Tags

Type your tag names separated by a space and hit enter

Characterization of volatile constituents from Origanum onites and their antifungal and antibacterial activity.
J AOAC Int. 2013 Nov-Dec; 96(6):1200-8.JA

Abstract

Essential oils obtained by hydrodistillation (HD) and microwave-assisted HD (MWHD) of Origanum onites aerial parts were analyzed by GC and GCIMS. Thirty-one constituents representing 98.6% of the water-distilled oil and 52 constituents representing 99.6% of the microwave-distilled oil were identified. Carvacrol (76.8% HD and 79.2% MWHD) and thymol (4.7% HD and 4.4% MWHD) were characterized as major constituents in both essential oils. Separation of carvacrol and thymol was achieved by overpressured layer chromatography. HPTLC and TLC separations were also compared. Essential oils were evaluated for antifungal activity against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides using a direct overlay bioautography assay. Furthermore, main oil components carvacrol and thymol were then evaluated for antifungal activity; only carvacrol demonstrated nonselective antifungal activity against the three Colletotrichum species. Thymol and carvacrol were subsequently evaluated in a 96-well microdilution broth assay against Phomopsis obscurans, Fusarium oxysporum, three Colletotrichum species, and Botrytis cinerea. No activity was observed against any of the three Colletotrichum species at or below 30 pM. However, thymol demonstrated antifungal activity and produced 31.7% growth inhibition of P. obscurans at 120 h and 0.3 pM, whereas carvacrol appeared inactive. Thymol and carvacrol at 30 pM showed 51.5 and 36.9% growth inhibition of B. cinerea at 72 h. The mechanism of antibacterial activity was studied in a bioautography-based BioArena system. Thymol and carvacrol showed similar inhibition/killing effect against Bacillus subtilis soil bacteria; the action could be enhanced by the formaldehyde generator and transporter copper (II) ions and could be decreased in the presence of L-arginine, a formaldehyde capturer. Results indicated that Origanum essential oils and its major components thymol and carvacrol appear to generate antimicrobial activity through a mechanism of action where formaldehyde and its reaction products are produced.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24645494

Citation

Altintas, Ayhan, et al. "Characterization of Volatile Constituents From Origanum Onites and Their Antifungal and Antibacterial Activity." Journal of AOAC International, vol. 96, no. 6, 2013, pp. 1200-8.
Altintas A, Tabanca N, Tyihák E, et al. Characterization of volatile constituents from Origanum onites and their antifungal and antibacterial activity. J AOAC Int. 2013;96(6):1200-8.
Altintas, A., Tabanca, N., Tyihák, E., Ott, P. G., Móricz, A. M., Mincsovics, E., & Wedge, D. E. (2013). Characterization of volatile constituents from Origanum onites and their antifungal and antibacterial activity. Journal of AOAC International, 96(6), 1200-8.
Altintas A, et al. Characterization of Volatile Constituents From Origanum Onites and Their Antifungal and Antibacterial Activity. J AOAC Int. 2013 Nov-Dec;96(6):1200-8. PubMed PMID: 24645494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of volatile constituents from Origanum onites and their antifungal and antibacterial activity. AU - Altintas,Ayhan, AU - Tabanca,Nurhayat, AU - Tyihák,Erno, AU - Ott,Peter G, AU - Móricz,Agnes M, AU - Mincsovics,Emil, AU - Wedge,David E, PY - 2014/3/21/entrez PY - 2014/3/22/pubmed PY - 2014/4/20/medline SP - 1200 EP - 8 JF - Journal of AOAC International JO - J AOAC Int VL - 96 IS - 6 N2 - Essential oils obtained by hydrodistillation (HD) and microwave-assisted HD (MWHD) of Origanum onites aerial parts were analyzed by GC and GCIMS. Thirty-one constituents representing 98.6% of the water-distilled oil and 52 constituents representing 99.6% of the microwave-distilled oil were identified. Carvacrol (76.8% HD and 79.2% MWHD) and thymol (4.7% HD and 4.4% MWHD) were characterized as major constituents in both essential oils. Separation of carvacrol and thymol was achieved by overpressured layer chromatography. HPTLC and TLC separations were also compared. Essential oils were evaluated for antifungal activity against the strawberry anthracnose-causing fungal plant pathogens Colletotrichum acutatum, C. fragariae, and C. gloeosporioides using a direct overlay bioautography assay. Furthermore, main oil components carvacrol and thymol were then evaluated for antifungal activity; only carvacrol demonstrated nonselective antifungal activity against the three Colletotrichum species. Thymol and carvacrol were subsequently evaluated in a 96-well microdilution broth assay against Phomopsis obscurans, Fusarium oxysporum, three Colletotrichum species, and Botrytis cinerea. No activity was observed against any of the three Colletotrichum species at or below 30 pM. However, thymol demonstrated antifungal activity and produced 31.7% growth inhibition of P. obscurans at 120 h and 0.3 pM, whereas carvacrol appeared inactive. Thymol and carvacrol at 30 pM showed 51.5 and 36.9% growth inhibition of B. cinerea at 72 h. The mechanism of antibacterial activity was studied in a bioautography-based BioArena system. Thymol and carvacrol showed similar inhibition/killing effect against Bacillus subtilis soil bacteria; the action could be enhanced by the formaldehyde generator and transporter copper (II) ions and could be decreased in the presence of L-arginine, a formaldehyde capturer. Results indicated that Origanum essential oils and its major components thymol and carvacrol appear to generate antimicrobial activity through a mechanism of action where formaldehyde and its reaction products are produced. SN - 1060-3271 UR - https://www.unboundmedicine.com/medline/citation/24645494/Characterization_of_volatile_constituents_from_Origanum_onites_and_their_antifungal_and_antibacterial_activity_ L2 - https://medlineplus.gov/antibiotics.html DB - PRIME DP - Unbound Medicine ER -