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Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa.
Cell Calcium. 2014 Apr; 55(4):208-18.CC

Abstract

Merkel cells (MCs) have been proposed to form a part of the MC-neurite complex with sensory neurons. Many transient receptor potential (TRP) channels have been identified in mammals; however, the activation properties of these channels in oral mucosal MCs remain to be clarified. We investigated the biophysical and pharmacological properties of TRP vanilloid (TRPV)-1, TRPV2, TRPV4, TRP ankyrin (TRPA)-1, and TRP melastatin (TRPM)-8 channels, which are sensitive to osmotic and mechanical stimuli by measurement of intracellular free Ca(2+) concentration ([Ca(2+)]i) using fura-2. We also analyzed their localization patterns through immunofluorescence. MCs showed immunoreaction for TRPV1, TRPV2, TRPV4, TRPA1, and TRPM8 channels. In the presence of extracellular Ca(2+), the hypotonic test solution evoked Ca(2+) influx. The [Ca(2+)]i increases were inhibited by TRPV1, TRPV2, TRPV4, or TRPA1 channel antagonists, but not by the TRPM8 channel antagonist. Application of TRPV1, TRPV2, TRPV4, TRPA1, or TRPM8 channel selective agonists elicited transient increases in [Ca(2+)]i only in the presence of extracellular Ca(2+). The results indicate that membrane stretching in MCs activates TRPV1, TRPV2, TRPV4, and TRPA1 channels, that it may be involved in synaptic transmission to sensory neurons, and that MCs could contribute to the mechanosensory transduction sequence.

Authors+Show Affiliations

Department of Dental Anesthesiology, Tokyo Dental College, Chiba 261-8502, Japan.Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan.Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan.Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan.Department of Dental Anesthesiology, Tokyo Dental College, Chiba 261-8502, Japan.Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan.Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan. Electronic address: yshibuka@tdc.ac.jp.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24642224

Citation

Soya, Manabu, et al. "Plasma Membrane Stretch Activates Transient Receptor Potential Vanilloid and Ankyrin Channels in Merkel Cells From Hamster Buccal Mucosa." Cell Calcium, vol. 55, no. 4, 2014, pp. 208-18.
Soya M, Sato M, Sobhan U, et al. Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa. Cell Calcium. 2014;55(4):208-18.
Soya, M., Sato, M., Sobhan, U., Tsumura, M., Ichinohe, T., Tazaki, M., & Shibukawa, Y. (2014). Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa. Cell Calcium, 55(4), 208-18. https://doi.org/10.1016/j.ceca.2014.02.015
Soya M, et al. Plasma Membrane Stretch Activates Transient Receptor Potential Vanilloid and Ankyrin Channels in Merkel Cells From Hamster Buccal Mucosa. Cell Calcium. 2014;55(4):208-18. PubMed PMID: 24642224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Plasma membrane stretch activates transient receptor potential vanilloid and ankyrin channels in Merkel cells from hamster buccal mucosa. AU - Soya,Manabu, AU - Sato,Masaki, AU - Sobhan,Ubaidus, AU - Tsumura,Maki, AU - Ichinohe,Tatsuya, AU - Tazaki,Masakazu, AU - Shibukawa,Yoshiyuki, Y1 - 2014/02/28/ PY - 2013/10/17/received PY - 2014/02/05/revised PY - 2014/02/20/accepted PY - 2014/3/20/entrez PY - 2014/3/20/pubmed PY - 2014/12/15/medline KW - Mechanotransduction KW - Merkel cell KW - Sensory transduction KW - TRP SP - 208 EP - 18 JF - Cell calcium JO - Cell Calcium VL - 55 IS - 4 N2 - Merkel cells (MCs) have been proposed to form a part of the MC-neurite complex with sensory neurons. Many transient receptor potential (TRP) channels have been identified in mammals; however, the activation properties of these channels in oral mucosal MCs remain to be clarified. We investigated the biophysical and pharmacological properties of TRP vanilloid (TRPV)-1, TRPV2, TRPV4, TRP ankyrin (TRPA)-1, and TRP melastatin (TRPM)-8 channels, which are sensitive to osmotic and mechanical stimuli by measurement of intracellular free Ca(2+) concentration ([Ca(2+)]i) using fura-2. We also analyzed their localization patterns through immunofluorescence. MCs showed immunoreaction for TRPV1, TRPV2, TRPV4, TRPA1, and TRPM8 channels. In the presence of extracellular Ca(2+), the hypotonic test solution evoked Ca(2+) influx. The [Ca(2+)]i increases were inhibited by TRPV1, TRPV2, TRPV4, or TRPA1 channel antagonists, but not by the TRPM8 channel antagonist. Application of TRPV1, TRPV2, TRPV4, TRPA1, or TRPM8 channel selective agonists elicited transient increases in [Ca(2+)]i only in the presence of extracellular Ca(2+). The results indicate that membrane stretching in MCs activates TRPV1, TRPV2, TRPV4, and TRPA1 channels, that it may be involved in synaptic transmission to sensory neurons, and that MCs could contribute to the mechanosensory transduction sequence. SN - 1532-1991 UR - https://www.unboundmedicine.com/medline/citation/24642224/Plasma_membrane_stretch_activates_transient_receptor_potential_vanilloid_and_ankyrin_channels_in_Merkel_cells_from_hamster_buccal_mucosa_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4160(14)00037-2 DB - PRIME DP - Unbound Medicine ER -