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The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae.
FEMS Yeast Res. 2014 Jun; 14(4):642-53.FY

Abstract

High acetic acid tolerance of Saccharomyces cerevisiae is a relevant phenotype in industrial biotechnology when using lignocellulosic hydrolysates as feedstock. A screening of 38 S. cerevisiae strains for tolerance to acetic acid revealed considerable differences, particularly with regard to the duration of the latency phase. To understand how this phenotype is quantitatively manifested, four strains exhibiting significant differences were studied in more detail. Our data show that the duration of the latency phase is primarily determined by the fraction of cells within the population that resume growth. Only this fraction contributed to the exponential growth observed after the latency phase, while all other cells persisted in a viable but non-proliferating state. A remarkable variation in the size of the fraction was observed among the tested strains differing by several orders of magnitude. In fact, only 11 out of 10(7) cells of the industrial bioethanol production strain Ethanol Red resumed growth after exposure to 157 mM acetic acid at pH 4.5, while this fraction was 3.6 × 10(6) (out of 10(7) cells) in the highly acetic acid tolerant isolate ATCC 96581. These strain-specific differences are genetically determined and represent a valuable starting point to identify genetic targets for future strain improvement.

Authors+Show Affiliations

School of Engineering and Science, Jacobs University gGmbH, Bremen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24645649

Citation

Swinnen, Steve, et al. "The Fraction of Cells That Resume Growth After Acetic Acid Addition Is a Strain-dependent Parameter of Acetic Acid Tolerance in Saccharomyces Cerevisiae." FEMS Yeast Research, vol. 14, no. 4, 2014, pp. 642-53.
Swinnen S, Fernández-Niño M, González-Ramos D, et al. The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae. FEMS Yeast Res. 2014;14(4):642-53.
Swinnen, S., Fernández-Niño, M., González-Ramos, D., van Maris, A. J., & Nevoigt, E. (2014). The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae. FEMS Yeast Research, 14(4), 642-53. https://doi.org/10.1111/1567-1364.12151
Swinnen S, et al. The Fraction of Cells That Resume Growth After Acetic Acid Addition Is a Strain-dependent Parameter of Acetic Acid Tolerance in Saccharomyces Cerevisiae. FEMS Yeast Res. 2014;14(4):642-53. PubMed PMID: 24645649.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The fraction of cells that resume growth after acetic acid addition is a strain-dependent parameter of acetic acid tolerance in Saccharomyces cerevisiae. AU - Swinnen,Steve, AU - Fernández-Niño,Miguel, AU - González-Ramos,Daniel, AU - van Maris,Antonius J A, AU - Nevoigt,Elke, Y1 - 2014/04/11/ PY - 2013/12/09/received PY - 2014/03/12/revised PY - 2014/03/12/accepted PY - 2014/3/21/entrez PY - 2014/3/22/pubmed PY - 2015/1/21/medline KW - Saccharomyces cerevisiae KW - acetic acid tolerance KW - cell-to-cell heterogeneity KW - intraspecies diversity KW - lignocellulose KW - yeast SP - 642 EP - 53 JF - FEMS yeast research JO - FEMS Yeast Res VL - 14 IS - 4 N2 - High acetic acid tolerance of Saccharomyces cerevisiae is a relevant phenotype in industrial biotechnology when using lignocellulosic hydrolysates as feedstock. A screening of 38 S. cerevisiae strains for tolerance to acetic acid revealed considerable differences, particularly with regard to the duration of the latency phase. To understand how this phenotype is quantitatively manifested, four strains exhibiting significant differences were studied in more detail. Our data show that the duration of the latency phase is primarily determined by the fraction of cells within the population that resume growth. Only this fraction contributed to the exponential growth observed after the latency phase, while all other cells persisted in a viable but non-proliferating state. A remarkable variation in the size of the fraction was observed among the tested strains differing by several orders of magnitude. In fact, only 11 out of 10(7) cells of the industrial bioethanol production strain Ethanol Red resumed growth after exposure to 157 mM acetic acid at pH 4.5, while this fraction was 3.6 × 10(6) (out of 10(7) cells) in the highly acetic acid tolerant isolate ATCC 96581. These strain-specific differences are genetically determined and represent a valuable starting point to identify genetic targets for future strain improvement. SN - 1567-1364 UR - https://www.unboundmedicine.com/medline/citation/24645649/The_fraction_of_cells_that_resume_growth_after_acetic_acid_addition_is_a_strain_dependent_parameter_of_acetic_acid_tolerance_in_Saccharomyces_cerevisiae_ L2 - https://academic.oup.com/femsyr/article-lookup/doi/10.1111/1567-1364.12151 DB - PRIME DP - Unbound Medicine ER -