Tags

Type your tag names separated by a space and hit enter

Genotypic characterization of Escherichia coli O157:H7 strains that cause diarrhea and hemolytic uremic syndrome in Neuquén, Argentina.
Int J Med Microbiol. 2014 May; 304(3-4):499-504.IJ

Abstract

Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens associated with cases of diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). E. coli O157:H7 is the dominant serotype in Argentina and also in Neuquén Province, in which HUS incidence is above the national average, with a maximum of 28.6 cases per 100,000 children less than 5 years old reported in 1998. The aim of this study was to characterize a collection of 70 STEC O157 strains isolated from patients with diarrhea and HUS treated in the province of Neuquén, Argentina, between 1998 and 2011. All strains harbored eae, ehxA, rfbO157, and fliCH7 genes, and stx2a/stx2c (78.7%) was the predominant genotype. A total of 64 (91.4%) STEC O157 strains belonged to the hypervirulent clade 8 tested using both 4 and 32 SNP typing schemes. The strains showed the highest values reported in the literature for 6 of the 7 virulence determinants described in the TW14359 O157 strain associated with the raw spinach outbreak in the U.S. in 2006. Clade 8 strains were strongly associated with two of them: ECSP_3286, factor encoding an outer membrane protein that facilitates the transport of the heme complex (P=0.001), and in particular extracellular factor ECSP_2870/2872, coding proteins related to adaptation to plant hosts (P=0.000004). The q933 allele, which has been related to high toxin production, was present in 97.1% of the strains studied for the anti-terminator Q gene. In summary, this study describes, for the first time in Argentina, the almost exclusive circulation of strains belonging to the hypervirulent clade 8, and also the presence of putative virulence factors in higher frequencies than those reported worldwide. These data may help to understand the causes of the particular epidemiological situation related to HUS in Neuquén Province.

Authors+Show Affiliations

Laboratorio Central, Subsecretaría de Salud de Neuquén, Gregorio Martínez 65, 8300 Neuquén, Argentina. Electronic address: luispianciola@yahoo.com.ar.Servicio Fisiopatogenia, INEI-ANLIS "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.Laboratorio Central, Subsecretaría de Salud de Neuquén, Gregorio Martínez 65, 8300 Neuquén, Argentina.Servicio Fisiopatogenia, INEI-ANLIS "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.Laboratorio de Microbiología, Hospital "Dr. Horacio Heller", Godoy y Lighuén, 8300 Neuquén, Argentina.Laboratorio Central, Subsecretaría de Salud de Neuquén, Gregorio Martínez 65, 8300 Neuquén, Argentina.Servicio Fisiopatogenia, INEI-ANLIS "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.Laboratorio Central, Subsecretaría de Salud de Neuquén, Gregorio Martínez 65, 8300 Neuquén, Argentina.Laboratorio Central, Subsecretaría de Salud de Neuquén, Gregorio Martínez 65, 8300 Neuquén, Argentina.Servicio Fisiopatogenia, INEI-ANLIS "Dr. Carlos G. Malbrán", Av. Vélez Sarsfield 563, 1281 Buenos Aires, Argentina.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24702854

Citation

Pianciola, Luis, et al. "Genotypic Characterization of Escherichia Coli O157:H7 Strains That Cause Diarrhea and Hemolytic Uremic Syndrome in Neuquén, Argentina." International Journal of Medical Microbiology : IJMM, vol. 304, no. 3-4, 2014, pp. 499-504.
Pianciola L, Chinen I, Mazzeo M, et al. Genotypic characterization of Escherichia coli O157:H7 strains that cause diarrhea and hemolytic uremic syndrome in Neuquén, Argentina. Int J Med Microbiol. 2014;304(3-4):499-504.
Pianciola, L., Chinen, I., Mazzeo, M., Miliwebsky, E., González, G., Müller, C., Carbonari, C., Navello, M., Zitta, E., & Rivas, M. (2014). Genotypic characterization of Escherichia coli O157:H7 strains that cause diarrhea and hemolytic uremic syndrome in Neuquén, Argentina. International Journal of Medical Microbiology : IJMM, 304(3-4), 499-504. https://doi.org/10.1016/j.ijmm.2014.02.011
Pianciola L, et al. Genotypic Characterization of Escherichia Coli O157:H7 Strains That Cause Diarrhea and Hemolytic Uremic Syndrome in Neuquén, Argentina. Int J Med Microbiol. 2014;304(3-4):499-504. PubMed PMID: 24702854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotypic characterization of Escherichia coli O157:H7 strains that cause diarrhea and hemolytic uremic syndrome in Neuquén, Argentina. AU - Pianciola,Luis, AU - Chinen,Isabel, AU - Mazzeo,Melina, AU - Miliwebsky,Elizabeth, AU - González,Gladys, AU - Müller,Constanza, AU - Carbonari,Carolina, AU - Navello,Mariano, AU - Zitta,Eugenia, AU - Rivas,Marta, Y1 - 2014/03/18/ PY - 2014/01/02/received PY - 2014/02/17/revised PY - 2014/02/22/accepted PY - 2014/4/8/entrez PY - 2014/4/8/pubmed PY - 2014/12/15/medline KW - Clades KW - E. coli KW - Hypervirulent KW - O157 KW - stx2 KW - stx2c SP - 499 EP - 504 JF - International journal of medical microbiology : IJMM JO - Int J Med Microbiol VL - 304 IS - 3-4 N2 - Shiga toxin-producing Escherichia coli (STEC) are important food-borne pathogens associated with cases of diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). E. coli O157:H7 is the dominant serotype in Argentina and also in Neuquén Province, in which HUS incidence is above the national average, with a maximum of 28.6 cases per 100,000 children less than 5 years old reported in 1998. The aim of this study was to characterize a collection of 70 STEC O157 strains isolated from patients with diarrhea and HUS treated in the province of Neuquén, Argentina, between 1998 and 2011. All strains harbored eae, ehxA, rfbO157, and fliCH7 genes, and stx2a/stx2c (78.7%) was the predominant genotype. A total of 64 (91.4%) STEC O157 strains belonged to the hypervirulent clade 8 tested using both 4 and 32 SNP typing schemes. The strains showed the highest values reported in the literature for 6 of the 7 virulence determinants described in the TW14359 O157 strain associated with the raw spinach outbreak in the U.S. in 2006. Clade 8 strains were strongly associated with two of them: ECSP_3286, factor encoding an outer membrane protein that facilitates the transport of the heme complex (P=0.001), and in particular extracellular factor ECSP_2870/2872, coding proteins related to adaptation to plant hosts (P=0.000004). The q933 allele, which has been related to high toxin production, was present in 97.1% of the strains studied for the anti-terminator Q gene. In summary, this study describes, for the first time in Argentina, the almost exclusive circulation of strains belonging to the hypervirulent clade 8, and also the presence of putative virulence factors in higher frequencies than those reported worldwide. These data may help to understand the causes of the particular epidemiological situation related to HUS in Neuquén Province. SN - 1618-0607 UR - https://www.unboundmedicine.com/medline/citation/24702854/Genotypic_characterization_of_Escherichia_coli_O157:H7_strains_that_cause_diarrhea_and_hemolytic_uremic_syndrome_in_Neuquén_Argentina_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1438-4221(14)00021-6 DB - PRIME DP - Unbound Medicine ER -