TY - JOUR T1 - Galactomannan with novel structure produced by the coral endophytic fungus Aspergillus ochraceus. AU - Guo,Shoudong, AU - Mao,Wenjun, AU - Yan,Mengxia, AU - Zhao,Chunqi, AU - Li,Na, AU - Shan,Jimiao, AU - Lin,Cong, AU - Liu,Xue, AU - Guo,Tao, AU - Guo,Tiantian, AU - Wang,Shuyao, Y1 - 2014/01/31/ PY - 2013/08/16/received PY - 2014/01/17/revised PY - 2014/01/22/accepted PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2015/10/16/medline KW - Aspergillus ochraceus KW - ES-CID MS/MS KW - Extracellular polysaccharide KW - Galacto-oligosaccharide KW - NMR SP - 325 EP - 33 JF - Carbohydrate polymers JO - Carbohydr Polym VL - 105 N2 - The homogeneous extracellular polysaccharide, AW1, was obtained from the fermented broth of the fungus Aspergillus ochraceus derived from coral Dichotella gemmacea. AW1 was a galactomannan with a molar ratio of mannose and galactose of 2.16:1.00 and a molecular weight of about 29.0kDa. The structure of AW1 was investigated by chemical and spectroscopic methods, including methylation analysis, one- and two-dimensional nuclear magnetic resonance (1D, 2D NMR) and electrospray mass spectrometry with collision-induced dissociation (ES-CID MS/MS) spectroscopic analyses. The results showed that the backbone of AW1 consisted of (1⟶2)-linked α-d-mannopyranose residues. The mannopyranose residues in the backbone were substituted at C-6 by the (1⟶)-linked α-d-mannopyranose units and (1⟶5)-linked β-d-galactofuranose oligosaccharides with different degrees of polymerization. The investigation demonstrated that AW1 was a novel galactomannan with different structural characteristics from other fungal galactomannans, and could be a potential resource of the (1⟶5)-linked β-d-galactofuranose oligosaccharides. SN - 1879-1344 UR - https://www.unboundmedicine.com/medline/citation/24708987/Galactomannan_with_novel_structure_produced_by_the_coral_endophytic_fungus_Aspergillus_ochraceus_ DB - PRIME DP - Unbound Medicine ER -