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Cannabinoid disposition in oral fluid after controlled cannabis smoking in frequent and occasional smokers.
Drug Test Anal. 2014 Oct; 6(10):1002-10.DT

Abstract

Oral fluid (OF) is an increasingly popular alternative matrix for drug testing, with cannabinoids being the most commonly identified illicit drug. Quantification of multiple OF cannabinoids and understanding differences in OF cannabinoid pharmacokinetics between frequent and occasional smokers improve test interpretation. The new Oral-Eze® OF collection device has an elution buffer that stabilizes analytes and improves drug recovery from the collection pad; however, its performance has not been independently evaluated. After controlled smoking of a 6.8% Δ(9) -tetrahydrocannabinol (THC) cannabis cigarette by frequent and occasional smokers, OF was collected with the Oral-Eze device for up to 30 h. Samples were analyzed for multiple cannabinoids by a validated 2D-GC-MS method. Frequent smokers had significantly greater OF THCCOOH concentrations than occasional smokers at all times, and showed positive results for a significantly longer time. We evaluated multiple cannabinoid cut-offs; the shortest last detection times were observed when THC ≥ 1 μg/L was combined with CBD or CBN ≥ 1 μg/L. With these cut-offs, last detection times(1-13.5 h) were not significantly different between groups, demonstrating suitability for short-term cannabinoid detection independent of smoking history. Cut-offs utilizing THC alone or combined with THCCOOH showed significantly different last detection times between groups. The widest detection windows were observed with THC ≥ 1 or 2 μg/L or THCCOOH ≥ 20 ng/L. Our data illustrate the effectiveness of the Oral-Eze® device for OF collection, the impact of self-administered smoked cannabis history on OF cannabinoid results, and the ability to improve interpretation and tailor OF cannabinoid cut-offs to fulfill the detection window needs of a given program.

Authors+Show Affiliations

Chemistry and Drug Metabolism Section, IRP, National Institute on Drug Abuse, National Institutes of Health, Baltimore, MD, USA; Program in Toxicology, University of Maryland Baltimore, Baltimore, MD, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

24652685

Citation

Newmeyer, Matthew N., et al. "Cannabinoid Disposition in Oral Fluid After Controlled Cannabis Smoking in Frequent and Occasional Smokers." Drug Testing and Analysis, vol. 6, no. 10, 2014, pp. 1002-10.
Newmeyer MN, Desrosiers NA, Lee D, et al. Cannabinoid disposition in oral fluid after controlled cannabis smoking in frequent and occasional smokers. Drug Test Anal. 2014;6(10):1002-10.
Newmeyer, M. N., Desrosiers, N. A., Lee, D., Mendu, D. R., Barnes, A. J., Gorelick, D. A., & Huestis, M. A. (2014). Cannabinoid disposition in oral fluid after controlled cannabis smoking in frequent and occasional smokers. Drug Testing and Analysis, 6(10), 1002-10. https://doi.org/10.1002/dta.1632
Newmeyer MN, et al. Cannabinoid Disposition in Oral Fluid After Controlled Cannabis Smoking in Frequent and Occasional Smokers. Drug Test Anal. 2014;6(10):1002-10. PubMed PMID: 24652685.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid disposition in oral fluid after controlled cannabis smoking in frequent and occasional smokers. AU - Newmeyer,Matthew N, AU - Desrosiers,Nathalie A, AU - Lee,Dayong, AU - Mendu,Damodara R, AU - Barnes,Allan J, AU - Gorelick,David A, AU - Huestis,Marilyn A, Y1 - 2014/03/20/ PY - 2013/12/20/received PY - 2014/01/24/revised PY - 2014/02/08/accepted PY - 2014/3/22/entrez PY - 2014/3/22/pubmed PY - 2015/6/20/medline KW - Oral-Eze® KW - cannabinoids KW - oral fluid SP - 1002 EP - 10 JF - Drug testing and analysis JO - Drug Test Anal VL - 6 IS - 10 N2 - Oral fluid (OF) is an increasingly popular alternative matrix for drug testing, with cannabinoids being the most commonly identified illicit drug. Quantification of multiple OF cannabinoids and understanding differences in OF cannabinoid pharmacokinetics between frequent and occasional smokers improve test interpretation. The new Oral-Eze® OF collection device has an elution buffer that stabilizes analytes and improves drug recovery from the collection pad; however, its performance has not been independently evaluated. After controlled smoking of a 6.8% Δ(9) -tetrahydrocannabinol (THC) cannabis cigarette by frequent and occasional smokers, OF was collected with the Oral-Eze device for up to 30 h. Samples were analyzed for multiple cannabinoids by a validated 2D-GC-MS method. Frequent smokers had significantly greater OF THCCOOH concentrations than occasional smokers at all times, and showed positive results for a significantly longer time. We evaluated multiple cannabinoid cut-offs; the shortest last detection times were observed when THC ≥ 1 μg/L was combined with CBD or CBN ≥ 1 μg/L. With these cut-offs, last detection times(1-13.5 h) were not significantly different between groups, demonstrating suitability for short-term cannabinoid detection independent of smoking history. Cut-offs utilizing THC alone or combined with THCCOOH showed significantly different last detection times between groups. The widest detection windows were observed with THC ≥ 1 or 2 μg/L or THCCOOH ≥ 20 ng/L. Our data illustrate the effectiveness of the Oral-Eze® device for OF collection, the impact of self-administered smoked cannabis history on OF cannabinoid results, and the ability to improve interpretation and tailor OF cannabinoid cut-offs to fulfill the detection window needs of a given program. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/24652685/Cannabinoid_disposition_in_oral_fluid_after_controlled_cannabis_smoking_in_frequent_and_occasional_smokers_ L2 - https://doi.org/10.1002/dta.1632 DB - PRIME DP - Unbound Medicine ER -