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Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.).
Biomed Res Int. 2014; 2014:918209.BR

Abstract

Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%), α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as 11.47 ± 0.05, 10.88 ± 0.9, 9.68 ± 0.06, and 8.33 ± 0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition against Fusarium moniliforme in inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria.

Authors+Show Affiliations

Chemistry Department, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh 273009, India.Chemistry Department, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh 273009, India.Chemistry Department, DDU Gorakhpur University, Gorakhpur, Uttar Pradesh 273009, India.INQUINOA-CONICET, Instituto de Química Orgánica, Facultad de Bioquímica Química y Farmacia, Universidad Nacional de Tucumán, T4000INI San Miguel de Tucumán, Argentina.INQUINOA-CONICET, Instituto de Química Orgánica, Facultad de Bioquímica Química y Farmacia, Universidad Nacional de Tucumán, T4000INI San Miguel de Tucumán, Argentina.INQUINOA-CONICET, Instituto de Química Orgánica, Facultad de Bioquímica Química y Farmacia, Universidad Nacional de Tucumán, T4000INI San Miguel de Tucumán, Argentina.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24689064

Citation

Singh, Sunita, et al. "Composition, in Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained From Black Cumin Seeds (Nigella Sativa L.)." BioMed Research International, vol. 2014, 2014, p. 918209.
Singh S, Das SS, Singh G, et al. Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.). Biomed Res Int. 2014;2014:918209.
Singh, S., Das, S. S., Singh, G., Schuff, C., de Lampasona, M. P., & Catalán, C. A. (2014). Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.). BioMed Research International, 2014, 918209. https://doi.org/10.1155/2014/918209
Singh S, et al. Composition, in Vitro Antioxidant and Antimicrobial Activities of Essential Oil and Oleoresins Obtained From Black Cumin Seeds (Nigella Sativa L.). Biomed Res Int. 2014;2014:918209. PubMed PMID: 24689064.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Composition, in vitro antioxidant and antimicrobial activities of essential oil and oleoresins obtained from black cumin seeds (Nigella sativa L.). AU - Singh,Sunita, AU - Das,S S, AU - Singh,G, AU - Schuff,Carola, AU - de Lampasona,Marina P, AU - Catalán,César A N, Y1 - 2014/02/06/ PY - 2013/04/06/received PY - 2013/10/24/accepted PY - 2014/4/2/entrez PY - 2014/4/2/pubmed PY - 2015/5/15/medline SP - 918209 EP - 918209 JF - BioMed research international JO - Biomed Res Int VL - 2014 N2 - Gas chromatography-mass spectrometry (GC-MS) analysis revealed the major components in black cumin essential oils which were thymoquinone (37.6%) followed by p-cymene (31.2%), α-thujene (5.6%), thymohydroquinone (3.4%), and longifolene (2.0%), whereas the oleoresins extracted in different solvents contain linoleic acid as a major component. The antioxidant activity of essential oil and oleoresins was evaluated against linseed oil system at 200 ppm concentration by peroxide value, thiobarbituric acid value, ferric thiocyanate, ferrous ion chelating activity, and 1,1-diphenyl-2-picrylhydrazyl radical scavenging methods. The essential oil and ethyl acetate oleoresin were found to be better than synthetic antioxidants. The total phenol contents (gallic acid equivalents, mg GAE per g) in black cumin essential oil, ethyl acetate, ethanol, and n-hexane oleoresins were calculated as 11.47 ± 0.05, 10.88 ± 0.9, 9.68 ± 0.06, and 8.33 ± 0.01, respectively, by Folin-Ciocalteau method. The essential oil showed up to 90% zone inhibition against Fusarium moniliforme in inverted petri plate method. Using agar well diffusion method for evaluating antibacterial activity, the essential oil was found to be highly effective against Gram-positive bacteria. SN - 2314-6141 UR - https://www.unboundmedicine.com/medline/citation/24689064/Composition_in_vitro_antioxidant_and_antimicrobial_activities_of_essential_oil_and_oleoresins_obtained_from_black_cumin_seeds__Nigella_sativa_L___ L2 - https://doi.org/10.1155/2014/918209 DB - PRIME DP - Unbound Medicine ER -