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Analysis of volatile compounds from Siraitia grosvenorii by headspace solid-phase microextraction and gas chromatography-quadrupole time-of-flight mass spectrometry.
J Chromatogr Sci. 2015 Jan; 53(1):1-7.JC

Abstract

Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF MS) has been used in the present study to isolate and identify volatile components from Siraitia grosvenorii, a herbaceous perennial vine used as a natural sweetener and medicine. Compared with the static HS extraction and the different SPME fiber coatings, HS-SPME with divinylbenzene/carboxen/polydimethylsiloxane fiber was preferred to extract the target analytes including aldehyde, alcohol, acid, ester and other compounds. In the identification, a multi-dimensional qualitative analysis approach containing library searching, retention index comparison, accurate mass measurement, the second-stage mass spectrum (MS(2) spectrum) and area normalization was utilized. Finally, a total of 46 compounds were identified from S. grosvenorii. Among them, n-hexadecanoic acid, 5-hydroxymethylfurfural, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, (Z, Z)-9,12-octadecadienoic acid and (E, E)-2,4-nonadienal were the main volatile components. The results showed that HS-SPME coupled with GC-QTOF MS is efficient for the extraction of polar volatiles from S. grosvenorii and accurate for the identification of volatile compounds in the complicated matrix.

Authors+Show Affiliations

Jinling Pharmaceutical Co., Ltd, 238 Zhongyang Road, Nanjing 210009, People's Republic of China School of Life Science, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, People's Republic of China Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, People's Republic of China.Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, People's Republic of China.School of Life Science, Anhui Agricultural University, 130 West Changjiang Road, Hefei 230036, People's Republic of China ylguo@sioc.ac.cn.Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, 345 Lingling Road, Shanghai 200032, People's Republic of China ylguo@sioc.ac.cn.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24668041

Citation

Xia, Yi, et al. "Analysis of Volatile Compounds From Siraitia Grosvenorii By Headspace Solid-phase Microextraction and Gas Chromatography-quadrupole Time-of-flight Mass Spectrometry." Journal of Chromatographic Science, vol. 53, no. 1, 2015, pp. 1-7.
Xia Y, Zhang F, Wang W, et al. Analysis of volatile compounds from Siraitia grosvenorii by headspace solid-phase microextraction and gas chromatography-quadrupole time-of-flight mass spectrometry. J Chromatogr Sci. 2015;53(1):1-7.
Xia, Y., Zhang, F., Wang, W., & Guo, Y. (2015). Analysis of volatile compounds from Siraitia grosvenorii by headspace solid-phase microextraction and gas chromatography-quadrupole time-of-flight mass spectrometry. Journal of Chromatographic Science, 53(1), 1-7. https://doi.org/10.1093/chromsci/bmu012
Xia Y, et al. Analysis of Volatile Compounds From Siraitia Grosvenorii By Headspace Solid-phase Microextraction and Gas Chromatography-quadrupole Time-of-flight Mass Spectrometry. J Chromatogr Sci. 2015;53(1):1-7. PubMed PMID: 24668041.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of volatile compounds from Siraitia grosvenorii by headspace solid-phase microextraction and gas chromatography-quadrupole time-of-flight mass spectrometry. AU - Xia,Yi, AU - Zhang,Fang, AU - Wang,Weiyun, AU - Guo,Yinlong, Y1 - 2014/03/24/ PY - 2014/3/27/entrez PY - 2014/3/29/pubmed PY - 2015/8/4/medline SP - 1 EP - 7 JF - Journal of chromatographic science JO - J Chromatogr Sci VL - 53 IS - 1 N2 - Headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-quadrupole time-of-flight mass spectrometry (GC-QTOF MS) has been used in the present study to isolate and identify volatile components from Siraitia grosvenorii, a herbaceous perennial vine used as a natural sweetener and medicine. Compared with the static HS extraction and the different SPME fiber coatings, HS-SPME with divinylbenzene/carboxen/polydimethylsiloxane fiber was preferred to extract the target analytes including aldehyde, alcohol, acid, ester and other compounds. In the identification, a multi-dimensional qualitative analysis approach containing library searching, retention index comparison, accurate mass measurement, the second-stage mass spectrum (MS(2) spectrum) and area normalization was utilized. Finally, a total of 46 compounds were identified from S. grosvenorii. Among them, n-hexadecanoic acid, 5-hydroxymethylfurfural, 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one, (Z, Z)-9,12-octadecadienoic acid and (E, E)-2,4-nonadienal were the main volatile components. The results showed that HS-SPME coupled with GC-QTOF MS is efficient for the extraction of polar volatiles from S. grosvenorii and accurate for the identification of volatile compounds in the complicated matrix. SN - 1945-239X UR - https://www.unboundmedicine.com/medline/citation/24668041/Analysis_of_volatile_compounds_from_Siraitia_grosvenorii_by_headspace_solid_phase_microextraction_and_gas_chromatography_quadrupole_time_of_flight_mass_spectrometry_ L2 - https://academic.oup.com/chromsci/article-lookup/doi/10.1093/chromsci/bmu012 DB - PRIME DP - Unbound Medicine ER -