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Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas.
Br J Cancer. 2014 Apr 29; 110(9):2246-9.BJ

Abstract

BACKGROUND

Kinase module of Mediator complex ('CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions.

METHODS

Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing.

RESULTS

None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13.

CONCLUSIONS

Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas.

Links

Publisher Full Text
ncbi.nlm.nih.gov
doi.org
PMC Free PDF

Authors+Show Affiliations

Mäkinen N
Department of Medical Genetics, Genome-Scale Biology Research Program, University of Helsinki, PO Box 63, 00014 Helsinki, Finland.
Heinonen HR
Department of Medical Genetics, Genome-Scale Biology Research Program, University of Helsinki, PO Box 63, 00014 Helsinki, Finland.
Sjöberg J
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, PO Box 140, 00029 Helsinki, Finland.
Taipale J
1] Genome-Scale Biology Research Program and Department of Pathology, Haartman Institute, University of Helsinki, PO Box 63, 00014 Helsinki, Finland [2] Science for Life Laboratory, Department of Biosciences and Nutrition, Karolinska Institute, Stockholm, Sweden.
Vahteristo P
Department of Medical Genetics, Genome-Scale Biology Research Program, University of Helsinki, PO Box 63, 00014 Helsinki, Finland.
Aaltonen LA
Department of Medical Genetics, Genome-Scale Biology Research Program, University of Helsinki, PO Box 63, 00014 Helsinki, Finland.

MeSH

Cyclin CCyclin-Dependent Kinase 8Cyclin-Dependent KinasesDNA Mutational AnalysisFemaleGenetic TestingHumansLeiomyomaMediator ComplexUterine Neoplasms

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24642626

Citation

Mäkinen, N, et al. "Mutation Analysis of Components of the Mediator Kinase Module in MED12 Mutation-negative Uterine Leiomyomas." British Journal of Cancer, vol. 110, no. 9, 2014, pp. 2246-9.
Mäkinen N, Heinonen HR, Sjöberg J, et al. Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas. Br J Cancer. 2014;110(9):2246-9.
Mäkinen, N., Heinonen, H. R., Sjöberg, J., Taipale, J., Vahteristo, P., & Aaltonen, L. A. (2014). Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas. British Journal of Cancer, 110(9), 2246-9. https://doi.org/10.1038/bjc.2014.138
Mäkinen N, et al. Mutation Analysis of Components of the Mediator Kinase Module in MED12 Mutation-negative Uterine Leiomyomas. Br J Cancer. 2014 Apr 29;110(9):2246-9. PubMed PMID: 24642626.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mutation analysis of components of the Mediator kinase module in MED12 mutation-negative uterine leiomyomas. AU - Mäkinen,N, AU - Heinonen,H-R, AU - Sjöberg,J, AU - Taipale,J, AU - Vahteristo,P, AU - Aaltonen,L A, Y1 - 2014/03/18/ PY - 2013/11/22/received PY - 2014/02/14/revised PY - 2014/02/21/accepted PY - 2014/3/20/entrez PY - 2014/3/20/pubmed PY - 2014/7/7/medline SP - 2246 EP - 9 JF - British journal of cancer JO - Br J Cancer VL - 110 IS - 9 N2 - BACKGROUND: Kinase module of Mediator complex ('CDK8 submodule') consists of four subunits: CDK8, Cyclin C, MED12, and MED13. Recently, we reported recurrent MED12 mutations in 70% of uterine leiomyomas. The aim of this study was to analyse whether mutations in other components of the module contribute to the development of these lesions. METHODS: Mutation screening of altogether 70 MED12 mutation-negative uterine leiomyomas was carried out by direct sequencing. RESULTS: None of the tumours displayed somatic mutations in the coding regions of CDK8/CDK19, CCNC, or MED13. CONCLUSIONS: Mutations in CDK8/CDK19, CCNC, and MED13 do not frequently contribute to genesis of uterine leiomyomas. SN - 1532-1827 UR - https://www.unboundmedicine.com/medline/citation/24642626/Mutation_analysis_of_components_of_the_Mediator_kinase_module_in_MED12_mutation_negative_uterine_leiomyomas_ L2 - https://doi.org/10.1038/bjc.2014.138 DB - PRIME DP - Unbound Medicine ER -