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An off-on-off electrochemiluminescence approach for ultrasensitive detection of thrombin.
Biosens Bioelectron. 2014 Sep 15; 59:58-63.BB

Abstract

This work demonstrates an aptasensor for ultrasensitive electrochemiluminescence (ECL) detection of thrombin based on an "off-on-off" approach. The system is composed of an Eu(3+)-doped CdS nanocrystals (CdS:Eu NCs) film on glassy carbon electrode (GCE) as ECL emitter. Then gold nanoparticles (AuNPs) labeled hairpin-DNA probe (ssDNA1) containing thrombin-binding aptamer (TBA) sequence was linked on the NCs film, which led to ECL quenching (off) as a result of Förster-resonance energy transfer (FRET) between the CdS:Eu NC film and the proximal AuNPs. Upon the occurrence of hybridization with its complementary DNA (ssDNA2), an ECL enhancement (on) occurred owing to the interactions of the excited CdS:Eu NCs with ECL-induced surface plasmon resonance (SPR) in AuNPs at large separation. Thrombin could induce ssDNA1 forming a G-quadruplex and cause the AuNPs to be close to CdS:Eu NCs film again, which resulted in an enhanced ECL quenching (off). This "off-on-off" system showed a maximum 7.4-fold change of ECL intensity due to the configuration transformation of ssDNA1 and provides great sensitivity for detection of thrombin in a wide detection range from 50 aM to 1 pM.

Authors+Show Affiliations

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.Academy of Fundamental and Interdisciplinary Sciences, Harbin Institute of Technology, Harbin 150080, China.State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. Electronic address: xujj@nju.edu.cn.State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China.

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24699694

Citation

Deng, Li, et al. "An Off-on-off Electrochemiluminescence Approach for Ultrasensitive Detection of Thrombin." Biosensors & Bioelectronics, vol. 59, 2014, pp. 58-63.
Deng L, Du Y, Xu JJ, et al. An off-on-off electrochemiluminescence approach for ultrasensitive detection of thrombin. Biosens Bioelectron. 2014;59:58-63.
Deng, L., Du, Y., Xu, J. J., & Chen, H. Y. (2014). An off-on-off electrochemiluminescence approach for ultrasensitive detection of thrombin. Biosensors & Bioelectronics, 59, 58-63. https://doi.org/10.1016/j.bios.2014.03.012
Deng L, et al. An Off-on-off Electrochemiluminescence Approach for Ultrasensitive Detection of Thrombin. Biosens Bioelectron. 2014 Sep 15;59:58-63. PubMed PMID: 24699694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An off-on-off electrochemiluminescence approach for ultrasensitive detection of thrombin. AU - Deng,Li, AU - Du,Ying, AU - Xu,Jing-Juan, AU - Chen,Hong-Yuan, Y1 - 2014/03/15/ PY - 2013/11/26/received PY - 2014/02/18/revised PY - 2014/03/06/accepted PY - 2014/4/5/entrez PY - 2014/4/5/pubmed PY - 2015/1/13/medline KW - Electrochemiluminescence KW - Eu(3+)-doped CdS nanocrystals KW - Gold nanoparticles KW - Resonance energy transfer KW - Thrombin binding aptamer SP - 58 EP - 63 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 59 N2 - This work demonstrates an aptasensor for ultrasensitive electrochemiluminescence (ECL) detection of thrombin based on an "off-on-off" approach. The system is composed of an Eu(3+)-doped CdS nanocrystals (CdS:Eu NCs) film on glassy carbon electrode (GCE) as ECL emitter. Then gold nanoparticles (AuNPs) labeled hairpin-DNA probe (ssDNA1) containing thrombin-binding aptamer (TBA) sequence was linked on the NCs film, which led to ECL quenching (off) as a result of Förster-resonance energy transfer (FRET) between the CdS:Eu NC film and the proximal AuNPs. Upon the occurrence of hybridization with its complementary DNA (ssDNA2), an ECL enhancement (on) occurred owing to the interactions of the excited CdS:Eu NCs with ECL-induced surface plasmon resonance (SPR) in AuNPs at large separation. Thrombin could induce ssDNA1 forming a G-quadruplex and cause the AuNPs to be close to CdS:Eu NCs film again, which resulted in an enhanced ECL quenching (off). This "off-on-off" system showed a maximum 7.4-fold change of ECL intensity due to the configuration transformation of ssDNA1 and provides great sensitivity for detection of thrombin in a wide detection range from 50 aM to 1 pM. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/24699694/An_off_on_off_electrochemiluminescence_approach_for_ultrasensitive_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(14)00193-6 DB - PRIME DP - Unbound Medicine ER -