Tags

Type your tag names separated by a space and hit enter

Signal-on dual-potential electrochemiluminescence based on luminol-gold bifunctional nanoparticles for telomerase detection.
Anal Chem. 2014 Apr 15; 86(8):3834-40.AC

Abstract

Here, we report a novel type of signal-on dual-potential electrochemiluminescence (ECL) approach for telomerase detection based on bifunctionalized luminol-gold nanoparticles (L-Au NPs). In this approach, CdS nanocrystals (NCs) were first coated on glassy carbon electrode, and then thiol-modified telomerase primer was attached on CdS NCs via Cd-S bond. In the presence of telomerase and dNTPs, the primer could be extended. Telomerase primer would hybridize with its complementary DNA, and the extended part would hybridize with the capture DNA which was tagged with L-Au NPs. In the presence of coreactant H2O2, the L-Au NPs could not only enhance the ECL intensity of CdS NCs at -1.25 V (vs SCE) induced by the surface plasmon resonance (SPR) of Au NPs but also produce a new ECL signal at +0.45 V (vs SCE) that resulted from luminol in L-Au NPs. Both signals at two potentials increased with the increase of telomerase concentration. This method could be used to detect the telomerase from 100 to 9000 HL-60 cells and investigate the apoptosis of tumor cells. The ratio of the two signal increments (ΔECL(Luminol)/ΔECL(CdS NCs)), which showed a high consistency value for different numbers of cells, could be used to verify the reliability of tests. This dual-potential ECL strategy showed great promise in avoiding false positive or negative results in bioanalysis.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang HR
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210093, China.
Wu MS
No affiliation info available
Xu JJ
No affiliation info available
Chen HY
No affiliation info available

MeSH

ApoptosisAptamers, NucleotideCadmium CompoundsDNAElectrochemical TechniquesGoldHL-60 CellsHumansLuminescent MeasurementsLuminolMetal NanoparticlesSulfidesSurface Plasmon ResonanceTelomerase

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24646287

Citation

Zhang, Huai-Rong, et al. "Signal-on Dual-potential Electrochemiluminescence Based On Luminol-gold Bifunctional Nanoparticles for Telomerase Detection." Analytical Chemistry, vol. 86, no. 8, 2014, pp. 3834-40.
Zhang HR, Wu MS, Xu JJ, et al. Signal-on dual-potential electrochemiluminescence based on luminol-gold bifunctional nanoparticles for telomerase detection. Anal Chem. 2014;86(8):3834-40.
Zhang, H. R., Wu, M. S., Xu, J. J., & Chen, H. Y. (2014). Signal-on dual-potential electrochemiluminescence based on luminol-gold bifunctional nanoparticles for telomerase detection. Analytical Chemistry, 86(8), 3834-40. https://doi.org/10.1021/ac403960g
Zhang HR, et al. Signal-on Dual-potential Electrochemiluminescence Based On Luminol-gold Bifunctional Nanoparticles for Telomerase Detection. Anal Chem. 2014 Apr 15;86(8):3834-40. PubMed PMID: 24646287.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Signal-on dual-potential electrochemiluminescence based on luminol-gold bifunctional nanoparticles for telomerase detection. AU - Zhang,Huai-Rong, AU - Wu,Mei-Sheng, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2014/03/27/ PY - 2014/3/21/entrez PY - 2014/3/22/pubmed PY - 2015/7/2/medline SP - 3834 EP - 40 JF - Analytical chemistry JO - Anal Chem VL - 86 IS - 8 N2 - Here, we report a novel type of signal-on dual-potential electrochemiluminescence (ECL) approach for telomerase detection based on bifunctionalized luminol-gold nanoparticles (L-Au NPs). In this approach, CdS nanocrystals (NCs) were first coated on glassy carbon electrode, and then thiol-modified telomerase primer was attached on CdS NCs via Cd-S bond. In the presence of telomerase and dNTPs, the primer could be extended. Telomerase primer would hybridize with its complementary DNA, and the extended part would hybridize with the capture DNA which was tagged with L-Au NPs. In the presence of coreactant H2O2, the L-Au NPs could not only enhance the ECL intensity of CdS NCs at -1.25 V (vs SCE) induced by the surface plasmon resonance (SPR) of Au NPs but also produce a new ECL signal at +0.45 V (vs SCE) that resulted from luminol in L-Au NPs. Both signals at two potentials increased with the increase of telomerase concentration. This method could be used to detect the telomerase from 100 to 9000 HL-60 cells and investigate the apoptosis of tumor cells. The ratio of the two signal increments (ΔECL(Luminol)/ΔECL(CdS NCs)), which showed a high consistency value for different numbers of cells, could be used to verify the reliability of tests. This dual-potential ECL strategy showed great promise in avoiding false positive or negative results in bioanalysis. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/24646287/Signal_on_dual_potential_electrochemiluminescence_based_on_luminol_gold_bifunctional_nanoparticles_for_telomerase_detection_ DB - PRIME DP - Unbound Medicine ER -