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Application of quantitative 1H-NMR method to determination of gentiopicroside in Gentianae radix and Gentianae scabrae radix.
J Nat Med. 2014 Jul; 68(3):630-5.JN

Abstract

A quantitative (1)H-NMR method (qHNMR) was used to measure gentiopicroside content in Gentianae radix and Gentianae scabrae radix. Gentiopicroside is a major component of Gentianae radix and Gentianae scabrae radix. The purity of gentiopicroside was calculated from the ratio of the intensity of the H-3 signal at δ 7.44 ppm or the H-8 signal at δ 5.78 ppm in methanol-d 4 of gentiopicroside to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material (CRM) grade. As a result, the gentiopicroside content in two lots of Gentianae radix as determined by qHNMR was found to be 1.76 and 2.17 %, respectively. The gentiopicroside content in two lots of Gentianae scabrae radix was 2.73 and 3.99 %, respectively. We demonstrated that this method is useful for the quantitative analysis of crude drugs.

Authors+Show Affiliations

Laboratory of Pharmacognosy, College of Pharmacy, Kinjo Gakuin University, 2-1723 Omori, Moriyama-ku, Nagoya, 463-8521, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24687868

Citation

Tanaka, Rie, et al. "Application of Quantitative 1H-NMR Method to Determination of Gentiopicroside in Gentianae Radix and Gentianae Scabrae Radix." Journal of Natural Medicines, vol. 68, no. 3, 2014, pp. 630-5.
Tanaka R, Hasebe Y, Nagatsu A. Application of quantitative 1H-NMR method to determination of gentiopicroside in Gentianae radix and Gentianae scabrae radix. J Nat Med. 2014;68(3):630-5.
Tanaka, R., Hasebe, Y., & Nagatsu, A. (2014). Application of quantitative 1H-NMR method to determination of gentiopicroside in Gentianae radix and Gentianae scabrae radix. Journal of Natural Medicines, 68(3), 630-5. https://doi.org/10.1007/s11418-014-0833-0
Tanaka R, Hasebe Y, Nagatsu A. Application of Quantitative 1H-NMR Method to Determination of Gentiopicroside in Gentianae Radix and Gentianae Scabrae Radix. J Nat Med. 2014;68(3):630-5. PubMed PMID: 24687868.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Application of quantitative 1H-NMR method to determination of gentiopicroside in Gentianae radix and Gentianae scabrae radix. AU - Tanaka,Rie, AU - Hasebe,Yuko, AU - Nagatsu,Akito, Y1 - 2014/04/01/ PY - 2014/02/25/received PY - 2014/03/17/accepted PY - 2014/4/2/entrez PY - 2014/4/2/pubmed PY - 2014/8/20/medline SP - 630 EP - 5 JF - Journal of natural medicines JO - J Nat Med VL - 68 IS - 3 N2 - A quantitative (1)H-NMR method (qHNMR) was used to measure gentiopicroside content in Gentianae radix and Gentianae scabrae radix. Gentiopicroside is a major component of Gentianae radix and Gentianae scabrae radix. The purity of gentiopicroside was calculated from the ratio of the intensity of the H-3 signal at δ 7.44 ppm or the H-8 signal at δ 5.78 ppm in methanol-d 4 of gentiopicroside to that of a hexamethyldisilane (HMD) signal at 0 ppm. The concentration of HMD was corrected with SI traceability by using potassium hydrogen phthalate of certified reference material (CRM) grade. As a result, the gentiopicroside content in two lots of Gentianae radix as determined by qHNMR was found to be 1.76 and 2.17 %, respectively. The gentiopicroside content in two lots of Gentianae scabrae radix was 2.73 and 3.99 %, respectively. We demonstrated that this method is useful for the quantitative analysis of crude drugs. SN - 1861-0293 UR - https://www.unboundmedicine.com/medline/citation/24687868/Application_of_quantitative_1H_NMR_method_to_determination_of_gentiopicroside_in_Gentianae_radix_and_Gentianae_scabrae_radix_ DB - PRIME DP - Unbound Medicine ER -