Tags

Type your tag names separated by a space and hit enter

Detectability of new psychoactive substances, 'legal highs', in CEDIA, EMIT, and KIMS immunochemical screening assays for drugs of abuse.
Drug Test Anal 2014; 6(5):492-9DT

Abstract

The increasing number of new psychoactive substances made available for recreational drug use has created a challenge for clinical toxicology and drug testing laboratories. As a consequence, the routine immunoassay drug testing may become less effective due to an increased occurrence of false negative and false positive screening results. This work aimed to extend the knowledge about analytical cross-reactivity of new substances in selected CEDIA, EMIT, and KIMS immunoassays for drugs-of-abuse screening. Urine standards were prepared by spiking blank urine with 45 new substances. Authentic urine samples from intoxication cases identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were also studied. Several new psychoactive substances were demonstrated to display cross-reactivity in the immunoassays. CEDIA Amphetamine/Ecstasy and EMIT d.a.u. Amphetamine Class tests showed the highest reactivity towards the new drugs, which was expected since many have amphetamine-like structure and activity. In the samples from authentic cases, five new substances displayed 100% detection rate in the CEDIA Amphetamine/Ecstasy test. In conclusion, cross-reactivity data in routine urine drug screening immunoassays for a number of new psychoactive substances not studied before were reported. In both spiked and authentic urine samples, some new substances showed significant cross-reactivity and are thus detectable in the routine screening methods.

Authors+Show Affiliations

Department of Laboratory Medicine, section of Clinical Pharmacology, Karolinska University Hospital, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24665024

Citation

Beck, Olof, et al. "Detectability of New Psychoactive Substances, 'legal Highs', in CEDIA, EMIT, and KIMS Immunochemical Screening Assays for Drugs of Abuse." Drug Testing and Analysis, vol. 6, no. 5, 2014, pp. 492-9.
Beck O, Rausberg L, Al-Saffar Y, et al. Detectability of new psychoactive substances, 'legal highs', in CEDIA, EMIT, and KIMS immunochemical screening assays for drugs of abuse. Drug Test Anal. 2014;6(5):492-9.
Beck, O., Rausberg, L., Al-Saffar, Y., Villen, T., Karlsson, L., Hansson, T., & Helander, A. (2014). Detectability of new psychoactive substances, 'legal highs', in CEDIA, EMIT, and KIMS immunochemical screening assays for drugs of abuse. Drug Testing and Analysis, 6(5), pp. 492-9. doi:10.1002/dta.1641.
Beck O, et al. Detectability of New Psychoactive Substances, 'legal Highs', in CEDIA, EMIT, and KIMS Immunochemical Screening Assays for Drugs of Abuse. Drug Test Anal. 2014;6(5):492-9. PubMed PMID: 24665024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detectability of new psychoactive substances, 'legal highs', in CEDIA, EMIT, and KIMS immunochemical screening assays for drugs of abuse. AU - Beck,Olof, AU - Rausberg,Linnea, AU - Al-Saffar,Yasir, AU - Villen,Tomas, AU - Karlsson,Lennart, AU - Hansson,Therese, AU - Helander,Anders, Y1 - 2014/03/24/ PY - 2013/11/16/received PY - 2014/02/02/revised PY - 2014/02/15/accepted PY - 2014/3/26/entrez PY - 2014/3/26/pubmed PY - 2015/2/24/medline KW - CEDIA KW - EMIT KW - KIMS KW - drug screening KW - drug testing KW - immunoassay KW - new psychoactive substances KW - urine SP - 492 EP - 9 JF - Drug testing and analysis JO - Drug Test Anal VL - 6 IS - 5 N2 - The increasing number of new psychoactive substances made available for recreational drug use has created a challenge for clinical toxicology and drug testing laboratories. As a consequence, the routine immunoassay drug testing may become less effective due to an increased occurrence of false negative and false positive screening results. This work aimed to extend the knowledge about analytical cross-reactivity of new substances in selected CEDIA, EMIT, and KIMS immunoassays for drugs-of-abuse screening. Urine standards were prepared by spiking blank urine with 45 new substances. Authentic urine samples from intoxication cases identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were also studied. Several new psychoactive substances were demonstrated to display cross-reactivity in the immunoassays. CEDIA Amphetamine/Ecstasy and EMIT d.a.u. Amphetamine Class tests showed the highest reactivity towards the new drugs, which was expected since many have amphetamine-like structure and activity. In the samples from authentic cases, five new substances displayed 100% detection rate in the CEDIA Amphetamine/Ecstasy test. In conclusion, cross-reactivity data in routine urine drug screening immunoassays for a number of new psychoactive substances not studied before were reported. In both spiked and authentic urine samples, some new substances showed significant cross-reactivity and are thus detectable in the routine screening methods. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/24665024/Detectability_of_new_psychoactive_substances_'legal_highs'_in_CEDIA_EMIT_and_KIMS_immunochemical_screening_assays_for_drugs_of_abuse_ L2 - https://doi.org/10.1002/dta.1641 DB - PRIME DP - Unbound Medicine ER -