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Porphyromonas gingivalis gingipain is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm.
Mol Oral Microbiol. 2014 Jun; 29(3):131-43.MO

Abstract

Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are major periodontal pathogens that cause several types of periodontal disease. Our previous study suggested that P. gingivalis gingipains secreted in the subgingival environment are related to the detachment of A.actinomycetemcomitans biofilms. However, it remains unclear whether arginine-specific cysteine proteinase (Rgp) and lysine-specific proteinase (Kgp) play different roles in the detachment of A. actinomycetemcomitans biofilm. The aim of this study was to investigate possible disruptive roles of Kgp and Rgp in the aggregation and attachment of A. actinomycetemcomitans. While P. gingivalis ATCC33277 culture supernatant has an ability to decrease autoaggregation and coaggregation of A. actinomycetemcomitans cells, neither the boiled culture supernatant of ATCC33277 nor the culture supernatant of KDP136 showed this ability. The addition of KYT-1 and KYT-36, specific inhibitors of Rgp and Kgp, respectively, showed no influence on the ability of P. gingivalis culture supernatant. The result of gelatin zymography suggested that other proteases processed by gingipains mediated the decrease of A. actinomycetemcomitans aggregations. We also examined the biofilm-destructive effect of gingipains by assessing the detachment of A. actinomycetemcomitans from polystyrene surfaces. Scanning electron microscope analysis indicated that A. actinomycetemcomitans cells were detached by P. gingivalis Kgp. The quantity of A. actinomycetemcomitans in biofilm was decreased in co-culture with P. gingivalis. However, this was not found after the addition of KYT-36. These findings suggest that Kgp is a critical component for the detachment and decrease of A. actinomycetemcomitans biofilms.

Authors+Show Affiliations

Division of Oral Rehabilitation, Department of Periodontology, Faculty of Dental Science, Kyushu University, Fukuoka, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24661327

Citation

Haraguchi, A, et al. "Porphyromonas Gingivalis Gingipain Is Involved in the Detachment and Aggregation of Aggregatibacter Actinomycetemcomitans Biofilm." Molecular Oral Microbiology, vol. 29, no. 3, 2014, pp. 131-43.
Haraguchi A, Miura M, Fujise O, et al. Porphyromonas gingivalis gingipain is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm. Mol Oral Microbiol. 2014;29(3):131-43.
Haraguchi, A., Miura, M., Fujise, O., Hamachi, T., & Nishimura, F. (2014). Porphyromonas gingivalis gingipain is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm. Molecular Oral Microbiology, 29(3), 131-43. https://doi.org/10.1111/omi.12051
Haraguchi A, et al. Porphyromonas Gingivalis Gingipain Is Involved in the Detachment and Aggregation of Aggregatibacter Actinomycetemcomitans Biofilm. Mol Oral Microbiol. 2014;29(3):131-43. PubMed PMID: 24661327.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Porphyromonas gingivalis gingipain is involved in the detachment and aggregation of Aggregatibacter actinomycetemcomitans biofilm. AU - Haraguchi,A, AU - Miura,M, AU - Fujise,O, AU - Hamachi,T, AU - Nishimura,F, Y1 - 2014/04/10/ PY - 2014/03/18/accepted PY - 2014/3/26/entrez PY - 2014/3/26/pubmed PY - 2015/7/2/medline KW - Aggregatibacter actinomycetemcomitans KW - Porphyromonas gingivalis KW - biofilm KW - gingipains SP - 131 EP - 43 JF - Molecular oral microbiology JO - Mol Oral Microbiol VL - 29 IS - 3 N2 - Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans are major periodontal pathogens that cause several types of periodontal disease. Our previous study suggested that P. gingivalis gingipains secreted in the subgingival environment are related to the detachment of A.actinomycetemcomitans biofilms. However, it remains unclear whether arginine-specific cysteine proteinase (Rgp) and lysine-specific proteinase (Kgp) play different roles in the detachment of A. actinomycetemcomitans biofilm. The aim of this study was to investigate possible disruptive roles of Kgp and Rgp in the aggregation and attachment of A. actinomycetemcomitans. While P. gingivalis ATCC33277 culture supernatant has an ability to decrease autoaggregation and coaggregation of A. actinomycetemcomitans cells, neither the boiled culture supernatant of ATCC33277 nor the culture supernatant of KDP136 showed this ability. The addition of KYT-1 and KYT-36, specific inhibitors of Rgp and Kgp, respectively, showed no influence on the ability of P. gingivalis culture supernatant. The result of gelatin zymography suggested that other proteases processed by gingipains mediated the decrease of A. actinomycetemcomitans aggregations. We also examined the biofilm-destructive effect of gingipains by assessing the detachment of A. actinomycetemcomitans from polystyrene surfaces. Scanning electron microscope analysis indicated that A. actinomycetemcomitans cells were detached by P. gingivalis Kgp. The quantity of A. actinomycetemcomitans in biofilm was decreased in co-culture with P. gingivalis. However, this was not found after the addition of KYT-36. These findings suggest that Kgp is a critical component for the detachment and decrease of A. actinomycetemcomitans biofilms. SN - 2041-1014 UR - https://www.unboundmedicine.com/medline/citation/24661327/Porphyromonas_gingivalis_gingipain_is_involved_in_the_detachment_and_aggregation_of_Aggregatibacter_actinomycetemcomitans_biofilm_ L2 - https://doi.org/10.1111/omi.12051 DB - PRIME DP - Unbound Medicine ER -