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Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease.
Mol Neurodegener 2014; 9:13MN

Abstract

BACKGROUND

According to the modified amyloid hypothesis the main event in the pathogenesis of Alzheimer's disease (AD) is the deposition of neurotoxic amyloid β-peptide (Aβ) within neurons. Additionally to full-length peptides, a great diversity of N-truncated Aβ variants is derived from the larger amyloid precursor protein (APP). Vast evidence suggests that Aβx₋₄₂ isoforms play an important role triggering neurodegeneration due to its high abundance, amyloidogenic propensity and toxicity. Although N-truncated and Aβx₋₄₂ species have been pointed as crucial players in AD etiology, the Aβ₅-x isoforms have not received much attention.

RESULTS

The present study is the first to show immunohistochemical evidence of Aβ₅-x in familial cases of AD (FAD) and its distribution in APP/PS1KI, 5XFAD and 3xTG transgenic mouse models. In order to probe Aβ₅-x peptides we generated the AB5-3 antibody. Positive plaques and congophilic amyloid angiopathy (CAA) were observed among all the FAD cases tested carrying either APP or presenilin 1 (PS1) mutations and most of the sporadic cases of AD (SAD). Different patterns of Aβ5-x distribution were found in the mouse models carrying different combinations of autosomal mutations in the APP, PS1 and Tau genes. All of them showed extracellular Aβ deposits but none CAA. Additionally, they were all affected by a severe amyloid pathology in the hippocampus among other areas. Interestingly, neither 5XFAD nor APP/PS1KI showed any evidence for intraneuronal Aβ₅-x.

CONCLUSIONS

Different degrees of Aβ₅-x accumulations can be found in the transgenic AD mouse models and human cases expressing the sporadic or the familial form of the disease. Due to the lack of intracellular Aβ₅-x, these isoforms might not be contributing to early mechanisms in the cascade of events triggering AD pathology. Brain sections obtained from SAD cases showed higher Aβ₅-x-immunoreactivity in vascular deposits than in extracellular plaques, while both are equally important in the FAD cases. The difference may rely on alternative mechanisms involving Aβ₅-x peptides and operating in a divergent way in the late and early onset forms of the disease.

Authors+Show Affiliations

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableDivision of Molecular Psychiatry Department of Psychiatry, University Medicine Goettingen, D-37075, von-Siebold-Str, 5, Goettingen 37075, Germany. tbayer@gwdg.de.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24694184

Citation

Guzmán, Erika Avendaño, et al. "Abundance of Aβ₅-x Like Immunoreactivity in Transgenic 5XFAD, APP/PS1KI and 3xTG Mice, Sporadic and Familial Alzheimer's Disease." Molecular Neurodegeneration, vol. 9, 2014, p. 13.
Guzmán EA, Bouter Y, Richard BC, et al. Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease. Mol Neurodegener. 2014;9:13.
Guzmán, E. A., Bouter, Y., Richard, B. C., Lannfelt, L., Ingelsson, M., Paetau, A., ... Bayer, T. A. (2014). Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease. Molecular Neurodegeneration, 9, p. 13. doi:10.1186/1750-1326-9-13.
Guzmán EA, et al. Abundance of Aβ₅-x Like Immunoreactivity in Transgenic 5XFAD, APP/PS1KI and 3xTG Mice, Sporadic and Familial Alzheimer's Disease. Mol Neurodegener. 2014 Apr 2;9:13. PubMed PMID: 24694184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Abundance of Aβ₅-x like immunoreactivity in transgenic 5XFAD, APP/PS1KI and 3xTG mice, sporadic and familial Alzheimer's disease. AU - Guzmán,Erika Avendaño, AU - Bouter,Yvonne, AU - Richard,Bernhard C, AU - Lannfelt,Lars, AU - Ingelsson,Martin, AU - Paetau,Anders, AU - Verkkoniemi-Ahola,Auli, AU - Wirths,Oliver, AU - Bayer,Thomas A, Y1 - 2014/04/02/ PY - 2014/02/11/received PY - 2014/03/28/accepted PY - 2014/4/4/entrez PY - 2014/4/4/pubmed PY - 2014/9/13/medline SP - 13 EP - 13 JF - Molecular neurodegeneration JO - Mol Neurodegener VL - 9 N2 - BACKGROUND: According to the modified amyloid hypothesis the main event in the pathogenesis of Alzheimer's disease (AD) is the deposition of neurotoxic amyloid β-peptide (Aβ) within neurons. Additionally to full-length peptides, a great diversity of N-truncated Aβ variants is derived from the larger amyloid precursor protein (APP). Vast evidence suggests that Aβx₋₄₂ isoforms play an important role triggering neurodegeneration due to its high abundance, amyloidogenic propensity and toxicity. Although N-truncated and Aβx₋₄₂ species have been pointed as crucial players in AD etiology, the Aβ₅-x isoforms have not received much attention. RESULTS: The present study is the first to show immunohistochemical evidence of Aβ₅-x in familial cases of AD (FAD) and its distribution in APP/PS1KI, 5XFAD and 3xTG transgenic mouse models. In order to probe Aβ₅-x peptides we generated the AB5-3 antibody. Positive plaques and congophilic amyloid angiopathy (CAA) were observed among all the FAD cases tested carrying either APP or presenilin 1 (PS1) mutations and most of the sporadic cases of AD (SAD). Different patterns of Aβ5-x distribution were found in the mouse models carrying different combinations of autosomal mutations in the APP, PS1 and Tau genes. All of them showed extracellular Aβ deposits but none CAA. Additionally, they were all affected by a severe amyloid pathology in the hippocampus among other areas. Interestingly, neither 5XFAD nor APP/PS1KI showed any evidence for intraneuronal Aβ₅-x. CONCLUSIONS: Different degrees of Aβ₅-x accumulations can be found in the transgenic AD mouse models and human cases expressing the sporadic or the familial form of the disease. Due to the lack of intracellular Aβ₅-x, these isoforms might not be contributing to early mechanisms in the cascade of events triggering AD pathology. Brain sections obtained from SAD cases showed higher Aβ₅-x-immunoreactivity in vascular deposits than in extracellular plaques, while both are equally important in the FAD cases. The difference may rely on alternative mechanisms involving Aβ₅-x peptides and operating in a divergent way in the late and early onset forms of the disease. SN - 1750-1326 UR - https://www.unboundmedicine.com/medline/citation/24694184/Abundance_of_Aβ₅_x_like_immunoreactivity_in_transgenic_5XFAD_APP/PS1KI_and_3xTG_mice_sporadic_and_familial_Alzheimer's_disease_ L2 - https://molecularneurodegeneration.biomedcentral.com/articles/10.1186/1750-1326-9-13 DB - PRIME DP - Unbound Medicine ER -