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Rapid and high-throughput determination of endogenous cytokinins in Oryza sativa by bare Fe3O4 nanoparticles-based magnetic solid-phase extraction.
J Chromatogr A. 2014 May 02; 1340:146-50.JC

Abstract

A rapid method was developed for determination of endogenous cytokinins (CKs) based on magnetic solid-phase extraction (MSPE) followed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). We illustrated the hydrophilic character of bare Fe3O4 nanoparticles that were directly used as a MSPE sorbent for rapid enrichment of endogenous CKs from complex plant extract. To the best of our knowledge, this is the first report of bare Fe3O4 directly used as efficient extraction sorbent to enrich target CKs based on hydrophilic interaction. Under the optimized conditions, a rapid, sensitive and high-throughput method for the determination of 16 CKs was established by combination of MSPE with UPLC-MS/MS. Good linearity was obtained with correlation coefficients (r) from 0.9902 to 0.9998. The limits of detection (LODs) and quantification (LOQs) ranged from 1.2 pg mL(-1) to 391.3 pg mL(-1) and 4.1 pg mL(-1) to 1304.3 pg mL(-1), respectively. 16 CKs could be successfully determined in spiked sample with 80.6-117.3% recoveries and the relative standard deviations (RSDs) were less than 16.6%. Finally, 10 endogenous CKs were successfully quantified in 50mg Oryza sativa sample using the developed MSPE-UPLC-MS/MS method.

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Authors+Show Affiliations

Cai BD
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Zhu JX
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Gao Q
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China; Engineering Research Center of Nano-Geomaterials of Ministry of Education, Department of Chemistry, China University of Geosciences, Wuhan 430074, China.
Luo D
Shimadzu Global COE for Application & Technical Development, Shimadzu International Trading (Shanghai) Co., Ltd, Shanghai 200052, China.
Yuan BF
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.
Feng YQ
Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China. Electronic address: yqfeng@whu.edu.cn.

MeSH

Chromatography, LiquidCytokininsFerric CompoundsHydrophobic and Hydrophilic InteractionsLimit of DetectionMagnetite NanoparticlesOryzaSolid Phase ExtractionTandem Mass Spectrometry

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24685168

Citation

Cai, Bao-Dong, et al. "Rapid and High-throughput Determination of Endogenous Cytokinins in Oryza Sativa By Bare Fe3O4 Nanoparticles-based Magnetic Solid-phase Extraction." Journal of Chromatography. A, vol. 1340, 2014, pp. 146-50.
Cai BD, Zhu JX, Gao Q, et al. Rapid and high-throughput determination of endogenous cytokinins in Oryza sativa by bare Fe3O4 nanoparticles-based magnetic solid-phase extraction. J Chromatogr A. 2014;1340:146-50.
Cai, B. D., Zhu, J. X., Gao, Q., Luo, D., Yuan, B. F., & Feng, Y. Q. (2014). Rapid and high-throughput determination of endogenous cytokinins in Oryza sativa by bare Fe3O4 nanoparticles-based magnetic solid-phase extraction. Journal of Chromatography. A, 1340, 146-50. https://doi.org/10.1016/j.chroma.2014.03.030
Cai BD, et al. Rapid and High-throughput Determination of Endogenous Cytokinins in Oryza Sativa By Bare Fe3O4 Nanoparticles-based Magnetic Solid-phase Extraction. J Chromatogr A. 2014 May 2;1340:146-50. PubMed PMID: 24685168.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid and high-throughput determination of endogenous cytokinins in Oryza sativa by bare Fe3O4 nanoparticles-based magnetic solid-phase extraction. AU - Cai,Bao-Dong, AU - Zhu,Jiu-Xia, AU - Gao,Qiang, AU - Luo,Dan, AU - Yuan,Bi-Feng, AU - Feng,Yu-Qi, Y1 - 2014/03/18/ PY - 2013/11/14/received PY - 2014/03/10/revised PY - 2014/03/11/accepted PY - 2014/4/2/entrez PY - 2014/4/2/pubmed PY - 2014/8/17/medline KW - Cytokinins KW - Fe(3)O(4) nanoparticles KW - Hydrophilic interaction KW - Magnetic solid-phase extraction KW - UPLC-MS/MS SP - 146 EP - 50 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1340 N2 - A rapid method was developed for determination of endogenous cytokinins (CKs) based on magnetic solid-phase extraction (MSPE) followed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). We illustrated the hydrophilic character of bare Fe3O4 nanoparticles that were directly used as a MSPE sorbent for rapid enrichment of endogenous CKs from complex plant extract. To the best of our knowledge, this is the first report of bare Fe3O4 directly used as efficient extraction sorbent to enrich target CKs based on hydrophilic interaction. Under the optimized conditions, a rapid, sensitive and high-throughput method for the determination of 16 CKs was established by combination of MSPE with UPLC-MS/MS. Good linearity was obtained with correlation coefficients (r) from 0.9902 to 0.9998. The limits of detection (LODs) and quantification (LOQs) ranged from 1.2 pg mL(-1) to 391.3 pg mL(-1) and 4.1 pg mL(-1) to 1304.3 pg mL(-1), respectively. 16 CKs could be successfully determined in spiked sample with 80.6-117.3% recoveries and the relative standard deviations (RSDs) were less than 16.6%. Finally, 10 endogenous CKs were successfully quantified in 50mg Oryza sativa sample using the developed MSPE-UPLC-MS/MS method. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/24685168/Rapid_and_high_throughput_determination_of_endogenous_cytokinins_in_Oryza_sativa_by_bare_Fe3O4_nanoparticles_based_magnetic_solid_phase_extraction_ DB - PRIME DP - Unbound Medicine ER -
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