Tags

Type your tag names separated by a space and hit enter

Characteristics of antibiotic resistance and sequence type of Acinetobacter baumannii clinical isolates in Japan and the antibacterial activity of DS-8587.
J Infect Chemother. 2014 Apr; 20(4):256-61.JI

Abstract

DS-8587 is a novel broad-spectrum fluoroquinolone with extended antimicrobial activity against both Gram-positive and Gram-negative pathogens. In this study, we evaluated the antibacterial activity and mechanism of DS-8587 in 31 quinolone-resistant Acinetobacter baumannii clinical isolates. Efflux pump and qnr genes, mutations in quinolone resistance-determining regions of target enzymes, and sequence types determined by multilocus sequence typing were analyzed. Forty-two quinolone-susceptible clinical isolates were analyzed for comparison. For susceptibility testing, DS-8587 exhibited more effective antibacterial activity when compared with ciprofloxacin and levofloxacin. When combined with the efflux pump inhibitor 1-(1-napthylmethyl)-piperazine, the MIC of DS-8587 was less affected when compared with the MIC exhibited by combined ciprofloxacin and 1-(1-napthylmethyl)-piperazine. The efflux pump genes adeA/adeB/adeC and regulatory elements adeR/adeS were detected in 23 of 31 quinolone-resistant isolates. The qnrA/qnrB/qnrS genes were not detected in any A. baumannii isolates analyzed. Mutations in quinolone resistance-determining regions were observed in all 31 quinolone-resistant isolates. Multilocus sequence typing analyses revealed that 22 of 31 quinolone-resistant isolates belonged to ST-2, corresponding to international clonal lineage II. In conclusion, DS-8587 exhibits potent antibacterial activity against quinolone-resistant A. baumannii isolates that harbor mutations in quinolone resistance-determining regions. In the presence of the efflux pump inhibitor 1-(1-napthylmethyl)-piperazine, no significant changes were observed in the MIC for DS-8587. DS-8587 should be considered as a treatment option for A. baumannii including ST-2 strains that are predominant among the quinolone-resistant A. baumannii isolates found in Japan.

Authors+Show Affiliations

Biological Research Laboratories, Daiichi Sankyo, Co., Ltd., Tokyo, Japan; Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, Yamashina, Kyoto, Japan. Electronic address: higuchi.saito.ir@daiichisankyo.co.jp.Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, Yamashina, Kyoto, Japan.Biological Research Laboratories, Daiichi Sankyo, Co., Ltd., Tokyo, Japan.Biological Research Laboratories, Daiichi Sankyo, Co., Ltd., Tokyo, Japan.Department of Microbiology and Infection Control Science, Kyoto Pharmaceutical University, Yamashina, Kyoto, Japan.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

24709045

Citation

Higuchi, Saito, et al. "Characteristics of Antibiotic Resistance and Sequence Type of Acinetobacter Baumannii Clinical Isolates in Japan and the Antibacterial Activity of DS-8587." Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, vol. 20, no. 4, 2014, pp. 256-61.
Higuchi S, Shikata M, Chiba M, et al. Characteristics of antibiotic resistance and sequence type of Acinetobacter baumannii clinical isolates in Japan and the antibacterial activity of DS-8587. J Infect Chemother. 2014;20(4):256-61.
Higuchi, S., Shikata, M., Chiba, M., Hoshino, K., & Gotoh, N. (2014). Characteristics of antibiotic resistance and sequence type of Acinetobacter baumannii clinical isolates in Japan and the antibacterial activity of DS-8587. Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy, 20(4), 256-61. https://doi.org/10.1016/j.jiac.2013.12.001
Higuchi S, et al. Characteristics of Antibiotic Resistance and Sequence Type of Acinetobacter Baumannii Clinical Isolates in Japan and the Antibacterial Activity of DS-8587. J Infect Chemother. 2014;20(4):256-61. PubMed PMID: 24709045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characteristics of antibiotic resistance and sequence type of Acinetobacter baumannii clinical isolates in Japan and the antibacterial activity of DS-8587. AU - Higuchi,Saito, AU - Shikata,Mototsugu, AU - Chiba,Megumi, AU - Hoshino,Kazuki, AU - Gotoh,Naomasa, Y1 - 2014/01/24/ PY - 2013/10/24/received PY - 2013/11/28/revised PY - 2013/12/16/accepted PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2015/1/30/medline KW - Acinetobacter baumannii KW - Antibiotic resistance KW - Efflux pump KW - Quinolone resistance-determining regions KW - Quinolones SP - 256 EP - 61 JF - Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy JO - J Infect Chemother VL - 20 IS - 4 N2 - DS-8587 is a novel broad-spectrum fluoroquinolone with extended antimicrobial activity against both Gram-positive and Gram-negative pathogens. In this study, we evaluated the antibacterial activity and mechanism of DS-8587 in 31 quinolone-resistant Acinetobacter baumannii clinical isolates. Efflux pump and qnr genes, mutations in quinolone resistance-determining regions of target enzymes, and sequence types determined by multilocus sequence typing were analyzed. Forty-two quinolone-susceptible clinical isolates were analyzed for comparison. For susceptibility testing, DS-8587 exhibited more effective antibacterial activity when compared with ciprofloxacin and levofloxacin. When combined with the efflux pump inhibitor 1-(1-napthylmethyl)-piperazine, the MIC of DS-8587 was less affected when compared with the MIC exhibited by combined ciprofloxacin and 1-(1-napthylmethyl)-piperazine. The efflux pump genes adeA/adeB/adeC and regulatory elements adeR/adeS were detected in 23 of 31 quinolone-resistant isolates. The qnrA/qnrB/qnrS genes were not detected in any A. baumannii isolates analyzed. Mutations in quinolone resistance-determining regions were observed in all 31 quinolone-resistant isolates. Multilocus sequence typing analyses revealed that 22 of 31 quinolone-resistant isolates belonged to ST-2, corresponding to international clonal lineage II. In conclusion, DS-8587 exhibits potent antibacterial activity against quinolone-resistant A. baumannii isolates that harbor mutations in quinolone resistance-determining regions. In the presence of the efflux pump inhibitor 1-(1-napthylmethyl)-piperazine, no significant changes were observed in the MIC for DS-8587. DS-8587 should be considered as a treatment option for A. baumannii including ST-2 strains that are predominant among the quinolone-resistant A. baumannii isolates found in Japan. SN - 1437-7780 UR - https://www.unboundmedicine.com/medline/citation/24709045/Characteristics_of_antibiotic_resistance_and_sequence_type_of_Acinetobacter_baumannii_clinical_isolates_in_Japan_and_the_antibacterial_activity_of_DS_8587_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1341-321X(13)00056-1 DB - PRIME DP - Unbound Medicine ER -