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Giemsa staining for cysts and trophozoites of Pneumocystis carinii.
J Clin Pathol. 1989 Apr; 42(4):432-4.JC

Abstract

Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining with Giemsa allows cysts to be visualised, thus enabling a single stain to be used to show all the stages of BAL or sputum, which is particularly useful, considering the increase in the prevalence of P carinii pneumonia in conjunction with the spread of AIDS.

Authors+Show Affiliations

Department of Infectious Diseases and Microbiology, Westmead Hospital, Australia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2469702

Citation

Walker, J, et al. "Giemsa Staining for Cysts and Trophozoites of Pneumocystis Carinii." Journal of Clinical Pathology, vol. 42, no. 4, 1989, pp. 432-4.
Walker J, Conner G, Ho J, et al. Giemsa staining for cysts and trophozoites of Pneumocystis carinii. J Clin Pathol. 1989;42(4):432-4.
Walker, J., Conner, G., Ho, J., Hunt, C., & Pickering, L. (1989). Giemsa staining for cysts and trophozoites of Pneumocystis carinii. Journal of Clinical Pathology, 42(4), 432-4.
Walker J, et al. Giemsa Staining for Cysts and Trophozoites of Pneumocystis Carinii. J Clin Pathol. 1989;42(4):432-4. PubMed PMID: 2469702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Giemsa staining for cysts and trophozoites of Pneumocystis carinii. AU - Walker,J, AU - Conner,G, AU - Ho,J, AU - Hunt,C, AU - Pickering,L, PY - 1989/4/1/pubmed PY - 1989/4/1/medline PY - 1989/4/1/entrez SP - 432 EP - 4 JF - Journal of clinical pathology JO - J Clin Pathol VL - 42 IS - 4 N2 - Although Giemsa staining has been routinely used for the detection of trophozoites and intracystic bodies in smears of bronchoalveolar lavage fluid (BAL) from patients with Pneumocystis carinii pneumonia, it does not normally stain the cyst wall. For detection of the cysts other stains such as toluidine Blue 'O' and methenamine silver must be used as well. Sulphation of smears before staining with Giemsa allows cysts to be visualised, thus enabling a single stain to be used to show all the stages of BAL or sputum, which is particularly useful, considering the increase in the prevalence of P carinii pneumonia in conjunction with the spread of AIDS. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/2469702/Giemsa_staining_for_cysts_and_trophozoites_of_Pneumocystis_carinii_ L2 - https://jcp.bmj.com/lookup/pmidlookup?view=long&pmid=2469702 DB - PRIME DP - Unbound Medicine ER -