Tags

Type your tag names separated by a space and hit enter

Next-generation snake venomics: protein-locus resolution through venom proteome decomplexation.
Expert Rev Proteomics. 2014 Jun; 11(3):315-29.ER

Abstract

Venom research has been continuously enhanced by technological advances. High-throughput technologies are changing the classical paradigm of hypothesis-driven research to technology-driven approaches. However, the thesis advocated in this paper is that full proteome coverage at locus-specific resolution requires integrating the best of both worlds into a protocol that includes decomplexation of the venom proteome prior to liquid chromatography-tandem mass spectrometry matching against a species-specific transcriptome. This approach offers the possibility of proof-checking the species-specific contig database using proteomics data. Immunoaffinity chromatography constitutes the basis of an antivenomics workflow designed to quantify the extent of cross-reactivity of antivenoms against homologous and heterologous venom toxins. In the author's view, snake venomics and antivenomics form part of a biology-driven conceptual framework to unveil the genesis and natural history of venoms, and their within- and between-species toxicological and immunological divergences and similarities. Understanding evolutionary trends across venoms represents the Rosetta Stone for generating broad-ranging polyspecific antivenoms.

Authors+Show Affiliations

Instituto de Biomedicina de Valencia, Consejo Superior de Investigaciones Científicas, Jaime Roig 11, 46010 Valencia, Spain +34 963 391 778 +34 963 690 800 jcalvete@ibv.csic.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

24678852

Citation

Calvete, Juan J.. "Next-generation Snake Venomics: Protein-locus Resolution Through Venom Proteome Decomplexation." Expert Review of Proteomics, vol. 11, no. 3, 2014, pp. 315-29.
Calvete JJ. Next-generation snake venomics: protein-locus resolution through venom proteome decomplexation. Expert Rev Proteomics. 2014;11(3):315-29.
Calvete, J. J. (2014). Next-generation snake venomics: protein-locus resolution through venom proteome decomplexation. Expert Review of Proteomics, 11(3), 315-29. https://doi.org/10.1586/14789450.2014.900447
Calvete JJ. Next-generation Snake Venomics: Protein-locus Resolution Through Venom Proteome Decomplexation. Expert Rev Proteomics. 2014;11(3):315-29. PubMed PMID: 24678852.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Next-generation snake venomics: protein-locus resolution through venom proteome decomplexation. A1 - Calvete,Juan J, Y1 - 2014/03/29/ PY - 2014/4/1/entrez PY - 2014/4/1/pubmed PY - 2015/4/25/medline KW - affinity chromatography KW - antivenom KW - antivenomics KW - mass spectrometry KW - snake venom KW - snakebite envenoming KW - venomics SP - 315 EP - 29 JF - Expert review of proteomics JO - Expert Rev Proteomics VL - 11 IS - 3 N2 - Venom research has been continuously enhanced by technological advances. High-throughput technologies are changing the classical paradigm of hypothesis-driven research to technology-driven approaches. However, the thesis advocated in this paper is that full proteome coverage at locus-specific resolution requires integrating the best of both worlds into a protocol that includes decomplexation of the venom proteome prior to liquid chromatography-tandem mass spectrometry matching against a species-specific transcriptome. This approach offers the possibility of proof-checking the species-specific contig database using proteomics data. Immunoaffinity chromatography constitutes the basis of an antivenomics workflow designed to quantify the extent of cross-reactivity of antivenoms against homologous and heterologous venom toxins. In the author's view, snake venomics and antivenomics form part of a biology-driven conceptual framework to unveil the genesis and natural history of venoms, and their within- and between-species toxicological and immunological divergences and similarities. Understanding evolutionary trends across venoms represents the Rosetta Stone for generating broad-ranging polyspecific antivenoms. SN - 1744-8387 UR - https://www.unboundmedicine.com/medline/citation/24678852/Next_generation_snake_venomics:_protein_locus_resolution_through_venom_proteome_decomplexation_ L2 - https://www.tandfonline.com/doi/full/10.1586/14789450.2014.900447 DB - PRIME DP - Unbound Medicine ER -