Tags

Type your tag names separated by a space and hit enter

A bioplex analysis of cytokines and chemokines in first trimester maternal plasma to screen for predictors of miscarriage.
PLoS One. 2014; 9(4):e93320.Plos

Abstract

BACKGROUND

We have previously shown in two independent cohorts that circulating first trimester Macrophage Inhibitory Cytokine-1 (MIC-1) levels are lower in women in early pregnancy who are destined to miscarriage. While promising, the diagnostic performance of measuring MIC-1 alone was not sufficient for it to be a useful predictive test for miscarriage. Besides MIC-1, there are other cytokines, as well as chemokines, involved in facilitating early pregnancy. We reasoned that screening these factors in maternal plasma could uncover other predictive markers of miscarriage.

METHODS

This was a nested case control study, of 78 women from a prospective study of 462 attending the Early Pregnancy Assessment Unit in the first trimester (EPAU) with a threatened miscarriage; 34 of these subsequently miscarried (cases) and 44 went on to have a normal delivery (controls) Cytokines IL-1β, IL-6 and IL-10, and the chemokines, CXCL8, CCL2, CCL5, CCL7 and CX3CL1 were measured in plasma from our cohort.

RESULTS

The cytokines IL-1β, IL-6, IL-10 and the chemokine CXCL8 were not detectable in first trimester plasma. The chemokines CCL2, CCL5, CCL7 and CX3CL1 were detectable in all samples but levels did not vary across 5-12 weeks of gestation among controls. Plasma levels of these chemokines were no different in the miscarriage cohort compared to controls.

CONCLUSION

The chemokines CCL2, CCL5, CCL7 and CX3CL1 were detectable in plasma during the first trimester while IL-1β, IL-6, IL-10 and CXCL8 were not. However, none of the cytokines and chemokines screened were different in maternal plasma in cases or controls. These therefore do not appear to have potential for application as predictive biomarkers of miscarriage.

Authors+Show Affiliations

Translational Obstetrics Group, The Department of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria, Australia.Endocannabinoid Research Group, Reproductive Sciences Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, United Kingdom.Translational Obstetrics Group, The Department of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria, Australia.Endocannabinoid Research Group, Reproductive Sciences Section, Department of Cancer Studies and Molecular Medicine, University of Leicester, Leicester, United Kingdom.Translational Obstetrics Group, The Department of Obstetrics and Gynaecology, Mercy Hospital for Women, University of Melbourne, Heidelberg, Victoria, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24699265

Citation

Hannan, Natalie J., et al. "A Bioplex Analysis of Cytokines and Chemokines in First Trimester Maternal Plasma to Screen for Predictors of Miscarriage." PloS One, vol. 9, no. 4, 2014, pp. e93320.
Hannan NJ, Bambang K, Kaitu'u-Lino TJ, et al. A bioplex analysis of cytokines and chemokines in first trimester maternal plasma to screen for predictors of miscarriage. PLoS ONE. 2014;9(4):e93320.
Hannan, N. J., Bambang, K., Kaitu'u-Lino, T. J., Konje, J. C., & Tong, S. (2014). A bioplex analysis of cytokines and chemokines in first trimester maternal plasma to screen for predictors of miscarriage. PloS One, 9(4), e93320. https://doi.org/10.1371/journal.pone.0093320
Hannan NJ, et al. A Bioplex Analysis of Cytokines and Chemokines in First Trimester Maternal Plasma to Screen for Predictors of Miscarriage. PLoS ONE. 2014;9(4):e93320. PubMed PMID: 24699265.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A bioplex analysis of cytokines and chemokines in first trimester maternal plasma to screen for predictors of miscarriage. AU - Hannan,Natalie J, AU - Bambang,Katerina, AU - Kaitu'u-Lino,Tu'uhevaha J, AU - Konje,Justin C, AU - Tong,Stephen, Y1 - 2014/04/03/ PY - 2013/11/18/received PY - 2014/03/04/accepted PY - 2014/4/5/entrez PY - 2014/4/5/pubmed PY - 2015/1/21/medline SP - e93320 EP - e93320 JF - PloS one JO - PLoS ONE VL - 9 IS - 4 N2 - BACKGROUND: We have previously shown in two independent cohorts that circulating first trimester Macrophage Inhibitory Cytokine-1 (MIC-1) levels are lower in women in early pregnancy who are destined to miscarriage. While promising, the diagnostic performance of measuring MIC-1 alone was not sufficient for it to be a useful predictive test for miscarriage. Besides MIC-1, there are other cytokines, as well as chemokines, involved in facilitating early pregnancy. We reasoned that screening these factors in maternal plasma could uncover other predictive markers of miscarriage. METHODS: This was a nested case control study, of 78 women from a prospective study of 462 attending the Early Pregnancy Assessment Unit in the first trimester (EPAU) with a threatened miscarriage; 34 of these subsequently miscarried (cases) and 44 went on to have a normal delivery (controls) Cytokines IL-1β, IL-6 and IL-10, and the chemokines, CXCL8, CCL2, CCL5, CCL7 and CX3CL1 were measured in plasma from our cohort. RESULTS: The cytokines IL-1β, IL-6, IL-10 and the chemokine CXCL8 were not detectable in first trimester plasma. The chemokines CCL2, CCL5, CCL7 and CX3CL1 were detectable in all samples but levels did not vary across 5-12 weeks of gestation among controls. Plasma levels of these chemokines were no different in the miscarriage cohort compared to controls. CONCLUSION: The chemokines CCL2, CCL5, CCL7 and CX3CL1 were detectable in plasma during the first trimester while IL-1β, IL-6, IL-10 and CXCL8 were not. However, none of the cytokines and chemokines screened were different in maternal plasma in cases or controls. These therefore do not appear to have potential for application as predictive biomarkers of miscarriage. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24699265/A_bioplex_analysis_of_cytokines_and_chemokines_in_first_trimester_maternal_plasma_to_screen_for_predictors_of_miscarriage_ L2 - http://dx.plos.org/10.1371/journal.pone.0093320 DB - PRIME DP - Unbound Medicine ER -