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Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone.
Cell Tissue Bank 2015; 16(1):73-81CT

Abstract

Thermodisinfection of human femoral heads from living donors harvested during hip joint replacement is an established processing procedure. This study was designed to examine the influence of heat sterilization on pull out strength of cancellous bone and storage at different temperatures up to 2 years since we had previously studied the storage of unprocessed cancellous bone. Porcine cancellous bone resembling human bone structure was obtained from 140 proximal humerus of 6-8 months old piglets. Pull out strength of screws after thermodisinfection was compared with unprocessed cancellous bone and tested immediately and after 6, 12 and 24 months of storage at -20 and -80 °C. A three-way ANOVA was performed and significance level was 5 %. The thermodisinfected bone showed a pull out force of 2729 N (1657-3568 N). The reduction of pull out strength compared with unprocessed bone over all periods of storage was 276 N on average with 95 % confidence interval ranging from 166 N to 389 N (p < 0.0001). Different freezing temperatures did not influence this mechanic property within 24 months storage and showed no difference compared with fresh frozen bone. Thermodisinfection of cancellous bone preserves tensile strength necessary for clinical purposes. The storage at -20 °C for at least 2 years did not show relevant decrease of pull out strength compared with -80 °C without difference between thermodisinfected and fresh frozen bone. The increase of the storage temperature to -20 °C for at least 2 years should be considered.

Authors+Show Affiliations

Department of Orthopaedic Surgery, Philipps University Medical School, Philipps University Marburg, Baldingerstrasse, 35043, Marburg, Germany, christian.foelsch@t-online.de.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24692177

Citation

Fölsch, Christian, et al. "Influence of Thermodisinfection and Duration of Cryopreservation at Different Temperatures On Pull Out Strength of Cancellous Bone." Cell and Tissue Banking, vol. 16, no. 1, 2015, pp. 73-81.
Fölsch C, Mittelmeier W, von Garrel T, et al. Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone. Cell Tissue Bank. 2015;16(1):73-81.
Fölsch, C., Mittelmeier, W., von Garrel, T., Bilderbeek, U., Timmesfeld, N., Pruss, A., & Matter, H. P. (2015). Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone. Cell and Tissue Banking, 16(1), pp. 73-81. doi:10.1007/s10561-014-9442-0.
Fölsch C, et al. Influence of Thermodisinfection and Duration of Cryopreservation at Different Temperatures On Pull Out Strength of Cancellous Bone. Cell Tissue Bank. 2015;16(1):73-81. PubMed PMID: 24692177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Influence of thermodisinfection and duration of cryopreservation at different temperatures on pull out strength of cancellous bone. AU - Fölsch,Christian, AU - Mittelmeier,Wolfram, AU - von Garrel,Thomas, AU - Bilderbeek,Uwe, AU - Timmesfeld,Nina, AU - Pruss,Axel, AU - Matter,Hans-Peter, Y1 - 2014/04/02/ PY - 2014/01/02/received PY - 2014/03/21/accepted PY - 2014/4/3/entrez PY - 2014/4/3/pubmed PY - 2015/12/15/medline SP - 73 EP - 81 JF - Cell and tissue banking JO - Cell Tissue Bank VL - 16 IS - 1 N2 - Thermodisinfection of human femoral heads from living donors harvested during hip joint replacement is an established processing procedure. This study was designed to examine the influence of heat sterilization on pull out strength of cancellous bone and storage at different temperatures up to 2 years since we had previously studied the storage of unprocessed cancellous bone. Porcine cancellous bone resembling human bone structure was obtained from 140 proximal humerus of 6-8 months old piglets. Pull out strength of screws after thermodisinfection was compared with unprocessed cancellous bone and tested immediately and after 6, 12 and 24 months of storage at -20 and -80 °C. A three-way ANOVA was performed and significance level was 5 %. The thermodisinfected bone showed a pull out force of 2729 N (1657-3568 N). The reduction of pull out strength compared with unprocessed bone over all periods of storage was 276 N on average with 95 % confidence interval ranging from 166 N to 389 N (p < 0.0001). Different freezing temperatures did not influence this mechanic property within 24 months storage and showed no difference compared with fresh frozen bone. Thermodisinfection of cancellous bone preserves tensile strength necessary for clinical purposes. The storage at -20 °C for at least 2 years did not show relevant decrease of pull out strength compared with -80 °C without difference between thermodisinfected and fresh frozen bone. The increase of the storage temperature to -20 °C for at least 2 years should be considered. SN - 1573-6814 UR - https://www.unboundmedicine.com/medline/citation/24692177/Influence_of_thermodisinfection_and_duration_of_cryopreservation_at_different_temperatures_on_pull_out_strength_of_cancellous_bone_ L2 - https://doi.org/10.1007/s10561-014-9442-0 DB - PRIME DP - Unbound Medicine ER -