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Effect of Ajuga bracteosa on systemic T-cell immunity in Balb/C mice: dual Th1/Th2 immunostimulatory effects.
Am J Chin Med. 2014; 42(2):375-92.AJ

Abstract

Ajuga bracteosa (AB) has been widely used in folk medicine in Asian countries against gout, hepatitis, pneumonia, rheumatism, and various neuro inflammatory disorders. The aim of this study was to investigate the possible immunoregulatory effects of the ethanolic extract of Ajuga bracteosa (ABEE) on systemic Th1/Th2 immunity in SRBC immunized Balb/C mice. Animals were orally administered with graded doses of ABEE from 6.25 mg/kg to 100 mg/kg. Post sub-cutaneous immunization with SRBCs and circulating antibody titers, DTH responses and splenocyte proliferation was monitored as markers of Th2 and Th1 responses. Cyclophosphamide and levamisole were used as controls. Lymphocyte immunophenotying (CD4/CD8 cell counts) and intracellular Th1/Th2 cytokine concentrations were determined using flow cytometry. Treatment with ABEE demonstrated significant biphasic immunostimulation of effector T-helper immunity. ABEE at 50 mg/kg dose resulted in maximal increase in antibody titers, DTH responses and CD4+/CD8+ T-cell percentages indicating maximal activation and proliferation of T and B lymphocytes at this dose. ABEE, at the same dose, also showed maximal up regulation of LPS and CON A stimulated splenocyte proliferation and also maximal up-regulation of both Th1 (IL-2, IFN-γ) and Th2 (IL-4) cytokines which suggest its mixed Th1/Th2 immunostimulatory activity. Comparatively at higher doses (100 mg/kg), significant down regulation of all these effector T-helper (Th) immune responses was observed. The study therefore suggests mixed biphasic immunostimulatory Th1/Th2 activity of ABEE that could support its immunoadjuvant potential.

Authors+Show Affiliations

Department of Biochemistry, University of Kashmir, Srinagar, India-190008, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24707869

Citation

Nisar, Albeena, et al. "Effect of Ajuga Bracteosa On Systemic T-cell Immunity in Balb/C Mice: Dual Th1/Th2 Immunostimulatory Effects." The American Journal of Chinese Medicine, vol. 42, no. 2, 2014, pp. 375-92.
Nisar A, Akhtar N, Hassan A, et al. Effect of Ajuga bracteosa on systemic T-cell immunity in Balb/C mice: dual Th1/Th2 immunostimulatory effects. Am J Chin Med. 2014;42(2):375-92.
Nisar, A., Akhtar, N., Hassan, A., Banday, T., Wani, B., & Zargar, M. A. (2014). Effect of Ajuga bracteosa on systemic T-cell immunity in Balb/C mice: dual Th1/Th2 immunostimulatory effects. The American Journal of Chinese Medicine, 42(2), 375-92. https://doi.org/10.1142/S0192415X14500256
Nisar A, et al. Effect of Ajuga Bracteosa On Systemic T-cell Immunity in Balb/C Mice: Dual Th1/Th2 Immunostimulatory Effects. Am J Chin Med. 2014;42(2):375-92. PubMed PMID: 24707869.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Ajuga bracteosa on systemic T-cell immunity in Balb/C mice: dual Th1/Th2 immunostimulatory effects. AU - Nisar,Albeena, AU - Akhtar,Nayeema, AU - Hassan,Asma, AU - Banday,Tabish, AU - Wani,Bilal, AU - Zargar,Mohammed Afzal, PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2014/10/29/medline SP - 375 EP - 92 JF - The American journal of Chinese medicine JO - Am J Chin Med VL - 42 IS - 2 N2 - Ajuga bracteosa (AB) has been widely used in folk medicine in Asian countries against gout, hepatitis, pneumonia, rheumatism, and various neuro inflammatory disorders. The aim of this study was to investigate the possible immunoregulatory effects of the ethanolic extract of Ajuga bracteosa (ABEE) on systemic Th1/Th2 immunity in SRBC immunized Balb/C mice. Animals were orally administered with graded doses of ABEE from 6.25 mg/kg to 100 mg/kg. Post sub-cutaneous immunization with SRBCs and circulating antibody titers, DTH responses and splenocyte proliferation was monitored as markers of Th2 and Th1 responses. Cyclophosphamide and levamisole were used as controls. Lymphocyte immunophenotying (CD4/CD8 cell counts) and intracellular Th1/Th2 cytokine concentrations were determined using flow cytometry. Treatment with ABEE demonstrated significant biphasic immunostimulation of effector T-helper immunity. ABEE at 50 mg/kg dose resulted in maximal increase in antibody titers, DTH responses and CD4+/CD8+ T-cell percentages indicating maximal activation and proliferation of T and B lymphocytes at this dose. ABEE, at the same dose, also showed maximal up regulation of LPS and CON A stimulated splenocyte proliferation and also maximal up-regulation of both Th1 (IL-2, IFN-γ) and Th2 (IL-4) cytokines which suggest its mixed Th1/Th2 immunostimulatory activity. Comparatively at higher doses (100 mg/kg), significant down regulation of all these effector T-helper (Th) immune responses was observed. The study therefore suggests mixed biphasic immunostimulatory Th1/Th2 activity of ABEE that could support its immunoadjuvant potential. SN - 1793-6853 UR - https://www.unboundmedicine.com/medline/citation/24707869/Effect_of_Ajuga_bracteosa_on_systemic_T_cell_immunity_in_Balb/C_mice:_dual_Th1/Th2_immunostimulatory_effects_ L2 - https://www.worldscientific.com/doi/10.1142/S0192415X14500256?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -