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Detection of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in wild small mammals in Spain.
J Antimicrob Chemother. 2014 Aug; 69(8):2061-4.JA

Abstract

OBJECTIVES

To determine the rate of Staphylococcus aureus faecal carriage in 101 wild small mammals in Spain and to characterize the isolates obtained.

METHODS

Faecal samples were seeded on mannitol salt agar and ORSAB plates. The presence of the resistance genes mecA, mecC and blaZ and the new blaZ allotype associated with staphylococcal cassette chromosome mec (SCCmec) XI (blaZ-SCCmecXI) was studied by PCR. S. aureus isolates were characterized by spa typing, agr typing and multilocus sequence typing. The presence of immune evasion cluster (IEC) genes and virulence genes was analysed by PCR.

RESULTS

S. aureus was detected in 13/101 studied faecal samples and one isolate per positive sample was further studied. Two S. aureus isolates were methicillin-resistant S. aureus (MRSA) (recovered from wood mice, Apodemus sylvaticus) and 11 were methicillin-susceptible S. aureus (MSSA). Both MRSA isolates harboured the mecC gene and the novel blaZ-SCCmecXI, were typed as spa-t1535/agrIII/ST1945(CC130)/SCCmecXI (where ST stands for sequence type and CC stands for clonal complex), carried the exfoliative toxin etd2 gene and were IEC type E. Eight different spa types were identified among the 11 MSSA isolates (five new) and six different sequence types were identified (two new). All MSSA strains were susceptible to the antibiotics tested except one blaZ-positive penicillin-resistant isolate (spa-t120/agrII/ST15). MSSA isolates were ascribed to the CCs (number of strains) CC5 (1), CC1956 (4) and singleton (6). Nine of 11 MSSA isolates carried the cna virulence gene. Only one MSSA isolate carried IEC genes (type C).

CONCLUSIONS

This is the first report of MRSA carrying mecC in faecal samples of wild small mammals in Spain. These resistant isolates carried genes of the IEC system, unusual in S. aureus from animals. Wild small mammals could be a reservoir of the mecC gene with important implications for public health.

Authors+Show Affiliations

Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.SaBio group, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.Ecology group, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Ecology group, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain.SaBio group, IREC (CSIC-UCLM-JCCM), Ciudad Real, Spain.Area of Biochemistry and Molecular Biology, University of La Rioja, Logroño, Spain carmen.torres@unirioja.es.

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

24710026

Citation

Gómez, Paula, et al. "Detection of Methicillin-resistant Staphylococcus Aureus (MRSA) Carrying the mecC Gene in Wild Small Mammals in Spain." The Journal of Antimicrobial Chemotherapy, vol. 69, no. 8, 2014, pp. 2061-4.
Gómez P, González-Barrio D, Benito D, et al. Detection of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in wild small mammals in Spain. J Antimicrob Chemother. 2014;69(8):2061-4.
Gómez, P., González-Barrio, D., Benito, D., García, J. T., Viñuela, J., Zarazaga, M., Ruiz-Fons, F., & Torres, C. (2014). Detection of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in wild small mammals in Spain. The Journal of Antimicrobial Chemotherapy, 69(8), 2061-4. https://doi.org/10.1093/jac/dku100
Gómez P, et al. Detection of Methicillin-resistant Staphylococcus Aureus (MRSA) Carrying the mecC Gene in Wild Small Mammals in Spain. J Antimicrob Chemother. 2014;69(8):2061-4. PubMed PMID: 24710026.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene in wild small mammals in Spain. AU - Gómez,Paula, AU - González-Barrio,David, AU - Benito,Daniel, AU - García,Jesús T, AU - Viñuela,Javier, AU - Zarazaga,Myriam, AU - Ruiz-Fons,Francisco, AU - Torres,Carmen, Y1 - 2014/04/07/ PY - 2014/4/9/entrez PY - 2014/4/9/pubmed PY - 2015/10/1/medline KW - CC30 KW - ST1945 KW - clonal complex 30 KW - sequence type 1945 KW - wood mouse SP - 2061 EP - 4 JF - The Journal of antimicrobial chemotherapy JO - J Antimicrob Chemother VL - 69 IS - 8 N2 - OBJECTIVES: To determine the rate of Staphylococcus aureus faecal carriage in 101 wild small mammals in Spain and to characterize the isolates obtained. METHODS: Faecal samples were seeded on mannitol salt agar and ORSAB plates. The presence of the resistance genes mecA, mecC and blaZ and the new blaZ allotype associated with staphylococcal cassette chromosome mec (SCCmec) XI (blaZ-SCCmecXI) was studied by PCR. S. aureus isolates were characterized by spa typing, agr typing and multilocus sequence typing. The presence of immune evasion cluster (IEC) genes and virulence genes was analysed by PCR. RESULTS: S. aureus was detected in 13/101 studied faecal samples and one isolate per positive sample was further studied. Two S. aureus isolates were methicillin-resistant S. aureus (MRSA) (recovered from wood mice, Apodemus sylvaticus) and 11 were methicillin-susceptible S. aureus (MSSA). Both MRSA isolates harboured the mecC gene and the novel blaZ-SCCmecXI, were typed as spa-t1535/agrIII/ST1945(CC130)/SCCmecXI (where ST stands for sequence type and CC stands for clonal complex), carried the exfoliative toxin etd2 gene and were IEC type E. Eight different spa types were identified among the 11 MSSA isolates (five new) and six different sequence types were identified (two new). All MSSA strains were susceptible to the antibiotics tested except one blaZ-positive penicillin-resistant isolate (spa-t120/agrII/ST15). MSSA isolates were ascribed to the CCs (number of strains) CC5 (1), CC1956 (4) and singleton (6). Nine of 11 MSSA isolates carried the cna virulence gene. Only one MSSA isolate carried IEC genes (type C). CONCLUSIONS: This is the first report of MRSA carrying mecC in faecal samples of wild small mammals in Spain. These resistant isolates carried genes of the IEC system, unusual in S. aureus from animals. Wild small mammals could be a reservoir of the mecC gene with important implications for public health. SN - 1460-2091 UR - https://www.unboundmedicine.com/medline/citation/24710026/Detection_of_methicillin_resistant_Staphylococcus_aureus__MRSA__carrying_the_mecC_gene_in_wild_small_mammals_in_Spain_ L2 - https://academic.oup.com/jac/article-lookup/doi/10.1093/jac/dku100 DB - PRIME DP - Unbound Medicine ER -