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Head-to-head comparison of second-generation nucleic acid amplification tests for detection of Chlamydia trachomatis and Neisseria gonorrhoeae on urine samples from female subjects and self-collected vaginal swabs.
J Clin Microbiol. 2014 Jul; 52(7):2305-10.JC

Abstract

In a comparison of 4 second-generation nucleic acid amplification tests performed with self-collected vaginal swab (SCVS) and first-void urine (FVU) specimens from 575 women, SCVS specimens indicated more infections than did FVU specimens in all assays. The prevalence rates were 9% (53/575 patients) for Chlamydia trachomatis and 2% (11/575 patients) for Neisseria gonorrhoeae. The clinical sensitivities for testing SCVS specimens for C. trachomatis were 98.1% on a Tigris system and 96.2% on a Panther system for the Aptima Combo 2 assay (Hologic Gen-Probe), 98.0% for the RealTime CT/NG assay on an m2000 instrument (Abbott), 90.6% for the ProbeTec CT/GC Q(x) assay on the Viper system (Becton Dickinson), and 84.6% for the cobas CT/NG assay on the cobas 4800 platform (Roche). Clinical sensitivities for C. trachomatis in FVU specimens were 88.7% (Tigris) and 88.0% (Panther) for the Aptima Combo 2 assay, 76.9% for the RealTime CT/NG assay, 75.5% for the ProbeTec CT/GC Q(x) assay, and 81.1% for the cobas CT/NG assay. Clinical sensitivities of the assays for N. gonorrhoeae, with limited positive results, ranged from 63.6% to 100%. Specificities for both infections ranged from 98.4 to 100%. Differences in analytical sensitivities and levels of molecular targets in clinical samples but not inhibitors of amplification may explain the differences in clinical sensitivities.

Authors+Show Affiliations

St. Joseph's Healthcare/McMaster University, Hamilton, Ontario, Canada chernesk@mcmaster.ca.St. Joseph's Healthcare/McMaster University, Hamilton, Ontario, Canada.St. Joseph's Healthcare/McMaster University, Hamilton, Ontario, Canada.Queen Elizabeth II Health Sciences Centre, Dalhousie University, Halifax, Nova Scotia, Canada.Centre de santé et de services sociaux de Trois-Rivières, Trois-Rivières, Quebec, Canada.Public Health Laboratory, St. John's, Newfoundland and Labrador, Canada.St. Joseph's Healthcare/McMaster University, Hamilton, Ontario, Canada.Division of Community Health and Humanities, Memorial University, St. John's, Newfoundland and Labrador, Canada Department of Oncology, McGill University, Montreal, Quebec, Canada.

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24696024

Citation

Chernesky, Max, et al. "Head-to-head Comparison of Second-generation Nucleic Acid Amplification Tests for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae On Urine Samples From Female Subjects and Self-collected Vaginal Swabs." Journal of Clinical Microbiology, vol. 52, no. 7, 2014, pp. 2305-10.
Chernesky M, Jang D, Gilchrist J, et al. Head-to-head comparison of second-generation nucleic acid amplification tests for detection of Chlamydia trachomatis and Neisseria gonorrhoeae on urine samples from female subjects and self-collected vaginal swabs. J Clin Microbiol. 2014;52(7):2305-10.
Chernesky, M., Jang, D., Gilchrist, J., Hatchette, T., Poirier, A., Flandin, J. F., Smieja, M., & Ratnam, S. (2014). Head-to-head comparison of second-generation nucleic acid amplification tests for detection of Chlamydia trachomatis and Neisseria gonorrhoeae on urine samples from female subjects and self-collected vaginal swabs. Journal of Clinical Microbiology, 52(7), 2305-10. https://doi.org/10.1128/JCM.03552-13
Chernesky M, et al. Head-to-head Comparison of Second-generation Nucleic Acid Amplification Tests for Detection of Chlamydia Trachomatis and Neisseria Gonorrhoeae On Urine Samples From Female Subjects and Self-collected Vaginal Swabs. J Clin Microbiol. 2014;52(7):2305-10. PubMed PMID: 24696024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Head-to-head comparison of second-generation nucleic acid amplification tests for detection of Chlamydia trachomatis and Neisseria gonorrhoeae on urine samples from female subjects and self-collected vaginal swabs. AU - Chernesky,Max, AU - Jang,Dan, AU - Gilchrist,Jodi, AU - Hatchette,Todd, AU - Poirier,André, AU - Flandin,Jean-Frederic, AU - Smieja,Marek, AU - Ratnam,Sam, Y1 - 2014/04/02/ PY - 2014/4/4/entrez PY - 2014/4/4/pubmed PY - 2015/2/14/medline SP - 2305 EP - 10 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 52 IS - 7 N2 - In a comparison of 4 second-generation nucleic acid amplification tests performed with self-collected vaginal swab (SCVS) and first-void urine (FVU) specimens from 575 women, SCVS specimens indicated more infections than did FVU specimens in all assays. The prevalence rates were 9% (53/575 patients) for Chlamydia trachomatis and 2% (11/575 patients) for Neisseria gonorrhoeae. The clinical sensitivities for testing SCVS specimens for C. trachomatis were 98.1% on a Tigris system and 96.2% on a Panther system for the Aptima Combo 2 assay (Hologic Gen-Probe), 98.0% for the RealTime CT/NG assay on an m2000 instrument (Abbott), 90.6% for the ProbeTec CT/GC Q(x) assay on the Viper system (Becton Dickinson), and 84.6% for the cobas CT/NG assay on the cobas 4800 platform (Roche). Clinical sensitivities for C. trachomatis in FVU specimens were 88.7% (Tigris) and 88.0% (Panther) for the Aptima Combo 2 assay, 76.9% for the RealTime CT/NG assay, 75.5% for the ProbeTec CT/GC Q(x) assay, and 81.1% for the cobas CT/NG assay. Clinical sensitivities of the assays for N. gonorrhoeae, with limited positive results, ranged from 63.6% to 100%. Specificities for both infections ranged from 98.4 to 100%. Differences in analytical sensitivities and levels of molecular targets in clinical samples but not inhibitors of amplification may explain the differences in clinical sensitivities. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/24696024/Head_to_head_comparison_of_second_generation_nucleic_acid_amplification_tests_for_detection_of_Chlamydia_trachomatis_and_Neisseria_gonorrhoeae_on_urine_samples_from_female_subjects_and_self_collected_vaginal_swabs_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=24696024 DB - PRIME DP - Unbound Medicine ER -