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Identification of blood serum micro-RNAs associated with idiopathic and LRRK2 Parkinson's disease.
J Neurosci Res. 2014 Aug; 92(8):1071-7.JN

Abstract

Blood-cell-free circulating micro-RNAs (miRNAs) have been proposed as potential accessible biomarkers for neurodegenerative diseases such as Parkinson's disease (PD). Here we analyzed the serum levels of 377 miRNAs in a discovery set of 10 idiopathic Parkinson's disease (IPD) patients, 10 PD patients carriers of the LRRK2 G2019S mutation (LRRK2 PD), and 10 controls by using real-time quantitative PCR-based TaqMan MicroRNA arrays. We detected candidate differentially expressed miRNAs, which were further tested in a first validation set consisting of 20 IPD, 20 LRRK2 PD, and 20 control samples. We found four statistically significant miRNAs that were downregulated in either LRRK2 or IPD (miR-29a, miR-29c, miR-19a, and miR-19b). Subsequently, we validated these findings in a third set of samples consisting of 65 IPD and 65 controls and confirmed the association of downregulated levels of miR-29c, miR-29a, and miR-19b in IPD. Differentially expressed miRNAs are predicted to target genes belonging to pathways related to ECM-receptor interaction, focal adhesion, MAPK, Wnt, mTOR, adipocytokine, and neuron projection. Results from our exploratory study indicate that downregulated levels of specific circulating serum miRNAs are associated with PD and suggest their potential use as noninvasive biomarkers for PD. Future studies should further confirm the association of these miRNAs with PD.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Botta-Orfila T
Laboratory of Neurodegenerative Disorders, Department of Neurology, Hospital Clínic-Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS)-Centro de Investigación sobre Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Catalonia, Spain.
Morató X
No affiliation info available
Compta Y
No affiliation info available
Lozano JJ
No affiliation info available
Falgàs N
No affiliation info available
Valldeoriola F
No affiliation info available
Pont-Sunyer C
No affiliation info available
Vilas D
No affiliation info available
Mengual L
No affiliation info available
Fernández M
No affiliation info available
Molinuevo JL
No affiliation info available
Antonell A
No affiliation info available
Martí MJ
No affiliation info available
Fernández-Santiago R
No affiliation info available
Ezquerra M
No affiliation info available

MeSH

AdultAgedFemaleGene Expression ProfilingHumansLeucine-Rich Repeat Serine-Threonine Protein Kinase-2MaleMicroRNAsMiddle AgedParkinson DiseasePilot ProjectsProtein Serine-Threonine Kinases

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24648008

Citation

Botta-Orfila, Teresa, et al. "Identification of Blood Serum micro-RNAs Associated With Idiopathic and LRRK2 Parkinson's Disease." Journal of Neuroscience Research, vol. 92, no. 8, 2014, pp. 1071-7.
Botta-Orfila T, Morató X, Compta Y, et al. Identification of blood serum micro-RNAs associated with idiopathic and LRRK2 Parkinson's disease. J Neurosci Res. 2014;92(8):1071-7.
Botta-Orfila, T., Morató, X., Compta, Y., Lozano, J. J., Falgàs, N., Valldeoriola, F., Pont-Sunyer, C., Vilas, D., Mengual, L., Fernández, M., Molinuevo, J. L., Antonell, A., Martí, M. J., Fernández-Santiago, R., & Ezquerra, M. (2014). Identification of blood serum micro-RNAs associated with idiopathic and LRRK2 Parkinson's disease. Journal of Neuroscience Research, 92(8), 1071-7. https://doi.org/10.1002/jnr.23377
Botta-Orfila T, et al. Identification of Blood Serum micro-RNAs Associated With Idiopathic and LRRK2 Parkinson's Disease. J Neurosci Res. 2014;92(8):1071-7. PubMed PMID: 24648008.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of blood serum micro-RNAs associated with idiopathic and LRRK2 Parkinson's disease. AU - Botta-Orfila,Teresa, AU - Morató,Xavier, AU - Compta,Yaroslau, AU - Lozano,Juan José, AU - Falgàs,Neus, AU - Valldeoriola,Francesc, AU - Pont-Sunyer,Claustre, AU - Vilas,Dolores, AU - Mengual,Lourdes, AU - Fernández,Manel, AU - Molinuevo,José Luis, AU - Antonell,Anna, AU - Martí,Maria José, AU - Fernández-Santiago,Rubén, AU - Ezquerra,Mario, Y1 - 2014/03/20/ PY - 2013/08/06/received PY - 2014/01/21/revised PY - 2014/02/12/accepted PY - 2014/3/21/entrez PY - 2014/3/22/pubmed PY - 2015/2/24/medline KW - Parkinson's disease KW - biomarker KW - blood KW - miRNAs SP - 1071 EP - 7 JF - Journal of neuroscience research JO - J Neurosci Res VL - 92 IS - 8 N2 - Blood-cell-free circulating micro-RNAs (miRNAs) have been proposed as potential accessible biomarkers for neurodegenerative diseases such as Parkinson's disease (PD). Here we analyzed the serum levels of 377 miRNAs in a discovery set of 10 idiopathic Parkinson's disease (IPD) patients, 10 PD patients carriers of the LRRK2 G2019S mutation (LRRK2 PD), and 10 controls by using real-time quantitative PCR-based TaqMan MicroRNA arrays. We detected candidate differentially expressed miRNAs, which were further tested in a first validation set consisting of 20 IPD, 20 LRRK2 PD, and 20 control samples. We found four statistically significant miRNAs that were downregulated in either LRRK2 or IPD (miR-29a, miR-29c, miR-19a, and miR-19b). Subsequently, we validated these findings in a third set of samples consisting of 65 IPD and 65 controls and confirmed the association of downregulated levels of miR-29c, miR-29a, and miR-19b in IPD. Differentially expressed miRNAs are predicted to target genes belonging to pathways related to ECM-receptor interaction, focal adhesion, MAPK, Wnt, mTOR, adipocytokine, and neuron projection. Results from our exploratory study indicate that downregulated levels of specific circulating serum miRNAs are associated with PD and suggest their potential use as noninvasive biomarkers for PD. Future studies should further confirm the association of these miRNAs with PD. SN - 1097-4547 UR - https://www.unboundmedicine.com/medline/citation/24648008/Identification_of_blood_serum_micro_RNAs_associated_with_idiopathic_and_LRRK2_Parkinson's_disease_ DB - PRIME DP - Unbound Medicine ER -