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Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis.
Anal Chem 2014; 86(8):4041-6AC

Abstract

A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined. The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt. The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition. It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h. Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse. The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis.

Authors+Show Affiliations

Department of Chemistry, University of Michigan , 930 N. University, Ann Arbor, MI 48109-1055, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24650129

Citation

Bell-Vlasov, Andrea K., et al. "Polyion Selective Polymeric Membrane-based Pulstrode as a Detector in Flow-injection Analysis." Analytical Chemistry, vol. 86, no. 8, 2014, pp. 4041-6.
Bell-Vlasov AK, Zajda J, Eldourghamy A, et al. Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis. Anal Chem. 2014;86(8):4041-6.
Bell-Vlasov, A. K., Zajda, J., Eldourghamy, A., Malinowska, E., & Meyerhoff, M. E. (2014). Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis. Analytical Chemistry, 86(8), pp. 4041-6. doi:10.1021/ac500567g.
Bell-Vlasov AK, et al. Polyion Selective Polymeric Membrane-based Pulstrode as a Detector in Flow-injection Analysis. Anal Chem. 2014 Apr 15;86(8):4041-6. PubMed PMID: 24650129.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyion selective polymeric membrane-based pulstrode as a detector in flow-injection analysis. AU - Bell-Vlasov,Andrea K, AU - Zajda,Joanna, AU - Eldourghamy,Ayman, AU - Malinowska,Elzbieta, AU - Meyerhoff,Mark E, Y1 - 2014/04/01/ PY - 2014/3/22/entrez PY - 2014/3/22/pubmed PY - 2015/6/25/medline SP - 4041 EP - 6 JF - Analytical chemistry JO - Anal. Chem. VL - 86 IS - 8 N2 - A method for the detection of polyions using fully reversible polyion selective polymeric membrane type pulstrodes as detectors in a flow-injection analysis (FIA) system is examined. The detection electrode consists of a plasticized polymeric membrane doped with 10 wt % of tridodecylmethylammonium-dinonylnaphthalene sulfonate (TDMA/DNNS) ion-exchanger salt. The pulse sequence used involves a short (1 s) galvanostatic pulse, an open-circuit pulse (0.5 s) during which the EMF of the cell is measured, and a longer (15 s) potentiostatic pulse to return the membrane to its original chemical composition. It is shown that total pulse sequence times can be optimized to yield reproducible real-time detection of injected samples of protamine and heparin at up to 20 samples/h. Further, it is shown that the same membrane detector can be employed for FIA detection of both polycations at levels ≥10 μg/mL and polyanions at levels of ≥40 μg/mL by changing the direction of the galvanostatic pulse. The methodology described may also be applicable in the detection of polyionic species at low levels in other flowing configurations, such as in liquid chromatography and capillary electrophoresis. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/24650129/Polyion_selective_polymeric_membrane_based_pulstrode_as_a_detector_in_flow_injection_analysis_ L2 - https://dx.doi.org/10.1021/ac500567g DB - PRIME DP - Unbound Medicine ER -