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Antioxidant and hepatoprotective effects of Solanum xanthocarpum leaf extracts against CCl4-induced liver injury in rats.
Pharm Biol. 2014 Aug; 52(8):1060-8.PB

Abstract

CONTEXT

Solanum xanthocarpum Schard. and Wendl. (Solanaceae) has been used in traditional Indian medicines for its antioxidant, anti-inflammatory, and antiasthmatic properties.

OBJECTIVE

The present study demonstrates the antioxidant and hepatoprotective effects of S. xanthocarpum. On the basis of in vitro antioxidant properties, the active fraction from column chromatography of the methanol extract of S. xanthocarpum leaves (SXAF) was chosen as the potent fraction and used for hepatoprotective studies in rats.

MATERIALS AND METHODS

The antioxidant activity was evaluated by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and reducing power assays. Rats were pre-treated with 100 and 200 mg/kg b.w. of SXAF for 14 d with a single dose of CCl4 in the last day. Hepatoprotective properties were determined by serum biochemical enzymes, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), antioxidant enzymes (SOD, CAT, GSH, and GST), and histopathology studies.

RESULTS

SXAF exhibited significant antioxidant activity in scavenging free radicals with IC50 values of 11.72 µg (DPPH) and 17.99 µg (ABTS). Rats pre-treated with SXAF demonstrated significantly reduced levels of serum LDH (1.7-fold), ALP (1.6-fold), and AST (1.8-fold). Similarly, multiple dose SXAF administration at 200 mg/kg b.w. demonstrated significantly enhanced levels of SOD (1.78 ± 0.13), CAT (34.63 ± 1.98), GST (231.64 ± 14.28), and GSH (8.23 ± 0.48) in liver homogenates. Histopathological examination showed lowered liver damage in SXAF-treated groups.

DISCUSSION AND CONCLUSION

These results demonstrate that SXAF possesses potent antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity.

Authors+Show Affiliations

Department of Studies in Biotechnology, University of Mysore , Manasagangotri, Mysore, Karnataka , India.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24646306

Citation

Jalali Ghassam, Behrouz, et al. "Antioxidant and Hepatoprotective Effects of Solanum Xanthocarpum Leaf Extracts Against CCl4-induced Liver Injury in Rats." Pharmaceutical Biology, vol. 52, no. 8, 2014, pp. 1060-8.
Jalali Ghassam B, Ghaffari H, Prakash HS, et al. Antioxidant and hepatoprotective effects of Solanum xanthocarpum leaf extracts against CCl4-induced liver injury in rats. Pharm Biol. 2014;52(8):1060-8.
Jalali Ghassam, B., Ghaffari, H., Prakash, H. S., & Kini, K. R. (2014). Antioxidant and hepatoprotective effects of Solanum xanthocarpum leaf extracts against CCl4-induced liver injury in rats. Pharmaceutical Biology, 52(8), 1060-8. https://doi.org/10.3109/13880209.2013.877490
Jalali Ghassam B, et al. Antioxidant and Hepatoprotective Effects of Solanum Xanthocarpum Leaf Extracts Against CCl4-induced Liver Injury in Rats. Pharm Biol. 2014;52(8):1060-8. PubMed PMID: 24646306.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant and hepatoprotective effects of Solanum xanthocarpum leaf extracts against CCl4-induced liver injury in rats. AU - Jalali Ghassam,Behrouz, AU - Ghaffari,Hadi, AU - Prakash,H S, AU - Kini,Kukkundoor Ramachandra, Y1 - 2014/03/19/ PY - 2014/3/21/entrez PY - 2014/3/22/pubmed PY - 2015/3/31/medline KW - DPPH KW - free radicals KW - histopathology KW - lipid peroxidation KW - oxidative stress KW - phenolic compounds KW - superoxide dismutase SP - 1060 EP - 8 JF - Pharmaceutical biology JO - Pharm Biol VL - 52 IS - 8 N2 - CONTEXT: Solanum xanthocarpum Schard. and Wendl. (Solanaceae) has been used in traditional Indian medicines for its antioxidant, anti-inflammatory, and antiasthmatic properties. OBJECTIVE: The present study demonstrates the antioxidant and hepatoprotective effects of S. xanthocarpum. On the basis of in vitro antioxidant properties, the active fraction from column chromatography of the methanol extract of S. xanthocarpum leaves (SXAF) was chosen as the potent fraction and used for hepatoprotective studies in rats. MATERIALS AND METHODS: The antioxidant activity was evaluated by 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and reducing power assays. Rats were pre-treated with 100 and 200 mg/kg b.w. of SXAF for 14 d with a single dose of CCl4 in the last day. Hepatoprotective properties were determined by serum biochemical enzymes, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), antioxidant enzymes (SOD, CAT, GSH, and GST), and histopathology studies. RESULTS: SXAF exhibited significant antioxidant activity in scavenging free radicals with IC50 values of 11.72 µg (DPPH) and 17.99 µg (ABTS). Rats pre-treated with SXAF demonstrated significantly reduced levels of serum LDH (1.7-fold), ALP (1.6-fold), and AST (1.8-fold). Similarly, multiple dose SXAF administration at 200 mg/kg b.w. demonstrated significantly enhanced levels of SOD (1.78 ± 0.13), CAT (34.63 ± 1.98), GST (231.64 ± 14.28), and GSH (8.23 ± 0.48) in liver homogenates. Histopathological examination showed lowered liver damage in SXAF-treated groups. DISCUSSION AND CONCLUSION: These results demonstrate that SXAF possesses potent antioxidant properties as well as hepatoprotective effects against CCl4-induced hepatotoxicity. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/24646306/Antioxidant_and_hepatoprotective_effects_of_Solanum_xanthocarpum_leaf_extracts_against_CCl4_induced_liver_injury_in_rats_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2013.877490 DB - PRIME DP - Unbound Medicine ER -