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Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust.
Mol Genet Genomics. 2014 Oct; 289(5):735-43.MG

Abstract

We characterized a wheat-Psathyrostachys huashanica derived line 3-6-4-1 based on genomic in situ hybridization (GISH), molecular marker analysis, and agronomic trait evaluations. The GISH investigations showed that the 3-6-4-1 contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The homoeologous relationships of the introduced P. huashanica chromosomes were determined using EST-STS multiple loci markers from seven wheat homoeologous groups in the parents and the addition line. Twelve EST-STS markers located on the homoeologous group 2 chromosomes of wheat amplified polymorphic bands in 3-6-4-1, which were unique to P. huashanica. An introduced Ns chromosome pair that belonged to homoeologous group 2 was identified using chromosome-specific markers. Inoculation with isolates of the stripe rust pathotypes, CYR31, CYR32, and SY11-14, and mixed races (CYR31, CYR32, and SY11-14) in the seeding and adult stage, respectively, showed that 3-6-4-1 was generally resistant to stripe rust, which was probably attributable to its P. huashanica parent. We also compared a complete set of wheat-P. huashanica disomic addition lines (1Ns-7Ns, 2n = 44 = 22II) to assess their agronomic traits and morphological characteristics, which showed that 3-6-4-1 had improved spike traits compared with its parents. The P. huashanica 2Ns chromosome-specific molecular markers in 3-6-4-1 could be useful for marker-assisted selection in breeding programs to combat stripe rust. This line can be used as a donor source to introduce novel excellent genes from P. huashanica into wheat to widen its genetic diversity, thereby providing new germplasms for wheat breeding.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Du W
Shaanxi Key Laboratory of Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China, dwl2105@163.com.
Wang J
No affiliation info available
Wang L
No affiliation info available
Wu J
No affiliation info available
Zhao J
No affiliation info available
Liu S
No affiliation info available
Yang Q
No affiliation info available
Chen X
No affiliation info available

MeSH

Base SequenceBasidiomycotaChromosomes, PlantDiploidyDisease ResistanceExpressed Sequence TagsGenes, PlantGenetic LinkageHybridization, GeneticPlant DiseasesSeedlingsTriticum

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24700077

Citation

Du, Wanli, et al. "Molecular Characterization of a wheat-Psathyrostachys Huashanica Keng 2Ns Disomic Addition Line With Resistance to Stripe Rust." Molecular Genetics and Genomics : MGG, vol. 289, no. 5, 2014, pp. 735-43.
Du W, Wang J, Wang L, et al. Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust. Mol Genet Genomics. 2014;289(5):735-43.
Du, W., Wang, J., Wang, L., Wu, J., Zhao, J., Liu, S., Yang, Q., & Chen, X. (2014). Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust. Molecular Genetics and Genomics : MGG, 289(5), 735-43. https://doi.org/10.1007/s00438-014-0844-2
Du W, et al. Molecular Characterization of a wheat-Psathyrostachys Huashanica Keng 2Ns Disomic Addition Line With Resistance to Stripe Rust. Mol Genet Genomics. 2014;289(5):735-43. PubMed PMID: 24700077.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular characterization of a wheat-Psathyrostachys huashanica Keng 2Ns disomic addition line with resistance to stripe rust. AU - Du,Wanli, AU - Wang,Jing, AU - Wang,Liangming, AU - Wu,Jun, AU - Zhao,Jixin, AU - Liu,Shuhui, AU - Yang,Qunhui, AU - Chen,Xinhong, Y1 - 2014/04/04/ PY - 2013/12/29/received PY - 2014/03/21/accepted PY - 2014/4/5/entrez PY - 2014/4/5/pubmed PY - 2014/11/18/medline SP - 735 EP - 43 JF - Molecular genetics and genomics : MGG JO - Mol Genet Genomics VL - 289 IS - 5 N2 - We characterized a wheat-Psathyrostachys huashanica derived line 3-6-4-1 based on genomic in situ hybridization (GISH), molecular marker analysis, and agronomic trait evaluations. The GISH investigations showed that the 3-6-4-1 contained 42 wheat chromosomes and a pair of P. huashanica chromosomes. The homoeologous relationships of the introduced P. huashanica chromosomes were determined using EST-STS multiple loci markers from seven wheat homoeologous groups in the parents and the addition line. Twelve EST-STS markers located on the homoeologous group 2 chromosomes of wheat amplified polymorphic bands in 3-6-4-1, which were unique to P. huashanica. An introduced Ns chromosome pair that belonged to homoeologous group 2 was identified using chromosome-specific markers. Inoculation with isolates of the stripe rust pathotypes, CYR31, CYR32, and SY11-14, and mixed races (CYR31, CYR32, and SY11-14) in the seeding and adult stage, respectively, showed that 3-6-4-1 was generally resistant to stripe rust, which was probably attributable to its P. huashanica parent. We also compared a complete set of wheat-P. huashanica disomic addition lines (1Ns-7Ns, 2n = 44 = 22II) to assess their agronomic traits and morphological characteristics, which showed that 3-6-4-1 had improved spike traits compared with its parents. The P. huashanica 2Ns chromosome-specific molecular markers in 3-6-4-1 could be useful for marker-assisted selection in breeding programs to combat stripe rust. This line can be used as a donor source to introduce novel excellent genes from P. huashanica into wheat to widen its genetic diversity, thereby providing new germplasms for wheat breeding. SN - 1617-4623 UR - https://www.unboundmedicine.com/medline/citation/24700077/Molecular_characterization_of_a_wheat_Psathyrostachys_huashanica_Keng_2Ns_disomic_addition_line_with_resistance_to_stripe_rust_ DB - PRIME DP - Unbound Medicine ER -