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The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity.
Gen Comp Endocrinol. 2014 Oct 01; 207:94-102.GC

Abstract

The native distribution of Mozambique tilapia, Oreochromis mossambicus, is characterized by estuarine areas subject to salinity variations between fresh water (FW) and seawater (SW) with tidal frequency. Osmoregulation in the face of changing environmental salinity is largely mediated through the neuroendocrine system and involves the activation of ion uptake and extrusion mechanisms in osmoregulatory tissues. We compared plasma osmolality, plasma prolactin (PRL), pituitary PRL mRNA, and mRNA of branchial ion pumps, transporters, channels, and PRL receptors in tilapia reared in FW, SW, brackish water (BW) and in tidally-changing salinity, which varied between FW (TF) and SW (TS) every 6h. Plasma PRL was higher in FW tilapia than in SW, BW, TF, and TS tilapia. Unlike tilapia reared in FW or SW, fish in salinities that varied tidally showed no correlation between plasma osmolality and PRL. In FW fish, gene expression of PRL receptor 1 (PRLR1), Na(+)/Cl(-) cotransporter (NCC), aquaporin 3 (AQP3) and two isoforms of Na(+)/K(+)-ATPase (NKA α1a and NKA α1b) was higher than that of SW, BW or tidally-changing salinity fish. Gene expression of the Na(+)/K(+)/2Cl(-) cotransporter (NKCC1a), and the cystic fibrosis transmembrane conductance regulator (CFTR) were higher in fish in SW, BW or a tidally-changing salinity than in FW fish. Immunocytochemistry revealed that ionocytes of fish in tidally-changing salinities resemble ionocytes of SW fish. This study indicated that tilapia reared in a tidally-changing salinity can compensate for large changes in external osmolality while maintaining osmoregulatory parameters within a narrow range closer to that observed in SW-acclimated fish.

Authors+Show Affiliations

Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA; Department of Molecular Biosciences and Bioengineering, University of Hawaii at Manoa, Honolulu, HI 96822, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA; University of Hawaii Sea Grant College Program, University of Hawaii at Manoa, Honolulu, HI 96822, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA.Hawaii Institute of Marine Biology, University of Hawaii, Kaneohe, HI 96744, USA. Electronic address: seale@hawaii.edu.

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

24681189

Citation

Moorman, Benjamin P., et al. "The Osmoregulatory Effects of Rearing Mozambique Tilapia in a Tidally Changing Salinity." General and Comparative Endocrinology, vol. 207, 2014, pp. 94-102.
Moorman BP, Inokuchi M, Yamaguchi Y, et al. The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity. Gen Comp Endocrinol. 2014;207:94-102.
Moorman, B. P., Inokuchi, M., Yamaguchi, Y., Lerner, D. T., Grau, E. G., & Seale, A. P. (2014). The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity. General and Comparative Endocrinology, 207, 94-102. https://doi.org/10.1016/j.ygcen.2014.03.013
Moorman BP, et al. The Osmoregulatory Effects of Rearing Mozambique Tilapia in a Tidally Changing Salinity. Gen Comp Endocrinol. 2014 Oct 1;207:94-102. PubMed PMID: 24681189.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The osmoregulatory effects of rearing Mozambique tilapia in a tidally changing salinity. AU - Moorman,Benjamin P, AU - Inokuchi,Mayu, AU - Yamaguchi,Yoko, AU - Lerner,Darren T, AU - Grau,E Gordon, AU - Seale,Andre P, Y1 - 2014/03/27/ PY - 2014/01/03/received PY - 2014/02/20/revised PY - 2014/03/10/accepted PY - 2014/4/1/entrez PY - 2014/4/1/pubmed PY - 2015/5/1/medline KW - Ion transporter KW - Osmoregulation KW - Prolactin KW - Rearing salinity KW - Tidal cycle KW - Tilapia SP - 94 EP - 102 JF - General and comparative endocrinology JO - Gen Comp Endocrinol VL - 207 N2 - The native distribution of Mozambique tilapia, Oreochromis mossambicus, is characterized by estuarine areas subject to salinity variations between fresh water (FW) and seawater (SW) with tidal frequency. Osmoregulation in the face of changing environmental salinity is largely mediated through the neuroendocrine system and involves the activation of ion uptake and extrusion mechanisms in osmoregulatory tissues. We compared plasma osmolality, plasma prolactin (PRL), pituitary PRL mRNA, and mRNA of branchial ion pumps, transporters, channels, and PRL receptors in tilapia reared in FW, SW, brackish water (BW) and in tidally-changing salinity, which varied between FW (TF) and SW (TS) every 6h. Plasma PRL was higher in FW tilapia than in SW, BW, TF, and TS tilapia. Unlike tilapia reared in FW or SW, fish in salinities that varied tidally showed no correlation between plasma osmolality and PRL. In FW fish, gene expression of PRL receptor 1 (PRLR1), Na(+)/Cl(-) cotransporter (NCC), aquaporin 3 (AQP3) and two isoforms of Na(+)/K(+)-ATPase (NKA α1a and NKA α1b) was higher than that of SW, BW or tidally-changing salinity fish. Gene expression of the Na(+)/K(+)/2Cl(-) cotransporter (NKCC1a), and the cystic fibrosis transmembrane conductance regulator (CFTR) were higher in fish in SW, BW or a tidally-changing salinity than in FW fish. Immunocytochemistry revealed that ionocytes of fish in tidally-changing salinities resemble ionocytes of SW fish. This study indicated that tilapia reared in a tidally-changing salinity can compensate for large changes in external osmolality while maintaining osmoregulatory parameters within a narrow range closer to that observed in SW-acclimated fish. SN - 1095-6840 UR - https://www.unboundmedicine.com/medline/citation/24681189/The_osmoregulatory_effects_of_rearing_Mozambique_tilapia_in_a_tidally_changing_salinity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-6480(14)00087-2 DB - PRIME DP - Unbound Medicine ER -