas,A, AU - Markoc,F, AU - Erdemir,F, AU - Ozyurt,H, AU - Uluocak,N, PY - 2013/03/25/received PY - 2013/09/04/accepted PY - 2014/3/20/entrez PY - 2014/3/20/pubmed PY - 2014/10/1/medline SP - 109 EP - 17 JF - International braz j urol : official journal of the Brazilian Society of Urology JO - Int Braz J Urol VL - 40 IS - 1 N2 - OBJECTIVE: To analyze the oxidative damage and histopathological alterations caused by ischemia-reperfusion (I/R) injury and ameliorative effects of carvedilol (CVD) in the rat testis. MATERIALS AND METHODS: Twenty-one male rats were randomized into 3 groups as follows: Group I (n = 7); control (sham) group, Group II (n = 7); I/R group, in which I/R injury was performed by torsing the left testis 720 ° clockwise for 2 hours and detorsing for 2 hours. Group III (n = 7); CVD treatment group; in addition to I/R process, one-dose of CVD was administered (2mg/kg, i.p) 30 min. before detorsion. Levels of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and levels of malondialdehyde (MDA) and protein carbonyl (PC) were determined in testicular tissues and serum of rats. Testicular tissues were also examined histopathologically and Johnsen scores were determined. RESULTS: Activities of SOD and GSH-Px in serum and testicular tissues were increased by I/R, but administration of CVD decreased these levels (p < 0.001 and p = 0.001). Significantly increased MDA levels in serum and testicular tissues were decreased by CVD treatment (p < 0.001 and p = 0.001). Concerning PC levels in serum and testicular tissues, there was no statistically significant difference between the groups (p = 0.989 and p = 0.428). There was not a statistically significant difference in terms of mean Johnsen scores between the groups (p = 0.161). CONCLUSIONS: Administration of CVD decreased oxidative damage biochemically in the rat testis caused by I/R injury, but histopathologically no change was observed betwe¬en all of the groups. SN - 1677-6119 UR - https://www.unboundmedicine.com/medline/citation/24642157/The_effects_of_carvedilol_on_ischemia_reperfusion_injury_in_the_rat_testis_ L2 - http://brazjurol.com.br/january_february_2014//Parlaktas_109_117.htm DB - PRIME DP - Unbound Medicine ER -