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Cytoprotective and antioxidant effects of the methanol extract of Eremomastax speciosa in rats.
Afr J Tradit Complement Altern Med. 2014; 11(1):165-71.AJ

Abstract

BACKGROUND

Ethno-botanical information shows that Eremomastax speciosa is used in the traditional management of various stomach complaints including gastro-duodenal ulcers.

MATERIALS AND METHODS

In this study, we tested the cytoprotective potential of the whole plant methanol extract (100-200 mg/kg, p.o), against HCl/ethanol, absolute ethanol, cold/restraint stress rats, and pylorus legated rats pre-treated with indomethacin. The effects of the extract on gastric lesion inhibition, the volume of gastric juice, gastric pH, gastric acid output, mucus production and gastric peptic activity were recorded. Oxidative stress parameters were measured in blood and gastric tissue samples obtained from the animals in all the models tested.

RESULTS

The extract significantly (p<0.05), reduced the formation of cold/restraint ulcers by (31-60%, inhibition), completely inhibited (100%) the formation of lesions induced by HCl/ethanol at the highest dose, but was less effective against absolute ethanol (22-46% inhibition). The extract (200 mg/kg), significantly reduced lesion formation (P<0.01), gastric acidity (P<0.01), and volume of gastric secretions (P<0.05), in the indomethacin/pylorus ligation model, and did not affect the activity of pepsin in gastric juice. Blood concentrations of antioxidant enzymes (catalase, SOD and GSH), increased significantly and MDA concentrations decreased in all models tested.

CONCLUSION

Cytoprotection by E. speciosa methanol extract was attributed to its ability to reduce acid secretion, and to enhance mucosal defence and in vivo antioxidant status.

Authors+Show Affiliations

Department of Animal Biology and Physiology, Faculty of Science, P.O. Box 812, University of Yaounde I, Cameroon.Department of Animal Biology and Physiology, Faculty of Science, P.O. Box 812, University of Yaounde I, Cameroon.Department of Animal Biology and Physiology, Faculty of Science, P.O. Box 812, University of Yaounde I, Cameroon.Department of Animal Biology and Physiology, Faculty of Science, P.O. Box 812, University of Yaounde I, Cameroon.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24653572

Citation

Amang, Perfusion A., et al. "Cytoprotective and Antioxidant Effects of the Methanol Extract of Eremomastax Speciosa in Rats." African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, vol. 11, no. 1, 2014, pp. 165-71.
Amang PA, Tan PV, Patamaken SA, et al. Cytoprotective and antioxidant effects of the methanol extract of Eremomastax speciosa in rats. Afr J Tradit Complement Altern Med. 2014;11(1):165-71.
Amang, P. A., Tan, P. V., Patamaken, S. A., & Mefe, M. N. (2014). Cytoprotective and antioxidant effects of the methanol extract of Eremomastax speciosa in rats. African Journal of Traditional, Complementary, and Alternative Medicines : AJTCAM, 11(1), 165-71.
Amang PA, et al. Cytoprotective and Antioxidant Effects of the Methanol Extract of Eremomastax Speciosa in Rats. Afr J Tradit Complement Altern Med. 2014;11(1):165-71. PubMed PMID: 24653572.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cytoprotective and antioxidant effects of the methanol extract of Eremomastax speciosa in rats. AU - Amang,Perfusion A, AU - Tan,Paul V, AU - Patamaken,Simon A, AU - Mefe,Martial N, Y1 - 2013/11/02/ PY - 2014/3/22/entrez PY - 2014/3/22/pubmed PY - 2014/11/13/medline KW - Eremomastax speciosa KW - antioxidant status KW - cytoprotection KW - gastric ulcers SP - 165 EP - 71 JF - African journal of traditional, complementary, and alternative medicines : AJTCAM JO - Afr J Tradit Complement Altern Med VL - 11 IS - 1 N2 - BACKGROUND: Ethno-botanical information shows that Eremomastax speciosa is used in the traditional management of various stomach complaints including gastro-duodenal ulcers. MATERIALS AND METHODS: In this study, we tested the cytoprotective potential of the whole plant methanol extract (100-200 mg/kg, p.o), against HCl/ethanol, absolute ethanol, cold/restraint stress rats, and pylorus legated rats pre-treated with indomethacin. The effects of the extract on gastric lesion inhibition, the volume of gastric juice, gastric pH, gastric acid output, mucus production and gastric peptic activity were recorded. Oxidative stress parameters were measured in blood and gastric tissue samples obtained from the animals in all the models tested. RESULTS: The extract significantly (p<0.05), reduced the formation of cold/restraint ulcers by (31-60%, inhibition), completely inhibited (100%) the formation of lesions induced by HCl/ethanol at the highest dose, but was less effective against absolute ethanol (22-46% inhibition). The extract (200 mg/kg), significantly reduced lesion formation (P<0.01), gastric acidity (P<0.01), and volume of gastric secretions (P<0.05), in the indomethacin/pylorus ligation model, and did not affect the activity of pepsin in gastric juice. Blood concentrations of antioxidant enzymes (catalase, SOD and GSH), increased significantly and MDA concentrations decreased in all models tested. CONCLUSION: Cytoprotection by E. speciosa methanol extract was attributed to its ability to reduce acid secretion, and to enhance mucosal defence and in vivo antioxidant status. SN - 2505-0044 UR - https://www.unboundmedicine.com/medline/citation/24653572/Cytoprotective_and_antioxidant_effects_of_the_methanol_extract_of_Eremomastax_speciosa_in_rats_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24653572/ DB - PRIME DP - Unbound Medicine ER -