Tags

Type your tag names separated by a space and hit enter

Effects of intraperitoneal melatonin on caustic sclerosing cholangitis due to scolicidal solution in a rat model.

Abstract

BACKGROUND

Hydatid disease is a worldwide health problem. Treatment is surgical or percutaneous, using scolicidal agents. Caustic sclerosing cholangitis might develop after the contact of scolicidal agents with the biliary ducts. Melatonin, an antioxidant, anti-inflammatory, and anticarcinogenic agent, might be used in the treatment of caustic sclerosing cholangitis due to its possible preventive effects on fibrosis and cell damage.

OBJECTIVE

The aim of the study was to investigate the effects of melatonin on an experimentally developed caustic sclerosing cholangitis with scolicidal solution (formalin) in a rat model.

METHODS

Forty female Sprague-Dawley rats aged 11 to 13 weeks and weighing 250 ± 30 g were randomly assigned to 1 of 4 groups of 10: formalin 5% at 0.5 mL/d + melatonin placebo; formalin placebo + intraperitoneal melatonin 10 mg/kg/d; formalin 5% at 0.5 mL/d + melatonin 10 mg/kg/d; and formalin placebo and melatonin placebo (control). Hepatobiliary function was assessed using dynamic scintigraphy with technetium-99m-mebrofenin on study day 60. The histology of the liver and biliary duct specimens was examined on study day 60. In each group, histopathologic alterations were scored as absent, slight, mild, or severe.

RESULTS

Mean severity scores for parenchymal necrosis in the liver (P < 0.01), portal fibrosis (P < 0.01), biliary duct proliferation (P < 0.001), cholangitis/ pericholangitis (P < 0.01), hyperemia in the biliary ducts (P < 0.01), and fibrosis (P < 0.01) were significantly lower in rats treated with formalin + melatonin compared with those treated with formalin alone. No significant differences were observed between the 3 treatment groups with respect to t½, a parameter used to assess the secretion function of the hepatocytes. However, the t½ was significantly longer in the treatment groups compared with controls (P < 0.001).

CONCLUSION

In this experimental study in a rat model of caustic sclerosing cholangitis, the histopathologic and scintigraphic findings suggested that melatonin is effective in attenuating the damage caused by scolicidal agents on the liver and biliary ducts.

Links

  • PMC Free PDF
  • PMC Free Full Text
  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.

    ,

    Department of General Surgery, Faculty of Medicine, Trakya University, Edirne, Turkey.

    ,

    Department of Pathology, Faculty of Medicine, Trakya University, Edirne, Turkey.

    ,

    Department of Nuclear Medicine, Faculty of Medicine, Trakya University, Edirne, Turkey.

    Department of Biostatistics, Faculty of Medicine, Trakya University, Edirne, Turkey.

    Source

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    24683258

    Citation

    Sezer, Atakan, et al. "Effects of Intraperitoneal Melatonin On Caustic Sclerosing Cholangitis Due to Scolicidal Solution in a Rat Model." Current Therapeutic Research, Clinical and Experimental, vol. 71, no. 2, 2010, pp. 118-28.
    Sezer A, Hatipoglu AR, Usta U, et al. Effects of intraperitoneal melatonin on caustic sclerosing cholangitis due to scolicidal solution in a rat model. Curr Ther Res Clin Exp. 2010;71(2):118-28.
    Sezer, A., Hatipoglu, A. R., Usta, U., Altun, G., & Sut, N. (2010). Effects of intraperitoneal melatonin on caustic sclerosing cholangitis due to scolicidal solution in a rat model. Current Therapeutic Research, Clinical and Experimental, 71(2), pp. 118-28. doi:10.1016/j.curtheres.2010.03.004.
    Sezer A, et al. Effects of Intraperitoneal Melatonin On Caustic Sclerosing Cholangitis Due to Scolicidal Solution in a Rat Model. Curr Ther Res Clin Exp. 2010;71(2):118-28. PubMed PMID: 24683258.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Effects of intraperitoneal melatonin on caustic sclerosing cholangitis due to scolicidal solution in a rat model. AU - Sezer,Atakan, AU - Hatipoglu,Ahmet Rahmi, AU - Usta,Ufuk, AU - Altun,Gülay, AU - Sut,Necdet, PY - 2010/01/14/accepted PY - 2014/4/1/entrez PY - 2010/4/1/pubmed PY - 2010/4/1/medline KW - caustic sclerosing cholangitis KW - hydatid cyst KW - melatonin KW - scolicidal solutions SP - 118 EP - 28 JF - Current therapeutic research, clinical and experimental JO - Curr Ther Res Clin Exp VL - 71 IS - 2 N2 - BACKGROUND: Hydatid disease is a worldwide health problem. Treatment is surgical or percutaneous, using scolicidal agents. Caustic sclerosing cholangitis might develop after the contact of scolicidal agents with the biliary ducts. Melatonin, an antioxidant, anti-inflammatory, and anticarcinogenic agent, might be used in the treatment of caustic sclerosing cholangitis due to its possible preventive effects on fibrosis and cell damage. OBJECTIVE: The aim of the study was to investigate the effects of melatonin on an experimentally developed caustic sclerosing cholangitis with scolicidal solution (formalin) in a rat model. METHODS: Forty female Sprague-Dawley rats aged 11 to 13 weeks and weighing 250 ± 30 g were randomly assigned to 1 of 4 groups of 10: formalin 5% at 0.5 mL/d + melatonin placebo; formalin placebo + intraperitoneal melatonin 10 mg/kg/d; formalin 5% at 0.5 mL/d + melatonin 10 mg/kg/d; and formalin placebo and melatonin placebo (control). Hepatobiliary function was assessed using dynamic scintigraphy with technetium-99m-mebrofenin on study day 60. The histology of the liver and biliary duct specimens was examined on study day 60. In each group, histopathologic alterations were scored as absent, slight, mild, or severe. RESULTS: Mean severity scores for parenchymal necrosis in the liver (P < 0.01), portal fibrosis (P < 0.01), biliary duct proliferation (P < 0.001), cholangitis/ pericholangitis (P < 0.01), hyperemia in the biliary ducts (P < 0.01), and fibrosis (P < 0.01) were significantly lower in rats treated with formalin + melatonin compared with those treated with formalin alone. No significant differences were observed between the 3 treatment groups with respect to t½, a parameter used to assess the secretion function of the hepatocytes. However, the t½ was significantly longer in the treatment groups compared with controls (P < 0.001). CONCLUSION: In this experimental study in a rat model of caustic sclerosing cholangitis, the histopathologic and scintigraphic findings suggested that melatonin is effective in attenuating the damage caused by scolicidal agents on the liver and biliary ducts. SN - 0011-393X UR - https://www.unboundmedicine.com/medline/citation/24683258/Effects_of_intraperitoneal_melatonin_on_caustic_sclerosing_cholangitis_due_to_scolicidal_solution_in_a_rat_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0011-393X(10)00038-X DB - PRIME DP - Unbound Medicine ER -