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Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe.
Mol Genet Genomics. 2014 Aug; 289(4):685-93.MG

Abstract

The Ecl1 family genes extend the lifespan of fission yeast when overexpressed. They also cause resistance against H(2)O(2) stress. In this study, we found that the bZip transcription factor Atf1 is a direct activator of the induction of extender of chronological lifespan (ecl1 (+)) by H(2)O(2) stress. Based on ChIP analysis, we identified that Atf1 binds to the upstream DNA region of ecl1(+). Previously, we reported that overexpression of ecl1(+) increased the expression of the catalase-encoding ctt1(+). This ecl1(+)-dependent increase of ctt1(+) expression occurred in ∆atf1 mutant. On the other hand, the activation of ctt1 (+) caused by the ∆pyp1 mutation, which enhances Sty1-Atf1 activity, could occur in ∆ecl1 mutant. Based on these results, we propose that Atf1 can regulate ctt1(+) in both an Ecl1-dependent and an Ecl1-independent manner.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Department of Basic Medicinal Sciences, Graduate School of Pharmaceutical Sciences, Nagoya University, Chikusa-ku, Nagoya, 464-8601, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24696293

Citation

Shimasaki, Takafumi, et al. "Ecl1 Is Activated By the Transcription Factor Atf1 in Response to H2O2 Stress in Schizosaccharomyces Pombe." Molecular Genetics and Genomics : MGG, vol. 289, no. 4, 2014, pp. 685-93.
Shimasaki T, Ohtsuka H, Naito C, et al. Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe. Mol Genet Genomics. 2014;289(4):685-93.
Shimasaki, T., Ohtsuka, H., Naito, C., Murakami, H., & Aiba, H. (2014). Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe. Molecular Genetics and Genomics : MGG, 289(4), 685-93. https://doi.org/10.1007/s00438-014-0845-1
Shimasaki T, et al. Ecl1 Is Activated By the Transcription Factor Atf1 in Response to H2O2 Stress in Schizosaccharomyces Pombe. Mol Genet Genomics. 2014;289(4):685-93. PubMed PMID: 24696293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ecl1 is activated by the transcription factor Atf1 in response to H2O2 stress in Schizosaccharomyces pombe. AU - Shimasaki,Takafumi, AU - Ohtsuka,Hokuto, AU - Naito,Chikako, AU - Murakami,Hiroshi, AU - Aiba,Hirofumi, Y1 - 2014/04/03/ PY - 2013/08/09/received PY - 2014/03/21/accepted PY - 2014/4/4/entrez PY - 2014/4/4/pubmed PY - 2014/9/25/medline SP - 685 EP - 93 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 289 IS - 4 N2 - The Ecl1 family genes extend the lifespan of fission yeast when overexpressed. They also cause resistance against H(2)O(2) stress. In this study, we found that the bZip transcription factor Atf1 is a direct activator of the induction of extender of chronological lifespan (ecl1 (+)) by H(2)O(2) stress. Based on ChIP analysis, we identified that Atf1 binds to the upstream DNA region of ecl1(+). Previously, we reported that overexpression of ecl1(+) increased the expression of the catalase-encoding ctt1(+). This ecl1(+)-dependent increase of ctt1(+) expression occurred in ∆atf1 mutant. On the other hand, the activation of ctt1 (+) caused by the ∆pyp1 mutation, which enhances Sty1-Atf1 activity, could occur in ∆ecl1 mutant. Based on these results, we propose that Atf1 can regulate ctt1(+) in both an Ecl1-dependent and an Ecl1-independent manner. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/24696293/Ecl1_is_activated_by_the_transcription_factor_Atf1_in_response_to_H2O2_stress_in_Schizosaccharomyces_pombe_ L2 - https://doi.org/10.1007/s00438-014-0845-1 DB - PRIME DP - Unbound Medicine ER -