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Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource.
PLoS One. 2014; 9(3):e93275.Plos

Abstract

Twenty-six orthologs of the rice blast resistance gene Pid3 from cultivated varieties and wild rice accessions distributed in different areas were cloned by allele mining. Sequence analysis showed that while each of the orthologous genes from indica varieties and most wild accessions encodes a complete NBS-LRR protein, each of the proteins encoded by those from japonica varieties and few wild rice accessions presents a premature termination. Eleven of the 26 orthologs were selected for blast resistance testing by transforming into the blast susceptible rice variety TP309, respectively. Inoculation of 23 M. oryzae strains collected from diverse regions of China to the respective transgenic plants revealed that 6 Pid3 orthologs showed susceptible to all the tested strains, while the other 5 orthologs showed differential resistance spectra in a gradually spectrum-widen order as Pid3-W3, Pid3-W4, Pid3-I3, Pid3-W5 and Pid3-I1. Amino acid sequences alignment of these orthologs indicated that the sequence diversities between the blast resistance orthologs were mostly located in the LRR domain such as the substitutions of Q694H,D856H,Q896R,D899E etc. However, the differences between the resistance orthologs and the susceptible ones were mostly located in the NBS domain. The present experiments provide an example of that the ortholog evaluation of plant R genes could be an efficient way to expand the rice blast resistance and some other plant disease resistance as well for breeding.

Authors+Show Affiliations

National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China; State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China; Rice Research Institute, Sichuan Agricultural University, Chengdu, China; National Key Laboratory of Hybrid Rice, Changsha, Hunan, China.Key Laboratory of Molecular Biology and Gene Engineering, College of Life Science, Nanchang University, Nanchang, China.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China.National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.Rice Research Institute, Sichuan Agricultural University, Chengdu, China.National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing, China.State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Science, Bejing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24681716

Citation

Xu, Xiao, et al. "Excavation of Pid3 Orthologs With Differential Resistance Spectra to Magnaporthe Oryzae in Rice Resource." PloS One, vol. 9, no. 3, 2014, pp. e93275.
Xu X, Lv Q, Shang J, et al. Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource. PLoS One. 2014;9(3):e93275.
Xu, X., Lv, Q., Shang, J., Pang, Z., Zhou, Z., Wang, J., Jiang, G., Tao, Y., Xu, Q., Li, X., Zhao, X., Li, S., Xu, J., & Zhu, L. (2014). Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource. PloS One, 9(3), e93275. https://doi.org/10.1371/journal.pone.0093275
Xu X, et al. Excavation of Pid3 Orthologs With Differential Resistance Spectra to Magnaporthe Oryzae in Rice Resource. PLoS One. 2014;9(3):e93275. PubMed PMID: 24681716.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Excavation of Pid3 orthologs with differential resistance spectra to Magnaporthe oryzae in rice resource. AU - Xu,Xiao, AU - Lv,Qiming, AU - Shang,Junjun, AU - Pang,Zhiqian, AU - Zhou,Zhuangzhi, AU - Wang,Jing, AU - Jiang,Guanghuai, AU - Tao,Yong, AU - Xu,Qian, AU - Li,Xiaobing, AU - Zhao,Xianfeng, AU - Li,Shigui, AU - Xu,Jichen, AU - Zhu,Lihuang, Y1 - 2014/03/28/ PY - 2013/12/23/received PY - 2014/03/02/accepted PY - 2014/4/1/entrez PY - 2014/4/1/pubmed PY - 2015/6/2/medline SP - e93275 EP - e93275 JF - PloS one JO - PLoS One VL - 9 IS - 3 N2 - Twenty-six orthologs of the rice blast resistance gene Pid3 from cultivated varieties and wild rice accessions distributed in different areas were cloned by allele mining. Sequence analysis showed that while each of the orthologous genes from indica varieties and most wild accessions encodes a complete NBS-LRR protein, each of the proteins encoded by those from japonica varieties and few wild rice accessions presents a premature termination. Eleven of the 26 orthologs were selected for blast resistance testing by transforming into the blast susceptible rice variety TP309, respectively. Inoculation of 23 M. oryzae strains collected from diverse regions of China to the respective transgenic plants revealed that 6 Pid3 orthologs showed susceptible to all the tested strains, while the other 5 orthologs showed differential resistance spectra in a gradually spectrum-widen order as Pid3-W3, Pid3-W4, Pid3-I3, Pid3-W5 and Pid3-I1. Amino acid sequences alignment of these orthologs indicated that the sequence diversities between the blast resistance orthologs were mostly located in the LRR domain such as the substitutions of Q694H,D856H,Q896R,D899E etc. However, the differences between the resistance orthologs and the susceptible ones were mostly located in the NBS domain. The present experiments provide an example of that the ortholog evaluation of plant R genes could be an efficient way to expand the rice blast resistance and some other plant disease resistance as well for breeding. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24681716/Excavation_of_Pid3_orthologs_with_differential_resistance_spectra_to_Magnaporthe_oryzae_in_rice_resource_ L2 - https://dx.plos.org/10.1371/journal.pone.0093275 DB - PRIME DP - Unbound Medicine ER -