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Inhibition of acid-sensing ion channels by chlorogenic acid in rat dorsal root ganglion neurons.
Neurosci Lett. 2014 May 01; 567:35-9.NL

Abstract

Chlorogenic acid (CGA) is one of the most abundant polyphenol compounds in the human diet. Recently, it is demonstrated to have potent antinociceptive effect. However, little is understood about the mechanism underlying CGA analgesia. Here, we have found that CGA can exert an inhibitory effect on the functional activity of native acid-sensing ion channels (ASICs) in rat dorsal root ganglion (DRG) neurons. First, CGA decreased the peak amplitude of proton-gated currents mediated by ASICs in a concentration-dependent manner. Second, CGA shifted the proton concentration-response curve downward, with a decrease of 41.76 ± 8.65% in the maximum current response to protons but with no significant change in the pH0.5 value. Third, CGA altered acidosis-evoked membrane excitability of rat DRG neurons and caused a significant decrease in the amplitude of the depolarization and the number of action potentials induced by acid stimuli. Finally, peripheral administered CGA attenuated nociceptive response to intraplantar injection of acetic acid in rats. ASICs are distributed in peripheral sensory neurons and participate in nociception. Our findings CGA inhibition of native ASICs indicated that CGA may exert analgesic action by modulating ASICs in the primary afferent neurons, which revealed a novel cellular and molecular mechanism underlying CGA analgesia.

Authors+Show Affiliations

Department of Pharmacology, Hubei University of Science and Technology, 88 Xianning Road, Xianning, 437100, Hubei, PR China.Department of Pharmacology, Hubei University of Science and Technology, 88 Xianning Road, Xianning, 437100, Hubei, PR China.Department of Pharmacology, Hubei University of Science and Technology, 88 Xianning Road, Xianning, 437100, Hubei, PR China.Department of Pharmacology, Hubei University of Science and Technology, 88 Xianning Road, Xianning, 437100, Hubei, PR China.Department of Pharmacology, Hubei University of Science and Technology, 88 Xianning Road, Xianning, 437100, Hubei, PR China. Electronic address: wangping_hu@163.com.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24680850

Citation

Qu, Zu-Wei, et al. "Inhibition of Acid-sensing Ion Channels By Chlorogenic Acid in Rat Dorsal Root Ganglion Neurons." Neuroscience Letters, vol. 567, 2014, pp. 35-9.
Qu ZW, Liu TT, Qiu CY, et al. Inhibition of acid-sensing ion channels by chlorogenic acid in rat dorsal root ganglion neurons. Neurosci Lett. 2014;567:35-9.
Qu, Z. W., Liu, T. T., Qiu, C. Y., Li, J. D., & Hu, W. P. (2014). Inhibition of acid-sensing ion channels by chlorogenic acid in rat dorsal root ganglion neurons. Neuroscience Letters, 567, 35-9. https://doi.org/10.1016/j.neulet.2014.03.027
Qu ZW, et al. Inhibition of Acid-sensing Ion Channels By Chlorogenic Acid in Rat Dorsal Root Ganglion Neurons. Neurosci Lett. 2014 May 1;567:35-9. PubMed PMID: 24680850.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of acid-sensing ion channels by chlorogenic acid in rat dorsal root ganglion neurons. AU - Qu,Zu-Wei, AU - Liu,Ting-Ting, AU - Qiu,Chun-Yu, AU - Li,Jia-Da, AU - Hu,Wang-Ping, Y1 - 2014/03/26/ PY - 2014/01/09/received PY - 2014/03/15/revised PY - 2014/03/17/accepted PY - 2014/4/1/entrez PY - 2014/4/1/pubmed PY - 2014/11/9/medline KW - Acid-sensing ion channel KW - Chlorogenic acid KW - Dorsal root ganglion neuron KW - Pain KW - Proton-gated current SP - 35 EP - 9 JF - Neuroscience letters JO - Neurosci Lett VL - 567 N2 - Chlorogenic acid (CGA) is one of the most abundant polyphenol compounds in the human diet. Recently, it is demonstrated to have potent antinociceptive effect. However, little is understood about the mechanism underlying CGA analgesia. Here, we have found that CGA can exert an inhibitory effect on the functional activity of native acid-sensing ion channels (ASICs) in rat dorsal root ganglion (DRG) neurons. First, CGA decreased the peak amplitude of proton-gated currents mediated by ASICs in a concentration-dependent manner. Second, CGA shifted the proton concentration-response curve downward, with a decrease of 41.76 ± 8.65% in the maximum current response to protons but with no significant change in the pH0.5 value. Third, CGA altered acidosis-evoked membrane excitability of rat DRG neurons and caused a significant decrease in the amplitude of the depolarization and the number of action potentials induced by acid stimuli. Finally, peripheral administered CGA attenuated nociceptive response to intraplantar injection of acetic acid in rats. ASICs are distributed in peripheral sensory neurons and participate in nociception. Our findings CGA inhibition of native ASICs indicated that CGA may exert analgesic action by modulating ASICs in the primary afferent neurons, which revealed a novel cellular and molecular mechanism underlying CGA analgesia. SN - 1872-7972 UR - https://www.unboundmedicine.com/medline/citation/24680850/Inhibition_of_acid_sensing_ion_channels_by_chlorogenic_acid_in_rat_dorsal_root_ganglion_neurons_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304-3940(14)00226-2 DB - PRIME DP - Unbound Medicine ER -