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Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress.

Abstract

BACKGROUND

cis-Diammineplatinum (II) dichloride (cisplatin) is the important anti-cancer agent useful in treatment of various cancers. Unfortunately, it can produce unwanted side effects in various tissues, including the liver. The present study investigated the possible protective role of curcumin and α-tocopherol against oxidative stress-induced hepatotoxicity in rats upon cisplatin treatment.

METHODS

Male Wistar rats were divided into five groups (n = 5). Saline and Cis groups, rats were intraperitoneal (i.p.) injected with normal saline and cisplatin [20 mg/kg body weight (b.w.)], respectively. Cis + α-tocopherol group, Cis + Cur group and Cis + α-tocopherol + Cur group, rats were pre-treated with a single dose of α-tocopherol (250 mg/kg b.w.), curcumin (200 mg/kg b.w.) and combined α-tocopherol with curcumin, respectively, for 24 h prior the administration of cisplatin. After 72 h of first injection, specimens were collected. Liver enzyme, lipid peroxidation biomarker, liver histopathology and gene expression of liver nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were investigated.

RESULTS

Cisplatin revealed a significant increase of hepatic malondialdehyde (MDA) levels and a significant reduction of hepatic superoxide dismutase (SOD) and catalase activities compared to the saline group. It elicited a marked increase of the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and demonstrated the liver pathologies including liver congestion, disorganization of hepatic cords and ground glass appearance of hepatocytes. It also demonstrated a significant increase of NADPH oxidase gene expression compared to saline group. Pre-treatment with combined curcumin and α-tocopherol improved the liver enzymes, lipid peroxidation biomarker, liver histopathology and gene expression of liver NADPH oxidase in cisplatin-treated rats.

CONCLUSIONS

The findings indicate that pre-treatment with combined curcumin and α-tocopherol can protect cisplatin-induced hepatotoxicity including the biochemical, histological and molecular aspects. The down-regulations of NADPH oxidase gene expression may be involved in abrogating oxidative stress via reduction of reactive oxygen species (ROS) production.

Authors+Show Affiliations

School of Medicine, Walailak University, Nakhon Si Thammarat 80161, Thailand. spalipoch@hotmail.com.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24674233

Citation

Palipoch, Sarawoot, et al. "Hepatoprotective Effect of Curcumin and Alpha-tocopherol Against Cisplatin-induced Oxidative Stress." BMC Complementary and Alternative Medicine, vol. 14, 2014, p. 111.
Palipoch S, Punsawad C, Koomhin P, et al. Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress. BMC Complement Altern Med. 2014;14:111.
Palipoch, S., Punsawad, C., Koomhin, P., & Suwannalert, P. (2014). Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress. BMC Complementary and Alternative Medicine, 14, p. 111. doi:10.1186/1472-6882-14-111.
Palipoch S, et al. Hepatoprotective Effect of Curcumin and Alpha-tocopherol Against Cisplatin-induced Oxidative Stress. BMC Complement Altern Med. 2014 Mar 28;14:111. PubMed PMID: 24674233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatoprotective effect of curcumin and alpha-tocopherol against cisplatin-induced oxidative stress. AU - Palipoch,Sarawoot, AU - Punsawad,Chuchard, AU - Koomhin,Phanit, AU - Suwannalert,Prasit, Y1 - 2014/03/28/ PY - 2013/11/12/received PY - 2014/03/24/accepted PY - 2014/3/29/entrez PY - 2014/3/29/pubmed PY - 2015/5/26/medline SP - 111 EP - 111 JF - BMC complementary and alternative medicine JO - BMC Complement Altern Med VL - 14 N2 - BACKGROUND: cis-Diammineplatinum (II) dichloride (cisplatin) is the important anti-cancer agent useful in treatment of various cancers. Unfortunately, it can produce unwanted side effects in various tissues, including the liver. The present study investigated the possible protective role of curcumin and α-tocopherol against oxidative stress-induced hepatotoxicity in rats upon cisplatin treatment. METHODS: Male Wistar rats were divided into five groups (n = 5). Saline and Cis groups, rats were intraperitoneal (i.p.) injected with normal saline and cisplatin [20 mg/kg body weight (b.w.)], respectively. Cis + α-tocopherol group, Cis + Cur group and Cis + α-tocopherol + Cur group, rats were pre-treated with a single dose of α-tocopherol (250 mg/kg b.w.), curcumin (200 mg/kg b.w.) and combined α-tocopherol with curcumin, respectively, for 24 h prior the administration of cisplatin. After 72 h of first injection, specimens were collected. Liver enzyme, lipid peroxidation biomarker, liver histopathology and gene expression of liver nicotinamide adenine dinucleotide phosphate (NADPH) oxidase were investigated. RESULTS: Cisplatin revealed a significant increase of hepatic malondialdehyde (MDA) levels and a significant reduction of hepatic superoxide dismutase (SOD) and catalase activities compared to the saline group. It elicited a marked increase of the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and demonstrated the liver pathologies including liver congestion, disorganization of hepatic cords and ground glass appearance of hepatocytes. It also demonstrated a significant increase of NADPH oxidase gene expression compared to saline group. Pre-treatment with combined curcumin and α-tocopherol improved the liver enzymes, lipid peroxidation biomarker, liver histopathology and gene expression of liver NADPH oxidase in cisplatin-treated rats. CONCLUSIONS: The findings indicate that pre-treatment with combined curcumin and α-tocopherol can protect cisplatin-induced hepatotoxicity including the biochemical, histological and molecular aspects. The down-regulations of NADPH oxidase gene expression may be involved in abrogating oxidative stress via reduction of reactive oxygen species (ROS) production. SN - 1472-6882 UR - https://www.unboundmedicine.com/medline/citation/24674233/Hepatoprotective_effect_of_curcumin_and_alpha_tocopherol_against_cisplatin_induced_oxidative_stress_ L2 - https://bmccomplementalternmed.biomedcentral.com/articles/10.1186/1472-6882-14-111 DB - PRIME DP - Unbound Medicine ER -