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Resistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary males.
Exp Physiol. 2014 Jun; 99(6):894-908.EP

Abstract

Recent in vitro and in vivo experimental observations suggest that improvements in insulin sensitivity following endurance training are mechanistically linked to increases in muscle oxidative capacity, intramuscular triglyceride (IMTG) utilization during endurance exercise and increases in the content of the lipid droplet-associated perilipin 2 (PLIN2) and perilipin 5 (PLIN5). This study investigated the hypothesis that similar adaptations may also underlie the resistance training (RT)-induced improvements in insulin sensitivity. Thirteen sedentary men (20 ± 1 years old; body mass index 24.8 ± 0.8 kg m(-2)) performed 6 weeks of whole-body RT (three times per week), and changes in peak O2 uptake (in millilitres per minute per kilogram) and insulin sensitivity were assessed. Muscle biopsies (n = 8) were obtained before and after 60 min steady-state cycling at ~65% peak O2 uptake. Immunofluorescence microscopy was used to assess changes in oxidative capacity (measured as cytochrome c oxidase protein content), IMTG and PLIN2 and PLIN5 protein content. Resistance training increased peak O2 uptake (by 8 ± 3%), COX protein content (by 46 ± 13 and 61 ± 13% in type I and II fibres, respectively) and the Matsuda insulin sensitivity index (by 47 ± 6%; all P < 0.05). In type I fibres, IMTG (by 52 ± 11%; P < 0.05) and PLIN2 content (by 107 ± 19%; P < 0.05) were increased and PLIN5 content tended to increase (by 54 ± 22%; P = 0.054) post-training. In type II fibres, PLIN2 content increased (by 57 ± 20%; P < 0.05) and IMTG (by 46 ± 17%; P = 0.1) and PLIN5 content (by 44 ± 24%; P = 0.054) tended to increase post-training. Breakdown of IMTG during moderate-intensity exercise was greater in both type I and type II fibres (by 43 ± 5 and 37 ± 5%, respectively; P < 0.05) post-RT. The results confirm the hypothesis that RT enhances muscle oxidative capacity and increases IMTG breakdown and the content of PLIN2 and PLIN5 in both type I and type II fibres during endurance-type exercise.

Authors+Show Affiliations

Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, Liverpool, UK.Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, Liverpool, UK.Health and Exercise Sciences Research Group, University of Stirling, Stirling, UK.Health and Exercise Sciences Research Group, University of Stirling, Stirling, UK.School of Clinical and Experimental Medicine, Cardiovascular and Respiratory Sciences, University of Birmingham, Birmingham, UK.School of Clinical and Experimental Medicine, Cardiovascular and Respiratory Sciences, University of Birmingham, Birmingham, UK.Research Institute for Sport & Exercise Sciences, Liverpool John Moores University, Liverpool, UK a.j.wagenmakers@ljmu.ac.uk.Institute of Sport, Exercise & Active Living, Victoria University, Melbourne, Victoria, Australia.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24706192

Citation

Shepherd, S O., et al. "Resistance Training Increases Skeletal Muscle Oxidative Capacity and Net Intramuscular Triglyceride Breakdown in Type I and II Fibres of Sedentary Males." Experimental Physiology, vol. 99, no. 6, 2014, pp. 894-908.
Shepherd SO, Cocks M, Tipton KD, et al. Resistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary males. Exp Physiol. 2014;99(6):894-908.
Shepherd, S. O., Cocks, M., Tipton, K. D., Witard, O. C., Ranasinghe, A. M., Barker, T. A., Wagenmakers, A. J., & Shaw, C. S. (2014). Resistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary males. Experimental Physiology, 99(6), 894-908. https://doi.org/10.1113/expphysiol.2014.078014
Shepherd SO, et al. Resistance Training Increases Skeletal Muscle Oxidative Capacity and Net Intramuscular Triglyceride Breakdown in Type I and II Fibres of Sedentary Males. Exp Physiol. 2014;99(6):894-908. PubMed PMID: 24706192.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Resistance training increases skeletal muscle oxidative capacity and net intramuscular triglyceride breakdown in type I and II fibres of sedentary males. AU - Shepherd,S O, AU - Cocks,M, AU - Tipton,K D, AU - Witard,O C, AU - Ranasinghe,A M, AU - Barker,T A, AU - Wagenmakers,A J M, AU - Shaw,C S, Y1 - 2014/04/04/ PY - 2014/4/8/entrez PY - 2014/4/8/pubmed PY - 2015/1/27/medline SP - 894 EP - 908 JF - Experimental physiology JO - Exp Physiol VL - 99 IS - 6 N2 - Recent in vitro and in vivo experimental observations suggest that improvements in insulin sensitivity following endurance training are mechanistically linked to increases in muscle oxidative capacity, intramuscular triglyceride (IMTG) utilization during endurance exercise and increases in the content of the lipid droplet-associated perilipin 2 (PLIN2) and perilipin 5 (PLIN5). This study investigated the hypothesis that similar adaptations may also underlie the resistance training (RT)-induced improvements in insulin sensitivity. Thirteen sedentary men (20 ± 1 years old; body mass index 24.8 ± 0.8 kg m(-2)) performed 6 weeks of whole-body RT (three times per week), and changes in peak O2 uptake (in millilitres per minute per kilogram) and insulin sensitivity were assessed. Muscle biopsies (n = 8) were obtained before and after 60 min steady-state cycling at ~65% peak O2 uptake. Immunofluorescence microscopy was used to assess changes in oxidative capacity (measured as cytochrome c oxidase protein content), IMTG and PLIN2 and PLIN5 protein content. Resistance training increased peak O2 uptake (by 8 ± 3%), COX protein content (by 46 ± 13 and 61 ± 13% in type I and II fibres, respectively) and the Matsuda insulin sensitivity index (by 47 ± 6%; all P < 0.05). In type I fibres, IMTG (by 52 ± 11%; P < 0.05) and PLIN2 content (by 107 ± 19%; P < 0.05) were increased and PLIN5 content tended to increase (by 54 ± 22%; P = 0.054) post-training. In type II fibres, PLIN2 content increased (by 57 ± 20%; P < 0.05) and IMTG (by 46 ± 17%; P = 0.1) and PLIN5 content (by 44 ± 24%; P = 0.054) tended to increase post-training. Breakdown of IMTG during moderate-intensity exercise was greater in both type I and type II fibres (by 43 ± 5 and 37 ± 5%, respectively; P < 0.05) post-RT. The results confirm the hypothesis that RT enhances muscle oxidative capacity and increases IMTG breakdown and the content of PLIN2 and PLIN5 in both type I and type II fibres during endurance-type exercise. SN - 1469-445X UR - https://www.unboundmedicine.com/medline/citation/24706192/Resistance_training_increases_skeletal_muscle_oxidative_capacity_and_net_intramuscular_triglyceride_breakdown_in_type_I_and_II_fibres_of_sedentary_males_ L2 - https://doi.org/10.1113/expphysiol.2014.078014 DB - PRIME DP - Unbound Medicine ER -