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Intermedin in the paraventricular nucleus attenuates cardiac sympathetic afferent reflex in chronic heart failure rats.
PLoS One. 2014; 9(4):e94234.Plos

Abstract

BACKGROUND AND AIM

Intermedin (IMD) is a member of calcitonin/calcitonin gene-related peptide (CGRP) family together with adrenomedullin (AM) and amylin. It has a wide distribution in the central nervous system (CNS) especially in hypothalamic paraventricular nucleus (PVN). Cardiac sympathetic afferent reflex (CSAR) is enhanced in chronic heart failure (CHF) rats. The aim of this study is to determine the effect of IMD in the PVN on CSAR and its related mechanisms in CHF rats.

METHODOLOGY/PRINCIPAL FINDINGS

Rats were subjected to left descending coronary artery ligation to induce CHF or sham-operation (Sham). Renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) were recorded. CSAR was evaluated by the RSNA and MAP responses to epicardial application of capsaicin. Acute experiments were carried out 8 weeks after coronary ligation or sham surgery under anesthesia. IMD and angiotensin II (Ang II) levels in the PVN were up-regulated in CHF rats. Bilateral PVN microinjection of IMD caused greater decreases in CSAR and the baseline RSNA and MAP in CHF rats than those in Sham rats. The decrease of CSAR caused by IMD was prevented by pretreatment with AM receptor antagonist AM22-52, but not CGRP receptor antagonist CGRP8-37. Ang II in the PVN significantly enhanced CSAR and superoxide anions level, which was inhibited by PVN pretreatment with IMD or tempol (a superoxide anions scavenger) in Sham and CHF rats.

CONCLUSION

IMD in the PVN inhibits CSAR via AM receptor, and attenuates the effects of Ang II on CSAR and superoxide anions level in CHF rats. PVN superoxide anions involve in the effect of IMD on attenuating Ang II-induced CSAR response.

Authors+Show Affiliations

Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Physiology, Nanjing Medical University, Nanjing, China; Department of Physiology, Anhui University of Chinese Medicine, Hefei, China.Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Physiology, Nanjing Medical University, Nanjing, China.Department of Physiology, Anhui University of Chinese Medicine, Hefei, China.Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Physiology, Nanjing Medical University, Nanjing, China.Laboratory Center for Basic Medical Sciences, Department of Medical Physiology and Biochemistry, Nanjing Medical University, Nanjing, China.Department of Haematology, the 2nd Affiliated Hospital of Harbin Medical University, Harbin, China.Key Laboratory of Cardiovascular Disease and Molecular Intervention, Department of Physiology, Nanjing Medical University, Nanjing, China.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24709972

Citation

Gan, Xian-Bing, et al. "Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats." PloS One, vol. 9, no. 4, 2014, pp. e94234.
Gan XB, Sun HJ, Chen D, et al. Intermedin in the paraventricular nucleus attenuates cardiac sympathetic afferent reflex in chronic heart failure rats. PLoS ONE. 2014;9(4):e94234.
Gan, X. B., Sun, H. J., Chen, D., Zhang, L. L., Zhou, H., Chen, L. Y., & Zhou, Y. B. (2014). Intermedin in the paraventricular nucleus attenuates cardiac sympathetic afferent reflex in chronic heart failure rats. PloS One, 9(4), e94234. https://doi.org/10.1371/journal.pone.0094234
Gan XB, et al. Intermedin in the Paraventricular Nucleus Attenuates Cardiac Sympathetic Afferent Reflex in Chronic Heart Failure Rats. PLoS ONE. 2014;9(4):e94234. PubMed PMID: 24709972.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intermedin in the paraventricular nucleus attenuates cardiac sympathetic afferent reflex in chronic heart failure rats. AU - Gan,Xian-Bing, AU - Sun,Hai-Jian, AU - Chen,Dan, AU - Zhang,Ling-Li, AU - Zhou,Hong, AU - Chen,Li-Yan, AU - Zhou,Ye-Bo, Y1 - 2014/04/07/ PY - 2013/10/23/received PY - 2014/03/13/accepted PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2014/12/15/medline SP - e94234 EP - e94234 JF - PloS one JO - PLoS ONE VL - 9 IS - 4 N2 - BACKGROUND AND AIM: Intermedin (IMD) is a member of calcitonin/calcitonin gene-related peptide (CGRP) family together with adrenomedullin (AM) and amylin. It has a wide distribution in the central nervous system (CNS) especially in hypothalamic paraventricular nucleus (PVN). Cardiac sympathetic afferent reflex (CSAR) is enhanced in chronic heart failure (CHF) rats. The aim of this study is to determine the effect of IMD in the PVN on CSAR and its related mechanisms in CHF rats. METHODOLOGY/PRINCIPAL FINDINGS: Rats were subjected to left descending coronary artery ligation to induce CHF or sham-operation (Sham). Renal sympathetic nerve activity (RSNA), mean arterial pressure (MAP) and heart rate (HR) were recorded. CSAR was evaluated by the RSNA and MAP responses to epicardial application of capsaicin. Acute experiments were carried out 8 weeks after coronary ligation or sham surgery under anesthesia. IMD and angiotensin II (Ang II) levels in the PVN were up-regulated in CHF rats. Bilateral PVN microinjection of IMD caused greater decreases in CSAR and the baseline RSNA and MAP in CHF rats than those in Sham rats. The decrease of CSAR caused by IMD was prevented by pretreatment with AM receptor antagonist AM22-52, but not CGRP receptor antagonist CGRP8-37. Ang II in the PVN significantly enhanced CSAR and superoxide anions level, which was inhibited by PVN pretreatment with IMD or tempol (a superoxide anions scavenger) in Sham and CHF rats. CONCLUSION: IMD in the PVN inhibits CSAR via AM receptor, and attenuates the effects of Ang II on CSAR and superoxide anions level in CHF rats. PVN superoxide anions involve in the effect of IMD on attenuating Ang II-induced CSAR response. SN - 1932-6203 UR - https://www.unboundmedicine.com/medline/citation/24709972/Intermedin_in_the_paraventricular_nucleus_attenuates_cardiac_sympathetic_afferent_reflex_in_chronic_heart_failure_rats_ L2 - http://dx.plos.org/10.1371/journal.pone.0094234 DB - PRIME DP - Unbound Medicine ER -