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Hepatic β-oxidation and regulation of carnitine palmitoyltransferase (CPT) I in blunt snout bream Megalobrama amblycephala fed a high fat diet.
PLoS One. 2014; 9(3):e93135.Plos

Abstract

High-fat diets may promote growth, partly through their protein-sparing effects. However, high-fat diets often lead to excessive fat deposition, which may have a negative impact on fish such as poor growth and suppressive immune. Therefore, this study investigated the effects of a fat-rich diet on the mechanisms of fat deposition in the liver. Three-hundred blunt snout bream (Megalobrama amblycephala) juveniles (initial mass 18.00 ± 0.05 g) were fed with one of two diets (5% or 15% fat) for 8 weeks. β-Oxidation capacity and regulation of rate-limiting enzymes were assessed. Large fat droplets were present in hepatocytes of fish fed the high-fat diet. This observation is thought to be largely owing to the reduced capacity for mitochondrial and peroxisomal β-oxidation in the livers of fish fed the high-fat diet, as well as the decreased activities of carnitine palmitoyltransferase (CPT) I and acyl-CoA oxidase (ACO), which are enzymes involved in fatty-acid metabolism. Study of CPT I kinetics showed that CPT I had a low affinity for its substrates and a low catalytic efficiency in fish fed the high-fat diet. Expression of both CPT I and ACO was significantly down-regulated in fish fed the high-fat diet. Moreover, the fatty-acid composition of the mitochondrial membrane varied between the two groups. In conclusion, the attenuated β-oxidation capacity observed in fish fed a high-fat diet is proposed to be owing to decreased activity and/or catalytic efficiency of the rate-limiting enzymes CPT I and ACO, via both genetic and non-genetic mechanisms.

Authors+Show Affiliations

College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu province, People's Republic of China.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu province, People's Republic of China.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu province, People's Republic of China.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu province, People's Republic of China.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu province, People's Republic of China.College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, Jiangsu province, People's Republic of China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24676148

Citation

Lu, Kang-Le, et al. "Hepatic Β-oxidation and Regulation of Carnitine Palmitoyltransferase (CPT) I in Blunt Snout Bream Megalobrama Amblycephala Fed a High Fat Diet." PloS One, vol. 9, no. 3, 2014, pp. e93135.
Lu KL, Xu WN, Wang LN, et al. Hepatic β-oxidation and regulation of carnitine palmitoyltransferase (CPT) I in blunt snout bream Megalobrama amblycephala fed a high fat diet. PLoS ONE. 2014;9(3):e93135.
Lu, K. L., Xu, W. N., Wang, L. N., Zhang, D. D., Zhang, C. N., & Liu, W. B. (2014). Hepatic β-oxidation and regulation of carnitine palmitoyltransferase (CPT) I in blunt snout bream Megalobrama amblycephala fed a high fat diet. PloS One, 9(3), e93135. https://doi.org/10.1371/journal.pone.0093135
Lu KL, et al. Hepatic Β-oxidation and Regulation of Carnitine Palmitoyltransferase (CPT) I in Blunt Snout Bream Megalobrama Amblycephala Fed a High Fat Diet. PLoS ONE. 2014;9(3):e93135. PubMed PMID: 24676148.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hepatic β-oxidation and regulation of carnitine palmitoyltransferase (CPT) I in blunt snout bream Megalobrama amblycephala fed a high fat diet. AU - Lu,Kang-Le, AU - Xu,Wei-Na, AU - Wang,Li-Na, AU - Zhang,Ding-Dong, AU - Zhang,Chun-Nuan, AU - Liu,Wen-Bin, Y1 - 2014/03/27/ PY - 2013/11/27/received PY - 2014/03/01/accepted PY - 2014/3/29/entrez PY - 2014/3/29/pubmed PY - 2015/12/19/medline SP - e93135 EP - e93135 JF - PloS one JO - PLoS ONE VL - 9 IS - 3 N2 - High-fat diets may promote growth, partly through their protein-sparing effects. However, high-fat diets often lead to excessive fat deposition, which may have a negative impact on fish such as poor growth and suppressive immune. Therefore, this study investigated the effects of a fat-rich diet on the mechanisms of fat deposition in the liver. Three-hundred blunt snout bream (Megalobrama amblycephala) juveniles (initial mass 18.00 ± 0.05 g) were fed with one of two diets (5% or 15% fat) for 8 weeks. β-Oxidation capacity and regulation of rate-limiting enzymes were assessed. Large fat droplets were present in hepatocytes of fish fed the high-fat diet. This observation is thought to be largely owing to the reduced capacity for mitochondrial and peroxisomal β-oxidation in the livers of fish fed the high-fat diet, as well as the decreased activities of carnitine palmitoyltransferase (CPT) I and acyl-CoA oxidase (ACO), which are enzymes involved in fatty-acid metabolism. Study of CPT I kinetics showed that CPT I had a low affinity for its substrates and a low catalytic efficiency in fish fed the high-fat diet. Expression of both CPT I and ACO was significantly down-regulated in fish fed the high-fat diet. Moreover, the fatty-acid composition of the mitochondrial membrane varied between the two groups. In conclusion, the attenuated β-oxidation capacity observed in fish fed a high-fat diet is proposed to be owing to decreased activity and/or catalytic efficiency of the rate-limiting enzymes CPT I and ACO, via both genetic and non-genetic mechanisms. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24676148/Hepatic_β_oxidation_and_regulation_of_carnitine_palmitoyltransferase__CPT__I_in_blunt_snout_bream_Megalobrama_amblycephala_fed_a_high_fat_diet_ L2 - http://dx.plos.org/10.1371/journal.pone.0093135 DB - PRIME DP - Unbound Medicine ER -