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Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250.
Electrophoresis. 1988 Jun; 9(6):255-62.E

Abstract

An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations of ammonium sulfate to the staining solution previously described. The method combines the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining. The method has been applied to staining of polyacrylamide gels after sodium dodecyl sulfate-electrophoresis and isoelectric focusing in carrier ampholyte-generated pH gradients.

Authors+Show Affiliations

Max-Planck-Institut für experimentelle Medizin, Göttingen, Federal Republic of Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2466658

Citation

Neuhoff, V, et al. "Improved Staining of Proteins in Polyacrylamide Gels Including Isoelectric Focusing Gels With Clear Background at Nanogram Sensitivity Using Coomassie Brilliant Blue G-250 and R-250." Electrophoresis, vol. 9, no. 6, 1988, pp. 255-62.
Neuhoff V, Arold N, Taube D, et al. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis. 1988;9(6):255-62.
Neuhoff, V., Arold, N., Taube, D., & Ehrhardt, W. (1988). Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis, 9(6), 255-62.
Neuhoff V, et al. Improved Staining of Proteins in Polyacrylamide Gels Including Isoelectric Focusing Gels With Clear Background at Nanogram Sensitivity Using Coomassie Brilliant Blue G-250 and R-250. Electrophoresis. 1988;9(6):255-62. PubMed PMID: 2466658.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G-250 and R-250. AU - Neuhoff,V, AU - Arold,N, AU - Taube,D, AU - Ehrhardt,W, PY - 1988/6/1/pubmed PY - 1988/6/1/medline PY - 1988/6/1/entrez SP - 255 EP - 62 JF - Electrophoresis JO - Electrophoresis VL - 9 IS - 6 N2 - An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations of ammonium sulfate to the staining solution previously described. The method combines the advantage of much shorter staining time with high sensitivity, a clear background not requiring destaining, stepwise staining, and stable fixation after staining. The method has been applied to staining of polyacrylamide gels after sodium dodecyl sulfate-electrophoresis and isoelectric focusing in carrier ampholyte-generated pH gradients. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/2466658/Improved_staining_of_proteins_in_polyacrylamide_gels_including_isoelectric_focusing_gels_with_clear_background_at_nanogram_sensitivity_using_Coomassie_Brilliant_Blue_G_250_and_R_250_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0173-0835&date=1988&volume=9&issue=6&spage=255 DB - PRIME DP - Unbound Medicine ER -